1887

Abstract

A polyphasic study was performed to determine the taxonomic position of two strains, 665N and 868E, isolated from bivalve molluscs, that could not be identified at the species level in a previous numerical taxonomy study. The DNA G+C content of these isolates was 62.3 and 62.6 mol%, respectively. Sequence analysis of the 16S rRNA gene showed that the two new strains were closely related to members of the genus . Fluorescence amplified fragment length polymorphism fingerprinting revealed that strains 665N and 868E clustered together with a similarity of 78 % but did not cluster with any of the genomospecies. DNA–DNA hybridization experiments revealed a high level of relatedness between the two new isolates (76 %) but low levels of relatedness between these and phylogenetically most closely related genomospecies (30–44 %). Useful tests for the phenotypic differentiation of strains 665N and 868E from other mesophilic species included those for gas from glucose, lysine decarboxylase, Voges–Proskauer reaction, acid from -arabinose, hydrolysis of aesculin and utilization of -lactate. On the basis of genotypic and phenotypic evidence, strains 665N and 868E are considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 868E (=CECT 7113=LMG 23376).

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2007-03-01
2020-01-22
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vol. , part 3, pp. 582 - 587

DNA–DNA hybridization between strain 868E and strain 665N and type or reference strains of the phylogenetically more related hybridization groups (HG). [PDF](14 KB)



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