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Abstract

An aerobic bacterium, designated strain E5HC-32, was isolated from soil underlying the decaying leaf litter of a slash pine forest located in south east Queensland, Australia. The strictly aerobic, motile rod-shaped cells (0.8–1.6×2.6–4.8 μm) produced subterminal spherical spores which distended the cells. Strain E5HC-32 grew optimally in 1 % trypticase soy broth (TSB) at 30 °C (temperature range for growth, 25–40 °C) and a pH of 8.4 (pH growth range, pH 7.1–9.1). Electron microscopic examination of negatively stained cells revealed the presence of peritrichous flagella and thin sections showed the presence of a typical Gram-positive type cell-wall ultrastructure. The strain was catalase-positive and oxidase-negative and metabolized pyruvic acid methyl ester, -galactonic acid lactone, -ketobutyric acid, -ketovaleric acid, -proline, -alanine, urocanic acid, inosine, uridine, thymidine, glycerol, -cyclodextrin, --lactose, -psicose, -raffinose, -rhamnose, -sorbitol, turanose, -aconitic acid, -hydroxybutyric acid, -alaninamide and 2-aminoethanol. The G+C content of DNA was 41±1 mol% as determined by the thermal denaturation method. 16S rRNA gene sequence analysis revealed that strain E5HC-32 was placed equidistantly as a member of the class , phylum , with DSM 28 and ATCC PTA-4993 (similarity of 93 %). In addition to its significant phylogenetic separation from its nearest relatives, strain E5HC-32 possessed phenotypic traits that also suggested that it represented a novel species, for which the name sp. nov. is proposed. The type strain is E5HC-32 (=JCM 13601=DSM 17725).

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2007-05-01
2019-10-16
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vol. , part 5, pp. 974 - 978

A transmission electron micrograph of a cell of E5HC-32 . [PDF](279 KB)



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