1887

Abstract

The taxonomic position of six strains isolated from swine waste-corn fermentations was reinvestigated. All strains were included in a multilocus sequence analysis (MLSA) study for species identification of using the genes encoding the phenylalanyl-tRNA synthase alpha subunit () and RNA polymerase alpha subunit (). Partial and gene sequences showed that strains LMG 11400 and NRRL B-4435 represent a separate lineage that is distantly related to the type strain of , LMG 6900, and to three other strains of the species. The MLSA data showed that the two strains LMG 11400 and NRRL B-4435 constituted a distinct cluster, sharing 100 % and gene sequence similarity. The other reference strains clustered together with the type strain of , LMG 6900, and were clearly differentiated from strains LMG 11400 and NRRL B-4435 (80 and 89 % and gene sequence similarity, respectively). The 16S rRNA gene sequences of the latter two strains are 100 % identical, with the nearest phylogenetic neighbour LMG 6900 showing only 97.2 % 16S rRNA gene sequence similarity. Further polyphasic taxonomic study based on whole-cell protein fingerprinting, DNA–DNA hybridization and biochemical features demonstrated that the two strains represent a single, novel species, for which the name sp. nov. is proposed. The type strain is LMG 11400 (=NRRL B-4436=DSM 20534).

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2006-11-01
2020-01-28
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vol. , part 11, pp. 2523 - 2527

Distance-matrix tree showing phylogenetic relationships of strains LMG 11400 and NRRL B-4435 ( sp. nov.), strains and other reference species based on 16S rRNA gene sequence comparisons. [PDF](55 KB)



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