1887

Abstract

A Gram-positive, rod-shaped, non-motile bacterium, designated strain wged11, was isolated from the root of ginseng, and its taxonomic position was established using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that this organism formed a robust clade with recognized species of the genus . Strain wged11 was characterized by a high content of -cyclohexylundecanoic and anteiso- and iso-branched saturated fatty acids, MK-11 as the major menaquinone and -2,4-diaminobutyric acid in its cell-wall peptidoglycan. The DNA G+C content of strain wged11 was 66.4 mol%. Levels of similarity between the 16S rRNA gene sequence of strain wged11 and those of the type strains of other members of the genus ranged from 94.7 to 97.6 %. The mean level of DNA–DNA relatedness between strain wged11 and DSM 15202, its nearest phylogenetic neighbour, was 35.3 %. Based on these findings, strain wged11 (=CGMCC 4.3491=JCM 13908) is proposed as the type strain of a novel species of the genus , sp. nov.

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2007-02-01
2024-03-29
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References

  1. Bousfield I. J., Keddie R. M., Dando T. R., Shaw S. 1985; Simple rapid methods of cell wall analysis as an aid in the identification of aerobic coryneform bacteria. In Chemical Methods in Bacterial Systematics pp  221–236 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  2. Collins M. D. 1985; Isoprenoid quinine analysis in classification and identification. In Chemical Methods in Bacterial Systematics pp  267–287 Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  3. Davis M. J., Gillaspie A. G., Vidaver A. K., Harris R. W. 1984; Clavibacter : a new genus containing some phytopathogenic coryneform bacteria, including Clavibacter xyli subsp.xyli sp. nov., subsp. nov. and Clavibacterxyli subsp. cynodontis subsp. nov., pathogens that cause ratoon stunting disease of sugarcane and bermudagrass stunting disease. Int J Syst Bacteriol 34:107–117 [CrossRef]
    [Google Scholar]
  4. Dong X., Xin Y., Jian W., Liu X., Ling D. 2000; Bifidobacterium thermacidophilum sp. nov., isolated from an anerobic digester. Int J Syst Evol Microbiol 50:119–125 [CrossRef]
    [Google Scholar]
  5. Eck R. V., Dayhoff M. O. 1966 Atlas of Protein Sequence and Structure Silver Springs, MD: National Biomedical Research Foundation;
    [Google Scholar]
  6. Evtushenko L. I., Dorofeeva L. V., Subbotin S. A., Cole J. R., Tiedje J. M. 2000; Leifsonia poae gen. nov., sp. nov., isolated from nematode galls on Poa annua , and reclassification of ‘ Corynebacterium aquaticum ’ Leifson 1962 as Leifsonia aquatica ( ex Leifson 1962) gen. nov., nom. rev., comb. nov. and Clavibacter xyli Davis et al . 1984 with two subspecies as Leifsonia xyli (Davis et al . 1984) gen. nov., comb. nov. Int J Syst Evol Microbiol 50:371–380 [CrossRef]
    [Google Scholar]
  7. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  8. Felsenstein J., Churchill G. A. 1996; A Hidden Markov Model approach to variation among sites in rate of evolution. Mol Biol Evol 13:93–104 [CrossRef]
    [Google Scholar]
  9. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [CrossRef]
    [Google Scholar]
  10. Gledhill W. E., Casida L. E. Jr 1969; Predominant catalase-negative soil bacteria. III. Agromyces , gen. n.,microorganisms intermediary to Actinomyces and Nocardia . Appl Environ Microbiol 18:340–349
    [Google Scholar]
  11. Hugh R., Leifson E. 1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol 66:24–26
    [Google Scholar]
  12. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [CrossRef]
    [Google Scholar]
  13. Kawaguchi A., Uemura N., Okuda S. 1986; Characterization of the fatty acid synthetase system of Curtobacterium pusillum . J Biochem (Tokyo 99:1735–1742
    [Google Scholar]
  14. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp  115–175 Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  15. Leifson E. 1962; The bacterial flora of distilled and stored water III. New species of the genera Corynebacterium , Flavobacterium , Spirillum and Pseudomonas . Int Bull Bacteriol Nomencl Taxon 12:161–170
    [Google Scholar]
  16. Ludwig W., Strunk O., Klugbauer S., Klugbauer N., Weizenegger M., Neumaier J., Bachleitner M., Schleifer K. H. 1998; Bacterial phylogeny based on comparative sequence analysis. Electrophoresis 19:554–568 [CrossRef]
    [Google Scholar]
  17. Maltsev I. I., Kalinovskii A. I., Zgurskaya H. I., Evtushenko L. I. 1992; Tyvelose in Agromyces cell walls. Syst Appl Microbiol 15:187–189 [CrossRef]
    [Google Scholar]
  18. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  19. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  20. Reddy G. S. N., Prakash J. S. S., Srinivas R., Matsumoto G. I., Shivaji S. 2003; Leifsonia rubra sp. nov. and Leifsonia aurea sp. nov., psychrophiles from a pond in Antarctica. Int J Syst Evol Microbiol 53:977–984 [CrossRef]
    [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  22. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids , MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  23. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  24. Streshinskaya G. M., Naumova I. B., Panina L. I. 1979; Cell wall composition of Streptomyces chrysomallus producing the antibiotic aurantin. Mikrobiologiia 48:814–819
    [Google Scholar]
  25. Suzuki K. I., Suzuki M., Sasaki J., Park Y. H., Komagata K. 1999; Leifsonia gen. nov., a genus for 2,4-diaminobutyric acid-containing actinomycetes to accommodate “ Corynebacterium aquaticum ” Leifson 1962 and Clavibacter xyli susp. cynodontis Davis et al . 1984. J Gen Appl Microbiol 45:253–262 [CrossRef]
    [Google Scholar]
  26. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  27. Von Bulow J. F. W., Döbereiner J. 1975; Potential for nitrogen fixation in maize genotypes in Brazil. Proc Natl Acad Sci U S A 72:2389–2393 [CrossRef]
    [Google Scholar]
  28. Wu C., Lu X., Qin M., Wang Y., Ruan J. 1989; Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing) ] 16:176–178
    [Google Scholar]
  29. Zgurskaya H. I., Evtushenko L. I., Akimov V. N., Kalakoutskii L. V. 1993; Rathayibacter gen. nov., including the species Rathayibacter rathayi comb. nov., Rathayibacter tritici comb. nov., Rathayibacter iranicus comb. nov., and six strains from annual grasses. Int J Syst Bacteriol 43:143–149 [CrossRef]
    [Google Scholar]
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