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Abstract

A Gram-negative, strictly aerobic, non-spore-forming bacterium, motile by means of single polar flagellum and rod-shaped, designated strain GR12-1, was isolated from soil of a ginseng field in Yeongju region, Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that this strain is related to members of the genus , showing sequence similarity values ranged from 92.3 to 96.2 %. This organism grew at 5–33 °C, with optimum growth at 28 °C. Strain GR12-1 grew optimally in the presence of 0–2 % NaCl. The whole-cell fatty acid profile included iso-C, iso-C 9, iso-C, iso-C 3-OH and iso-C as major components. The only isoprenoid quinone was ubiquinone 8 (Q-8). The DNA G+C content was 63.4 mol%. On the basis of phenotypic, genetic and phylogenetic data, strain GR12-1 should be classified as a member of a novel species of the genus , for which the name sp. nov. is proposed, with strain GR12-1 (=KACC 11580=DSM 18204) as the type strain.

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2007-03-01
2019-12-06
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References

  1. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. (editors) ( 1987; ). Current Protocols in Molecular Biology. New York: Greene/Wiley Interscience.
  2. Chang, J. S., Chou, C. L., Lin, G. H., Sheu, S. Y. & Chen, W. M. ( 2005; ). Pseudoxanthomonas kaohsiungensis, sp. nov., a novel bacterium isolated from oil-polluted site produces extracellular surface activity. Syst Appl Microbiol 28, 137–144.[CrossRef]
    [Google Scholar]
  3. Chen, M. Y., Tsay, S. S., Chen, K. Y., Shi, Y. C., Lin, Y. T. & Lin, G. H. ( 2002; ). Pseudoxanthomonas taiwanensis sp. nov., a novel thermophilic, N2O-producing species isolated from hot springs. Int J Syst Evol Microbiol 52, 2155–2161.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  5. Finkmann, W., Altendorf, K., Stackebrandt, E. & Lipski, A. ( 2000; ). Characterization of N2O-producing Xanthomonas-like isolates from biofilters as Stenotrophomonas nitritireducens sp. nov., Luteimonas mephitis gen. nov., sp. nov. and Pseudoxanthomonas broegbernensis gen. nov., sp. nov. Int J Syst Evol Microbiol 50, 273–282.[CrossRef]
    [Google Scholar]
  6. Garrity, G. M. & Holt, J. G. ( 2001; ). The roadmap to the Manual. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 119–166. Edited by G. M. Garrity, D. R. Boone & R. W. Castenholz. New York: Springer.
  7. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  8. Harada, R. M., Campbell, S. & Li, Q. X. ( 2006; ). Pseudoxanthomonas kalamensis sp. nov., a novel gammaproteobacterium isolated from Johnston Atoll, North Pacific Ocean. Int J Syst Evol Microbiol 56, 1103–1107.[CrossRef]
    [Google Scholar]
  9. Hiraishi, A. ( 1992; ). Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett Appl Microbiol 15, 210–213.[CrossRef]
    [Google Scholar]
  10. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  11. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  12. Kwon, S. W., Kim, J. S., Park, I. C., Yoon, S. H., Park, D. H., Lim, C. K. & Go, S. J. ( 2003; ). Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. Int J Syst Evol Microbiol 53, 21–27.[CrossRef]
    [Google Scholar]
  13. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  14. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  15. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note no. 101. Newark, DE: MIDI Inc.
  16. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  17. Thierry, S., Macarie, H., Iizuka, T., Geißdörfer, W., Assih, E. A., Spanevello, M., Verhe, F., Thomas, P., Fudou, R. & other authors ( 2004; ). Pseudoxanthomonas mexicana sp. nov. and Pseudoxanthomonas japonensis sp. nov., isolated from diverse environments, and emended descriptions of the genus Pseudoxanthomonas Finkmann et al. 2000 and of its type species. Int J Syst Evol Microbiol 54, 2245–2255.[CrossRef]
    [Google Scholar]
  18. Weon, H. Y., Kim, B. Y., Kim, J. S., Lee, S. Y., Cho, Y. H., Go, S. J., Hong, S. B., Im, W. T. & Kwon, S. W. ( 2006; ). Pseudoxanthomonas suwonensis sp. nov., isolated from cotton waste composts. Int J Syst Evol Microbiol 56, 659–662.[CrossRef]
    [Google Scholar]
  19. Yang, D.-C., Im, W.-T., Kim, M. K. & Lee, S.-T. ( 2005; ). Pseudoxanthomonas koreensis sp. nov. and Pseudoxanthomonas daejeonensis sp. nov. Int J Syst Evol Microbiol 55, 787–791.[CrossRef]
    [Google Scholar]
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vol. , part 3, pp. 646 - 649

Cellular fatty acid profiles of strain GR12-1 and type strains of the genus . [PDF](27 KB)



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