1887

Abstract

Strain B538 is a Gram-positive, motile, rod-shaped bacterium, which was isolated from Xinjiang province in China. This organism grew optimally at 30–35 °C and pH 8.0–8.5. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain B538 belonged to the genus and chemotaxonomic data (DNA G+C content, 47.0 mol%; major isoprenoid quinone, MK-7; cell wall type, A1 -diaminopimelic acid; major fatty acids, anteiso-C and C) supported affiliation of the isolate with the genus . Comparative 16S rRNA gene sequence analyses showed that the isolate was most closely related to DS-1, with 16S rRNA gene sequence similarity of 98.1 %; sequence similarities to other members of the genus used in the phylogenetic tree were less than 96.5 %. The DNA–DNA relatedness between strain B538 and DS-1 was about 8.0 %. On the basis of physiological and molecular properties, strain B538 (=KCTC 3952=DSM 16970) is proposed as the type strain of a novel species within the genus , for which the name sp. nov. is proposed.

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2006-11-01
2020-01-27
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References

  1. Ash, C., Priest, F. G. & Collins, M. D. ( 1993; ). Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Antonie van Leeuwenhoek 64, 253–260.
    [Google Scholar]
  2. Cole, J. R., Chai, B., Marsh, T. L. & 8 other authors ( 2003; ). The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res 31, 442–443.[CrossRef]
    [Google Scholar]
  3. Daane, L. L., Harjono, I., Barns, S. M., Launen, L. A., Palleroni, N. J. & Häggblom, M. M. ( 2002; ). PAH-degradation by Paenibacillus spp. and description of Paenibacillus naphthalenovorans sp. nov., a naphthalene-degrading bacterium from the rhizosphere of salt marsh plants. Int J Syst Evol Microbiol 52, 131–139.
    [Google Scholar]
  4. Dasman, Kajiyama, S., Kawasaki, H., Yagi, M., Seki, T., Fukusaki, E. & Kobayashi, A. ( 2002; ). Paenibacillus glycanilyticus sp. nov., a novel species that degrades heteropolysaccharide produced by the cyanobacterium Nostoc commune. Int J Syst Evol Microbiol 52, 1669–1674.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 2002; ). phylip (phylogenetic inference package), version 3.6a. Distributed by the author. University of Washington, Seattle, USA.
  6. Gomori, G. ( 1955; ). Preparation of buffers for use in enzyme studies. Methods Enzymol 1, 138–146.
    [Google Scholar]
  7. Jeon, C. O., Lim, J.-M., Lee, J. C., Lee, G. S., Lee, J. M., Xu, L. H., Jiang, C. L. & Kim, C.-J. ( 2005a; ). Lentibacillus salarius sp. nov., isolated from saline sediment in China, and emended description of the genus Lentibacillus. Int J Syst Evol Microbiol 55, 1339–1343.[CrossRef]
    [Google Scholar]
  8. Jeon, C. O., Lim, J. M., Lee, J. M., Xu, L. H., Jiang, C. L. & Kim, C.-J. ( 2005b; ). Reclassification of Bacillus haloalkaliphilus Fritze 1996 as Alkalibacillus haloalkaliphilus gen. nov., comb. nov. and the description of Alkalibacillus salilacus sp. nov., a novel halophilic bacterium isolated from a salt lake in China. Int J Syst Evol Microbiol 55, 1891–1896.[CrossRef]
    [Google Scholar]
  9. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  10. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–206.
    [Google Scholar]
  11. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: John Wiley.
  12. Lanyi, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  13. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  14. Lim, J.-M., Jeon, C. O., Park, D. J., Kim, H. R., Yoon, B. J. & Kim, C.-J. ( 2005; ). Pontibacillus marinus sp. nov., a moderately halophilic bacterium from a solar saltern, and emended description of the genus Pontibacillus. Int J Syst Evol Microbiol 55, 1027–1031.[CrossRef]
    [Google Scholar]
  15. Montes, M. J., Mercade, E., Bozal, N. & Guinea, J. ( 2004; ). Paenibacillus antarcticus sp. nov., a novel psychrotolerant organism from the Antarctic environment. Int J Syst Evol Microbiol 54, 1521–1526.[CrossRef]
