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Abstract

Bacterial strain LW4 was isolated from activated sludge of a wastewater-treatment bioreactor. Cells of strain LW4 were Gram-positive cocci, with a diameter of 0.7–1.0 μm. Colonies produced on LB agar plates were yellow, smooth, circular and 0.5–1.5 mm in diameter. Strain LW4 was aerobic and grew over the temperature range 26–34 °C and pH range 5–9, with optimal growth at 30.5–31.5 °C and pH 6.0–6.2. The cell-wall peptidoglycan of strain LW4 contained amino acid residues of lysine, glutamic acid, alanine, glycine and aspartic acid. The most abundant cellular fatty acids of strain LW4 were anteiso-C (32.15 %) and iso-C (31.65 %). Major respiratory quinones were MK-8(H) (57.3 %) and MK-7(H) (32.9 %). The DNA G+C content was 71.4 mol% ( ). 16S rRNA gene sequence analysis indicated that strain LW4 was phylogenetically related to members of the genus , with similarities ranging from 96.5 to 97.3 %. Levels of DNA–DNA relatedness of strain LW4 to DSM 20030, DSM 20315 and AS 1.2372 were 55, 48 and 36 %, respectively. Based on these results, it is concluded that strain LW4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain LW4 (=CGMCC 1.5361=JCM 14000).

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2007-01-01
2019-10-17
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Scanning electron micrograph of cells of sp. nov. LW4 grown on LB agar at 30 °C for 3 days. Bar, 2 µm.

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Cellular fatty acid composition (%) of sp. nov. LW4 . [PDF](43 KB)

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