1887

Abstract

Two novel mesophilic, facultatively anaerobic, selenate-reducing bacteria, designated strains FUT3661 and Asr22-7, were isolated from a sediment sample and the alimentary tract of littleneck clams, respectively. Both sources of the samples were collected from the coast of Tokyo Bay, Japan. Cells were Gram-negative rods and motile by means of a polar flagellum. The strains reduced selenate to elemental selenium (Se) and also reduced iron(III) oxyhydroxide, iron(III) citrate, arsenate, manganese(IV) oxide, elemental sulfur and oxygen and used lactate, pyruvate, yeast extract, tryptone and Casamino acids as electron donors and carbon sources. The strains contained both menaquinone (MK-7) and ubiquinones (Q-7 and Q-8) as isoprenoid quinones. The major fatty acids were C and C 9. The G+C content of the genomic DNA was 58.1 mol% for strain FUT3661 and 57.2 mol% for strain Asr22-7. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strains were related to members of the genus (<94.0 % similarities), although the two novel strains formed a separate lineage. 16S rRNA gene sequence similarity between strains FUT3661 and Asr22-7 was 96 %. On the basis of this polyphasic analysis, it was concluded that strains FUT3661 and Asr22-7 represent two novel species within the genus , for which the names sp. nov. (type strain FUT3661=NBRC 101558=DSM 18154) and sp. nov. (type strain Asr22-7=NBRC 101286=DSM 18153) are proposed.

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2006-11-01
2019-12-14
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