1887

Abstract

An aerobic bacterium, designated strain E5HC-32, was isolated from soil underlying the decaying leaf litter of a slash pine forest located in south east Queensland, Australia. The strictly aerobic, motile rod-shaped cells (0.8–1.6×2.6–4.8 μm) produced subterminal spherical spores which distended the cells. Strain E5HC-32 grew optimally in 1 % trypticase soy broth (TSB) at 30 °C (temperature range for growth, 25–40 °C) and a pH of 8.4 (pH growth range, pH 7.1–9.1). Electron microscopic examination of negatively stained cells revealed the presence of peritrichous flagella and thin sections showed the presence of a typical Gram-positive type cell-wall ultrastructure. The strain was catalase-positive and oxidase-negative and metabolized pyruvic acid methyl ester, -galactonic acid lactone, -ketobutyric acid, -ketovaleric acid, -proline, -alanine, urocanic acid, inosine, uridine, thymidine, glycerol, -cyclodextrin, --lactose, -psicose, -raffinose, -rhamnose, -sorbitol, turanose, -aconitic acid, -hydroxybutyric acid, -alaninamide and 2-aminoethanol. The G+C content of DNA was 41±1 mol% as determined by the thermal denaturation method. 16S rRNA gene sequence analysis revealed that strain E5HC-32 was placed equidistantly as a member of the class , phylum , with DSM 28 and ATCC PTA-4993 (similarity of 93 %). In addition to its significant phylogenetic separation from its nearest relatives, strain E5HC-32 possessed phenotypic traits that also suggested that it represented a novel species, for which the name sp. nov. is proposed. The type strain is E5HC-32 (=JCM 13601=DSM 17725).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64440-0
2007-05-01
2024-12-10
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/5/974.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64440-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
    [Google Scholar]
  2. Andrews K. T., Patel B. K. C. 1996; Fervidobacterium gondwanense sp. nov., a new thermophilic anaerobic bacterium isolated from nonvolcanically heated geothermal waters of the Great Artesian Basin of Australia. Int J Syst Bacteriol 46:265–269 [CrossRef]
    [Google Scholar]
  3. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D. 1991; Phylogenetic heterogeneity of the genus Bacillus as revealed by comparative analysis of small-subunit-ribosomal RNA sequences. Lett Appl Microbiol 13:202–206
    [Google Scholar]
  4. Chester F. D. 1898; Report of the mycologist: bacteriological work. Del Agric Exp Stn Annu Rep 10:47–137
    [Google Scholar]
  5. Cole J. R., Chai B., Farris R. J., Wang Q., Kulam S. A., McGarrell D. M., Garrity G. M., Tiedje J. M. 2005; The Ribosomal Database Project (RDP-II): sequences and tools for high-throughput rRNA analysis. Nucleic Acids Res 33(Database issue), D294–D296:
    [Google Scholar]
  6. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  7. Fortina M. G., Pukall R., Schumann P., Mora D., Parini C. P., Manachini L., Stackebrandt E. 2001; Ureibacillus gen. nov., a new genus to accommodate Bacillus thermosphaericus (Andersson et al. 1995), emendation of Ureibacillus thermosphaericus and description of Ureibacillus terrenus sp. nov. Int J Syst Evol Microbiol 51:447–455
    [Google Scholar]
  8. Garrity G. M., Holt J. G. 2001; The road map to the Manual . In Bergey's Manual of Systematic Bacteriology . , 2nd edn. vol 1 The Archaea and the Deeply Branching and Phototrophic Bacteria pp  119–166 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer;
    [Google Scholar]
  9. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  10. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol  3 pp  21–123 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  11. Kanso S., Patel B. K. C. 2003 Microvirga subterranea gen. nov., sp. nov., a moderate thermophile from a deep subsurface Australian thermal aquifer. Int J Syst Evol Microbiol 53401–406 [CrossRef]
  12. La Duc M. T., Satomi M., Venkateswaran K. 2004; Bacillus odysseyi sp nov., a round-spore-forming bacillus isolated from the Mars Odyssey spacecraft. Int J Syst Evol Microbiol 54:195–201 [CrossRef]
    [Google Scholar]
  13. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar Buchner A., Lai T., Steppi S. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  14. Marmur J., Doty P. 1961; Thermal renaturation of deoxyribonucleic acids. J Mol Biol 3:585–594 [CrossRef]
    [Google Scholar]
  15. Nakamura L. K. 2000; Phylogeny of Bacillus sphaericus -like organisms. Int J Syst Evol Microbiol 50:1715–1722
    [Google Scholar]
  16. Nakamura L. K., Shida O., Takagi H., Komagata K. 2002; Bacillus pycnus sp. nov. and Bacillus neidei sp. nov round-spored bacteria from soil. Int J Syst Evol Microbiol 52:501–505
    [Google Scholar]
  17. Neide E. 1904; Botanische Beschreibung einiger sporebildenden Bakterien. Zentralbl Bakteriol Parasitenkd Infektionskr Hyg Abt. II 12337–352
  18. Priest F. G., Goodfellow M., Todd C. 1988; A numerical classification of the genus Bacillus . J Gen Microbiol 134:1847–1882
    [Google Scholar]
  19. Rheims H., Fruhling A., Schumann P., Rohde M., Stackebrandt E. 1999; Bacillus silvestris sp. nov., a new member of the genus Bacillus that contains lysine in its cell wall. Int J Syst Bacteriol 49:795–802 [CrossRef]
    [Google Scholar]
  20. Sait M., Hugenholtz P., Janssen P. H. 2002; Cultivation of globally distributed soil bacteria from phylogenetic lineages previously only detected in cultivation-independent surveys. Environ Microbiol 4:654–666 [CrossRef]
    [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  22. Spanevello M. D., Yamamoto H., Patel B. K. 2002; Thermaerobacter subterraneus sp. nov., a novel aerobic bacterium from the Great Artesian Basin of Australia, and emendation of the genus Thermaerobacter . Int J Syst Evol Microbiol 52:795–800 [CrossRef]
    [Google Scholar]
  23. Tiedje J. M., Asuming-Brempong S., Nüsslein K., March T. L., Flynn S. J. 1999; Opening the black box of soil microbial diversity. Appl Soil Ecol 13:109–122 [CrossRef]
    [Google Scholar]
  24. Van de Peer Y., Jansen J., De Rijk P., De Wachter P. 1997; Database on the structure of small ribosomal subunit RNA. Nucleic Acids Res 25:111–116 [CrossRef]
    [Google Scholar]
  25. Yoon J. H., Lee K. C., Weiss N., Kho Y. H., Kang K. H., Park Y. H. 2001; Sporosarcina aquimarina sp. nov., a bacterium isolated from seawater in Korea, and transfer of Bacillus globisporus (Larkin and Stokes 1967), Bacillus psychrophilus (Nakamura 1984) and Bacillus pasteurii (Chester 1898) to the genus Sporosarcina as Sporosarcina globispora comb. nov., Sporosarcina psychrophila comb. nov and Sporosarcina pasteurii comb. nov., and emended description of the genus Sporosarcina .. Int J Syst Evol Microbiol 511079–1086 [CrossRef]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.64440-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64440-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error