    [Google Scholar]
  16. Rivas, R., Mateos, P. F., Martínez-Molina, E. & Velázquez, E. ( 2005a; ). Paenibacillus xylanilyticus sp. nov., an airborne xylanolytic bacterium. Int J Syst Evol Microbiol 55, 405–408.[CrossRef]
    [Google Scholar]
  17. Rivas, R., Mateos, P. F., Martínez-Molina, E. & Velázquez, E. ( 2005b; ). Paenibacillus phyllosphaerae sp. nov., a xylanolytic bacterium isolated from the phyllosphere of Phoenix dactylifera. Int J Syst Evol Microbiol 55, 743–746.[CrossRef]
    [Google Scholar]
  18. Rivas, R., Gutiérrez, C., Abril, A., Mateos, P. F., Martínez-Molina, E., Ventosa, A. & Velázquez, E. ( 2005c; ). Paenibacillus rhizosphaerae sp. nov., isolated from the rhizosphere of Cicer arietinum. Int J Syst Evol Microbiol 55, 1305–1309.[CrossRef]
    [Google Scholar]
  19. Saha, P., Mondal, A. K., Mayilraj, S., Krishnamurthi, S., Bhattacharya, A. & Chakrabarti, T. ( 2005; ). Paenibacillus assamensis sp. nov., a novel bacterium isolated from a warm spring in Assam, India. Int J Syst Evol Microbiol 55, 2577–2581.[CrossRef]
    [Google Scholar]
  20. Sambrook, J. & Russell, D. W. ( 2001; ). Molecular Cloning: a Laboratory Manual, 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  21. Sánchez, M. M., Fritze, D., Blanco, A., Spröer, C., Tindall, B. J., Schumann, P., Kroppenstedt, R. M., Diaz, P. & Pastor, F. I. J. ( 2005; ). Paenibacillus barcinonensis sp. nov., a xylanase-producing bacterium isolated from a rice field in the Ebro River delta. Int J Syst Evol Microbiol 55, 935–939.[CrossRef]
    [Google Scholar]
  22. Shida, O., Takagi, H., Kadowaki, K., Nakamura, L. K. & Komagata, K. ( 1997; ). Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus. Int J Syst Bacteriol 47, 289–298.[CrossRef]
    [Google Scholar]
  23. Šmerda, J., Sedláček, I., Páčová, Z., Durnová, E., Smíšková, A. & Havel, L. ( 2005; ). Paenibacillus mendelii sp. nov., from surface-sterilized seeds of Pisum sativum L. Int J Syst Evol Microbiol 55, 2351–2354.[CrossRef]
    [Google Scholar]
  24. Smibert, R. M. & Krieg, N. R. ( 1981; ). General characterization. In Manual of Methods for General Bacteriology, pp. 409–443. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Washington, DC: American Society for Microbiology.
  25. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  26. Stackebrandt, E., Frederiksen, W., Garrity, G. M. & 10 other authors ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52, 1043–1047.[CrossRef]
    [Google Scholar]
  27. Takeda, M., Suzuki, I. & Koizumi, J. ( 2005; ). Paenibacillus hodogayensis sp. nov., capable of degrading the polysaccharide produced by Sphaerotilus natans. Int J Syst Evol Microbiol 55, 737–741.[CrossRef]
    [Google Scholar]
  28. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  29. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  30. Uetanabaro, A. P., Wahrenburg, C., Hunger, W., Pukall, R., Spröer, C., Stackebrandt, E., De Canhos, V. P., Claus, D. & Fritze, D. ( 2003; ). Paenibacillus agarexedens sp. nov., nom. rev., and Paenibacillus agaridevorans sp. nov. Int J Syst Evol Microbiol 53, 1051–1057.[CrossRef]
    [Google Scholar]
  31. van der Maarel, M. J. E. C., Veen, A. & Wijbenga, D. J. ( 2000; ). Paenibacillus granivorans sp. nov., a new Paenibacillus species which degrades native potato starch granules. Syst Appl Microbiol 23, 344–348.[CrossRef]
    [Google Scholar]
  32. Yoon, J.-H., Kang, S.-J., Yeo, S.-H. & Oh, T.-K. ( 2005; ). Paenibacillus alkaliterrae sp. nov., isolated from an alkaline soil in Korea. Int J Syst Evol Microbiol 55, 2339–2344.[CrossRef]
    [Google Scholar]
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Transmission electron micrograph showing the general morphology of negatively stained cells of strain B538 after growth for 2 days at 32 °C in TSB broth. Bar, 1 µm.

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Fatty acid compositions of strain BH538 and related type strains. [PDF](49 KB)

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