RT Journal Article SR Electronic(1) A1 Zhang, Li A1 Xu, Zhihong A1 Patel, Bharat K. C.YR 2007 T1 Bacillus decisifrondis sp. nov., isolated from soil underlying decaying leaf foliage JF International Journal of Systematic and Evolutionary Microbiology, VO 57 IS 5 SP 974 OP 978 DO https://doi.org/10.1099/ijs.0.64440-0 PB Microbiology Society, SN 1466-5034, AB An aerobic bacterium, designated strain E5HC-32T, was isolated from soil underlying the decaying leaf litter of a slash pine forest located in south east Queensland, Australia. The strictly aerobic, motile rod-shaped cells (0.8–1.6×2.6–4.8 μm) produced subterminal spherical spores which distended the cells. Strain E5HC-32T grew optimally in 1 % trypticase soy broth (TSB) at 30 °C (temperature range for growth, 25–40 °C) and a pH of 8.4 (pH growth range, pH 7.1–9.1). Electron microscopic examination of negatively stained cells revealed the presence of peritrichous flagella and thin sections showed the presence of a typical Gram-positive type cell-wall ultrastructure. The strain was catalase-positive and oxidase-negative and metabolized pyruvic acid methyl ester, d-galactonic acid lactone, α-ketobutyric acid, α-ketovaleric acid, l-proline, l-alanine, urocanic acid, inosine, uridine, thymidine, glycerol, α-cyclodextrin, α-d-lactose, d-psicose, d-raffinose, l-rhamnose, d-sorbitol, turanose, cis-aconitic acid, α-hydroxybutyric acid, l-alaninamide and 2-aminoethanol. The G+C content of DNA was 41±1 mol% as determined by the thermal denaturation method. 16S rRNA gene sequence analysis revealed that strain E5HC-32T was placed equidistantly as a member of the class Bacilli, phylum Firmicutes, with Bacillus sphaericus DSM 28T and Bacillus odysseyi ATCC PTA-4993T (similarity of 93 %). In addition to its significant phylogenetic separation from its nearest relatives, strain E5HC-32T possessed phenotypic traits that also suggested that it represented a novel species, for which the name Bacillus decisifrondis sp. nov. is proposed. The type strain is E5HC-32T (=JCM 13601T=DSM 17725T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.64440-0