1887

Abstract

A Gram-negative bacterium, designated strain EMB111, was isolated from activated sludge performing enhanced biological phosphorus removal in a sequencing batch reactor. Cells were long and rod-shaped. The isolate was strictly aerobic and non-motile. The strain grew optimally at 25–30 °C and pH 7.5–8.0. The predominant fatty acids of strain EMB111 were iso-C, C , iso-C 3-OH, iso-C 3-OH, C 3-OH, C and summed feature 3 (C 7 and/or iso-C 2-OH). The strain contained a large amount of phosphatidylglycerol and small amounts of two unknown phospholipids (PL1, PL2) as the polar lipids. The major isoprenoid quinone was menaquinone-7. The G+C content of the genomic DNA was 42.7 mol%. Phylogenetic analysis showed that strain EMB111 formed a phyletic cluster with members of the genus within the family and was most closely related to ATCC 29530 with a 16S rRNA gene sequence similarity of 94.8 %. On the basis of chemotaxonomic data and molecular properties, strain EMB111 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is EMB111 (=KCTC 12615=DSM 17973).

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2006-12-01
2019-10-18
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vol. , part 12, pp. 2757 - 2759

Transmission electron micrograph showing the general morphology of negatively stained cells of strain EMB111 after growth for 3 days at 25 °C on R2A broth. Bar, 10 µm.



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