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Abstract

A motile, pleomorphic, red-pigmented archaeon, strain EJ-52, was isolated from water from Lake Ejinor, a saline lake in Inner Mongolia, China. Analysis of the almost-complete 16S rRNA gene sequence showed that the isolate was phylogenetically related to species of the genus , being most closely related to ATCC 29252 (96.1 % sequence similarity), JCM 8891 (95.9 %), AS 1.3239 (95.2 %), AS 1.3528 (95.2 %) and JCM 9060 (95.1 %). The polar lipids of strain EJ-52 were CC derivatives of phosphatidylglycerol phosphate and phosphatidylglycerol phosphate methyl ester and a sulfated diglycosyl diether. Strain EJ-52 requires at least 2.5 M NaCl for growth and grows optimally at 3.4 M NaCl. The strain grows at 25–50 °C, with optimal growth occurring at 35–45 °C. Mg is not required. The DNA G+C content is 64.2 mol%. On the basis of the data obtained in this study, strain EJ52 represents a novel species, for which the name sp. nov. is proposed. The type strain is EJ-52 (=CECT 7145=JCM 13889=CGMCC 1.6295).

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2006-11-01
2019-12-08
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vol. , part 11, pp. 2559 - 2563

Phase-contrast photomicrograph of cells of EJ-52 . Bar, 10 µm.

S Thin-layer chromatograph of polar lipids extracted from strain EJ-52 and some haloarchaea.

[PDF file of Supplementary Figs S1 and S2](134 KB)



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