@article{mbs:/content/journal/ijsem/10.1099/ijs.0.64460-0, author = "Ryu, Seung Hyun and Nguyen, Thi Thu Hien and Park, Woojun and Kim, Chang-Jin and Jeon, Che Ok", title = "Runella limosa sp. nov., isolated from activated sludge", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2006", volume = "56", number = "12", pages = "2757-2760", doi = "https://doi.org/10.1099/ijs.0.64460-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64460-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A Gram-negative bacterium, designated strain EMB111T, was isolated from activated sludge performing enhanced biological phosphorus removal in a sequencing batch reactor. Cells were long and rod-shaped. The isolate was strictly aerobic and non-motile. The strain grew optimally at 25–30 °C and pH 7.5–8.0. The predominant fatty acids of strain EMB111T were iso-C15 : 0, C16 : 1 ω5c, iso-C17 : 0 3-OH, iso-C15 : 0 3-OH, C16 : 0 3-OH, C16 : 0 and summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH). The strain contained a large amount of phosphatidylglycerol and small amounts of two unknown phospholipids (PL1, PL2) as the polar lipids. The major isoprenoid quinone was menaquinone-7. The G+C content of the genomic DNA was 42.7 mol%. Phylogenetic analysis showed that strain EMB111T formed a phyletic cluster with members of the genus Runella within the family Flexibacteraceae and was most closely related to Runella slithyformis ATCC 29530T with a 16S rRNA gene sequence similarity of 94.8 %. On the basis of chemotaxonomic data and molecular properties, strain EMB111T represents a novel species within the genus Runella, for which the name Runella limosa sp. nov. is proposed. The type strain is EMB111T (=KCTC 12615T=DSM 17973T).", }