1887

Abstract

A Gram-negative, strictly aerobic, non-spore-forming bacterium, motile by means of single polar flagellum and rod-shaped, designated strain GR12-1, was isolated from soil of a ginseng field in Yeongju region, Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that this strain is related to members of the genus , showing sequence similarity values ranged from 92.3 to 96.2 %. This organism grew at 5–33 °C, with optimum growth at 28 °C. Strain GR12-1 grew optimally in the presence of 0–2 % NaCl. The whole-cell fatty acid profile included iso-C, iso-C 9, iso-C, iso-C 3-OH and iso-C as major components. The only isoprenoid quinone was ubiquinone 8 (Q-8). The DNA G+C content was 63.4 mol%. On the basis of phenotypic, genetic and phylogenetic data, strain GR12-1 should be classified as a member of a novel species of the genus , for which the name sp. nov. is proposed, with strain GR12-1 (=KACC 11580=DSM 18204) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64427-0
2007-03-01
2024-04-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/3/646.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64427-0&mimeType=html&fmt=ahah

References

  1. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. (editors) 1987; Current Protocols in Molecular Biology . New York: Greene/Wiley Interscience;
  2. Chang J. S., Chou C. L., Lin G. H., Sheu S. Y., Chen W. M. 2005; Pseudoxanthomonas kaohsiungensis , sp. nov., a novel bacterium isolated from oil-polluted site produces extracellular surface activity. Syst Appl Microbiol 28:137–144 [CrossRef]
    [Google Scholar]
  3. Chen M. Y., Tsay S. S., Chen K. Y., Shi Y. C., Lin Y. T., Lin G. H. 2002; Pseudoxanthomonas taiwanensis sp. nov., a novel thermophilic, N2O-producing species isolated from hot springs. Int J Syst Evol Microbiol 52:2155–2161 [CrossRef]
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  5. Finkmann W., Altendorf K., Stackebrandt E., Lipski A. 2000; Characterization of N2O-producing Xanthomonas -like isolates from biofilters as Stenotrophomonas nitritireducens sp.nov., Luteimonas mephitis gen. nov., sp. nov. and Pseudoxanthomonas broegbernensis gen. nov., sp. nov.. Int J Syst Evol Microbiol 50:273–282 [CrossRef]
    [Google Scholar]
  6. Garrity G. M., Holt J. G. 2001; The roadmap to the Manual . In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 1 pp  119–166 Edited by Garrity G. M., Boone D. R., Castenholz R. W. New York: Springer;
    [Google Scholar]
  7. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [CrossRef]
    [Google Scholar]
  8. Harada R. M., Campbell S., Li Q. X. 2006; Pseudoxanthomonas kalamensis sp. nov., a novel gammaproteobacterium isolated from Johnston Atoll, North Pacific Ocean. Int J Syst Evol Microbiol 56:1103–1107 [CrossRef]
    [Google Scholar]
  9. Hiraishi A. 1992; Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification. Lett Appl Microbiol 15:210–213 [CrossRef]
    [Google Scholar]
  10. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  11. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; mega2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245 [CrossRef]
    [Google Scholar]
  12. Kwon S. W., Kim J. S., Park I. C., Yoon S. H., Park D. H., Lim C. K., Go S. J. 2003; Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. . Int J Syst Evol Microbiol 53:21–27 [CrossRef]
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  14. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  15. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids . Technical Note no. 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  16. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  17. Thierry S., Macarie H., Iizuka T., Geißdörfer W., Assih E. A., Spanevello M., Verhe F., Thomas P., Fudou R. other authors 2004; Pseudoxanthomonas mexicana sp. nov. and Pseudoxanthomonas japonensis sp. nov.,isolated from diverse environments, and emended descriptions of the genus Pseudoxanthomonas Finkmann et al. 2000 and of its type species. Int J Syst Evol Microbiol 54:2245–2255 [CrossRef]
    [Google Scholar]
  18. Weon H. Y., Kim B. Y., Kim J. S., Lee S. Y., Cho Y. H., Go S. J., Hong S. B., Im W. T., Kwon S. W. 2006; Pseudoxanthomonas suwonensis sp. nov., isolated from cotton waste composts. Int J Syst Evol Microbiol 56:659–662 [CrossRef]
    [Google Scholar]
  19. Yang D.-C., Im W.-T., Kim M. K., Lee S.-T. 2005; Pseudoxanthomonas koreensis sp. nov. and Pseudoxanthomonas daejeonensis sp. nov.. Int J Syst Evol Microbiol 55:787–791 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64427-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64427-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error