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Abstract

A Gram-positive, non-motile, endospore-forming bacterium, designated Gsoil 1517, was isolated from soil of a ginseng field in Pocheon Province (South Korea) and was characterized in order to determine its taxonomic position, using a polyphasic approach. It was found to rod-shaped and aerobic or facultatively anaerobic. It grew optimally at 30 °C and at pH 6.5–7.0. Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 1517 forms a distinct phylogenetic lineage within the genus , being related to JCM 11201 (96.8 %). The strain showed less than 94.3 % sequence similarity with other species. The G+C content of the genomic DNA was found to be 47.8 mol% and the predominant respiratory quinone was MK-7. The major fatty acids were iso-C (42.4 %), anteiso-C (17.4 %), iso-C (9.7 %) and C (6.0 %). On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 1517 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Gsoil 1517 (=KCTC 13929=CCUG 52470=LMG 23408).

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2006-12-01
2020-01-18
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vol. , part 12, pp. 2861 - 2866

Neighbour-joining tree showing the phylogenetic positions of strain Gsoil 1517 and a wider selection of related species based on 16S rRNA gene sequence analysis. [PDF](18 KB)



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