1887

Abstract

A Gram-negative, non-motile, neutrophilic, pleomorphic and extremely halophilic archaeon, strain EJ-57, was isolated from saline Lake Ejinor in Inner Mongolia, China. Strain EJ-57 was able to grow at 25–50 °C, required at least 1.8 M NaCl for growth (optimum at 3.4 M NaCl) and grew over a pH range from 6.0 to 8.5 (optimum at pH 7.0). Hypotonic treatment with less than 1.5 M NaCl caused cell lysis. Analysis of the almost complete 16S rRNA gene sequence indicated that the isolate represented a member of the genus in the family . Strain EJ-57 was most closely related to JCM 10478 (96.2 % sequence similarity), NCIMB 777 (95.9 % sequence similarity), JCM 12890 (95.8 % sequence similarity) and NCIMB 786 (95.5 % sequence similarity). However, DNA–DNA hybridization experiments showed that strain EJ-57 was not related to these species, with levels of DNA–DNA relatedness equal to or below 39 %. The major polar lipids of the isolate were CC and CC derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and the disulfated glycolipid S2-DGA-1. The G+C content of the genomic DNA was 64.7 mol%. Comparative analysis of phenotypic characteristics between strain EJ-57 and recognized species supported the conclusion that EJ-57 represents a novel species within this genus, for which the name sp. nov. is proposed. The type strain is EJ-57 (=CECT 7144=JCM 13890=CGMCC 1.6202).

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2006-11-01
2024-12-07
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References

  1. Arahal D. R., Dewhirst F. E., Paster B. J., Volcani B. E., Ventosa A. 1996; Phylogenetic analyses of some extremely halophilic archaea isolated from Dead Sea water, determined on the basis of their 16S rRNA sequences. Appl Environ Microbiol 62:3779–3786
    [Google Scholar]
  2. De Ley J., Tijtgat R. 1970; Evaluation of membrane filter methods for DNA-DNA hybridization. Antonie van Leeuwenhoek 36:461–474 [CrossRef]
    [Google Scholar]
  3. Grant W. D., Kamekura M., McGenity T. J., Ventosa A. 2001; Order I. Halobacteriales Grant & Larsen 1989b, 495VP (Effective publication: Grant & Larsen 1989a, 2216. In Bergey's Manual of Systematic Bacteriology , 2nd edn. vol 1 pp  294–334 Edited by Boone D. R., Castenholz R. W., Garrity G. M. New York: Springer;
    [Google Scholar]
  4. Gutierrez M. C., Kamekura M., Holmes M. L., Dyall-Smith M. L., Ventosa A. 2002; Taxonomic characterization of Haloferax sp. (“ H. alicantei ”) strain Aa 2.2: description of Haloferax lucentensis sp. nov.. Extremophiles 6479–483 [CrossRef]
    [Google Scholar]
  5. Johnson J. L. 1994; Similarity analysis of DNAs. In Methods for General and Molecular Bacteriology . pp  655–681 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
  6. Kamekura M. 1993; Lipids of extreme halophiles. In The Biology of Halophilic Bacteria pp  135–161 Edited by Vreeland R. H., Hochstein L. I. Boca Raton: CRC Press;
    [Google Scholar]
  7. Lopez-Garcia P., Moreira D., Lopez-Lopez A., Rodríguez-Valera F. 2001; A novel haloarchaeal-related lineage is widely distributed in deep oceanic regions. Environ Microbiol 3:72–78 [CrossRef]
    [Google Scholar]
  8. Ludwig W., Strunk O., Westram R. 29 other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  9. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  10. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  11. McGenity T. J., Gemmell R. T., Grant W. D. 1998; Proposal of a new halobacterial genus Natrinema gen. nov., with two species Natrinema pellirubrum nom. nov. and Natrinema pallidum nom. nov.. Int J Syst Bacteriol 48:1187–1196 [CrossRef]
    [Google Scholar]
  12. Oren A. 2001 The order Halobacteriales . In The Prokaryotes. An Evolving Electronic Resource for the Microbiological Community , 3rd edn. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. New York: Springer; http://141.150.157.117:8080/prokPUB/index.htm
    [Google Scholar]
  13. Oren A., Ventosa A., Grant W. D. 1997; Proposed minimal standards for description of new taxa in the order Halobacteriales . Int J Syst Bacteriol 47:233–238 [CrossRef]
    [Google Scholar]
  14. Owen R. J., Hill L. R. 1979; The estimation of base compositions, base pairing and genome size of bacterial deoxyribonucleic acids. In Identification Methods for Microbiologists , 2nd edn. pp  217–296 Edited by Skinner F. A., Lovelock D. W. London: Academic Press;
    [Google Scholar]
  15. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  16. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  17. Torreblanca M., Rodriguez-Valera F., Juez G., Ventosa A., Kamekura M., Kates M. 1986; Classification of non-alkaliphilic halobacteria based on numerical taxonomy and polar lipid composition and description of Haloarcula gen. nov. and Haloferax gen. nov.. Syst Appl Microbiol 8:89–99 [CrossRef]
    [Google Scholar]
  18. Vandamme P., Pot B., Gillis M., De Vos P., Kersters K., Swings J. 1996; Polyphasic taxonomy, a consensus approach to bacterial classification. Microbiol Rev 60:407–438
    [Google Scholar]
  19. Ventosa A. 2006; Unusual microorganisms from unusual habitats: hypersaline environments.. In Prokaryotic Diversity – Mechanisms and Significance (Society for General Microbiology Symposium, no. 66) pp  223–253 Edited by Logan N. A., Lappin-Scott H. M., Oyston P. C. F. Cambridge: Cambridge University Press;
    [Google Scholar]
  20. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  21. Xin H., Itoh T., Zhou P., Suzuki K.-I., Kamekura M., Nakase Y. 2000; Natrinema versiforme sp. nov., an extremely halophilic archeon from Aibi salt lake, Xinjiang, China. Int J Syst Evol Microbiol 50:1297–1303 [CrossRef]
    [Google Scholar]
  22. Xu X.-W., Ren P.-G., Liu S.-J., Wu M., Zhou P.-J. 2005; Natrinema altunense sp. nov., an extremely halophilic archaeon isolated from a salt lake in Altun Mountain of Xin-Jiang, China. Int J Syst Evol Microbiol 55:1311–1314 [CrossRef]
    [Google Scholar]
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