1887

Abstract

A Gram-negative, non-motile, neutrophilic, pleomorphic and extremely halophilic archaeon, strain EJ-57, was isolated from saline Lake Ejinor in Inner Mongolia, China. Strain EJ-57 was able to grow at 25–50 °C, required at least 1.8 M NaCl for growth (optimum at 3.4 M NaCl) and grew over a pH range from 6.0 to 8.5 (optimum at pH 7.0). Hypotonic treatment with less than 1.5 M NaCl caused cell lysis. Analysis of the almost complete 16S rRNA gene sequence indicated that the isolate represented a member of the genus in the family . Strain EJ-57 was most closely related to JCM 10478 (96.2 % sequence similarity), NCIMB 777 (95.9 % sequence similarity), JCM 12890 (95.8 % sequence similarity) and NCIMB 786 (95.5 % sequence similarity). However, DNA–DNA hybridization experiments showed that strain EJ-57 was not related to these species, with levels of DNA–DNA relatedness equal to or below 39 %. The major polar lipids of the isolate were CC and CC derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and the disulfated glycolipid S2-DGA-1. The G+C content of the genomic DNA was 64.7 mol%. Comparative analysis of phenotypic characteristics between strain EJ-57 and recognized species supported the conclusion that EJ-57 represents a novel species within this genus, for which the name sp. nov. is proposed. The type strain is EJ-57 (=CECT 7144=JCM 13890=CGMCC 1.6202).

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2006-11-01
2021-03-07
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