1887

Abstract

A Gram-negative, non-motile, neutrophilic, pleomorphic and extremely halophilic archaeon, strain EJ-57, was isolated from saline Lake Ejinor in Inner Mongolia, China. Strain EJ-57 was able to grow at 25–50 °C, required at least 1.8 M NaCl for growth (optimum at 3.4 M NaCl) and grew over a pH range from 6.0 to 8.5 (optimum at pH 7.0). Hypotonic treatment with less than 1.5 M NaCl caused cell lysis. Analysis of the almost complete 16S rRNA gene sequence indicated that the isolate represented a member of the genus in the family . Strain EJ-57 was most closely related to JCM 10478 (96.2 % sequence similarity), NCIMB 777 (95.9 % sequence similarity), JCM 12890 (95.8 % sequence similarity) and NCIMB 786 (95.5 % sequence similarity). However, DNA–DNA hybridization experiments showed that strain EJ-57 was not related to these species, with levels of DNA–DNA relatedness equal to or below 39 %. The major polar lipids of the isolate were CC and CC derivatives of phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and the disulfated glycolipid S2-DGA-1. The G+C content of the genomic DNA was 64.7 mol%. Comparative analysis of phenotypic characteristics between strain EJ-57 and recognized species supported the conclusion that EJ-57 represents a novel species within this genus, for which the name sp. nov. is proposed. The type strain is EJ-57 (=CECT 7144=JCM 13890=CGMCC 1.6202).

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2006-11-01
2020-01-27
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References

  1. Arahal, D. R., Dewhirst, F. E., Paster, B. J., Volcani, B. E. & Ventosa, A. ( 1996; ). Phylogenetic analyses of some extremely halophilic archaea isolated from Dead Sea water, determined on the basis of their 16S rRNA sequences. Appl Environ Microbiol 62, 3779–3786.
    [Google Scholar]
  2. De Ley, J. & Tijtgat, R. ( 1970; ). Evaluation of membrane filter methods for DNA-DNA hybridization. Antonie van Leeuwenhoek 36, 461–474.[CrossRef]
    [Google Scholar]
  3. Grant, W. D., Kamekura, M., McGenity, T. J. & Ventosa, A. ( 2001; ). Order I. Halobacteriales Grant & Larsen 1989b , 495VP (Effective publication: Grant & Larsen 1989a , 2216). In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 294–334. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  4. Gutierrez, M. C., Kamekura, M., Holmes, M. L., Dyall-Smith, M. L. & Ventosa, A. ( 2002; ). Taxonomic characterization of Haloferax sp. (“H. alicantei”) strain Aa 2.2: description of Haloferax lucentensis sp. nov. Extremophiles 6, 479–483.[CrossRef]
    [Google Scholar]
  5. Johnson, J. L. ( 1994; ). Similarity analysis of DNAs. In Methods for General and Molecular Bacteriology, pp. 655–681. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  6. Kamekura, M. ( 1993; ). Lipids of extreme halophiles. In The Biology of Halophilic Bacteria, pp. 135–161. Edited by R. H. Vreeland & L. I. Hochstein. Boca Raton: CRC Press.
  7. Lopez-Garcia, P., Moreira, D., Lopez-Lopez, A. & Rodríguez-Valera, F. ( 2001; ). A novel haloarchaeal-related lineage is widely distributed in deep oceanic regions. Environ Microbiol 3, 72–78.[CrossRef]
    [Google Scholar]
  8. Ludwig, W., Strunk, O., Westram, R. & 29 other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  9. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  10. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  11. McGenity, T. J., Gemmell, R. T. & Grant, W. D. ( 1998; ). Proposal of a new halobacterial genus Natrinema gen. nov., with two species Natrinema pellirubrum nom. nov. and Natrinema pallidum nom. nov. Int J Syst Bacteriol 48, 1187–1196.[CrossRef]
    [Google Scholar]
  12. Oren, A. ( 2001; ). The order Halobacteriales. In The Prokaryotes. An Evolving Electronic Resource for the Microbiological Community, 3rd edn. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K.-H. Schleifer & E. Stackebrandt. New York: Springer. http://141.150.157.117:8080/prokPUB/index.htm
  13. Oren, A., Ventosa, A. & Grant, W. D. ( 1997; ). Proposed minimal standards for description of new taxa in the order Halobacteriales. Int J Syst Bacteriol 47, 233–238.[CrossRef]
    [Google Scholar]
  14. Owen, R. J. & Hill, L. R. ( 1979; ). The estimation of base compositions, base pairing and genome size of bacterial deoxyribonucleic acids. In Identification Methods for Microbiologists, 2nd edn, pp. 217–296. Edited by F. A. Skinner & D. W. Lovelock. London: Academic Press.
  15. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  16. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  17. Torreblanca, M., Rodriguez-Valera, F., Juez, G., Ventosa, A., Kamekura, M. & Kates, M. ( 1986; ). Classification of non-alkaliphilic halobacteria based on numerical taxonomy and polar lipid composition and description of Haloarcula gen. nov. and Haloferax gen. nov. Syst Appl Microbiol 8, 89–99.[CrossRef]
    [Google Scholar]
  18. Vandamme, P., Pot, B., Gillis, M., De Vos, P., Kersters, K. & Swings, J. ( 1996; ). Polyphasic taxonomy, a consensus approach to bacterial classification. Microbiol Rev 60, 407–438.
    [Google Scholar]
  19. Ventosa, A. ( 2006; ). Unusual microorganisms from unusual habitats: hypersaline environments. In Prokaryotic Diversity – Mechanisms and Significance (Society for General Microbiology Symposium no. 66), pp. 223–253. Edited by N. A. Logan, H. M. Lappin-Scott & P. C. F. Oyston. Cambridge: Cambridge University Press.
  20. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  21. Xin, H., Itoh, T., Zhou, P., Suzuki, K.-I., Kamekura, M. & Nakase, Y. ( 2000; ). Natrinema versiforme sp. nov., an extremely halophilic archeon from Aibi salt lake, Xinjiang, China. Int J Syst Evol Microbiol 50, 1297–1303.[CrossRef]
    [Google Scholar]
  22. Xu, X.-W., Ren, P.-G., Liu, S.-J., Wu, M. & Zhou, P.-J. ( 2005; ). Natrinema altunense sp. nov., an extremely halophilic archaeon isolated from a salt lake in Altun Mountain of Xin-Jiang, China. Int J Syst Evol Microbiol 55, 1311–1314.[CrossRef]
    [Google Scholar]
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