1887

Abstract

Two novel strains, Z-9 and Z-17, were isolated from a distilled-spirit-fermenting cellar in Hebei Province, China. The cells were Gram-positive, non-spore-forming, non-motile cocci and occurred in pairs or tetrads. The strains were facultatively anaerobic and produced only lactic acid [(−) and (+) isomers in the ratio 50 : 50] from glucose fermentation. Catalase activity was not present. Both strains were able to grow in 6.5 % ethanol and at pH 3.5, but not in 4 % NaCl. The mean genomic G+C content of the two strains was 39.5±0.5 mol% (39 mol% for the type strain, Z-9). The levels of 16S rRNA gene sequence similarity between the two novel strains and related species of the genus ranged from 98.3 to 98.7 %. The levels of DNA–DNA relatedness between strain Z-9 and the phylogenetically closely related pediococci LMG 11484, LMG 11409, LMG 11486 and LMG 22956 were 14.6, 33, 28.7 and 16.8 %, respectively. On the basis of phenotypic, genotypic and phylogenetic analyses, a novel species, sp. nov., is proposed, with strain Z-9 (=AS 1.3889=LMG 23354) as the type strain.

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2006-10-01
2020-09-23
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References

  1. Back W. 1978; Zur Taxonomie der Gattung Pediococcus . Phanotypische und genotypische Abgrenzung der bisher bekannten Arten sowie Beschtribung einer neuen bierschadlichen Art: Pediococcus inopinatus . Brauwissenschaft 31: 237–250, 312–320 (in German
    [Google Scholar]
  2. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [CrossRef]
    [Google Scholar]
  3. De Man J. C., Rogosa M., Sharpe M. E. 1960; A medium for the cultivation of lactobacilli. J Appl Bacteriol 23:130–135 [CrossRef]
    [Google Scholar]
  4. Dobson C. M., Deneer H., Lee S., Hemmingsen S., Glaze S., Ziola B. 2002; Phylogenetic analysis of the genus Pediococcus , including Pediococcus claussenii sp. nov., a novel lactic acid bacterium isolated from beer. Int J Syst Evol Microbiol 52:2003–2010 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1993 phylip (phylogeny inference package), version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
    [Google Scholar]
  6. Franz C. M., Vancanneyt M., Vandemeulebroecke K., De Wachter M., Cleenwerck I., Hoste B., Schillinger U., Holzepfel W. H., Swings J. 2006; Pediococcus stilesii sp. nov., isolated from maize grains. Int J Syst Evol Microbiol 56:329–333 [CrossRef]
    [Google Scholar]
  7. Garvie E. I. 1986; Genus Pediococcus Claussen 1903, 68AL . In Bergey's Manual of Systematic Bacteriology vol 2 pp  1075–1079 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  8. Gunther H. L., White H. R. 1961; The cultural and physiological characters of the pediococci. J Gen Microbiol 26:185–197 [CrossRef]
    [Google Scholar]
  9. Knorr D. 1998; Technological aspects related to microorganisms in functional foods. Trends Food Sci Technol 9:295–306 [CrossRef]
    [Google Scholar]
  10. Kumar H., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  11. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  12. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [CrossRef]
    [Google Scholar]
  13. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  14. Tannock G. W. 1997; Probiotic properties of lactic-acid bacteria: plenty of scope for fundamental R & D. Trends Biotechnol 15:270–274 [CrossRef]
    [Google Scholar]
  15. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  16. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  17. Zhang B., Tong H. C., Dong X. Z. 2005; Pediococcus cellicola sp. nov., a novel lactic acid coccus isolated from a distilled-spirit-fermenting cellar. Int J Syst Evol Microbiol 55:2167–2170 [CrossRef]
    [Google Scholar]
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