1887

Abstract

Two novel sulfate-reducing bacteria, strains ALDC and Lake, which were able to oxidize n-alkanes, were isolated from a naval oily wastewater-storage facility (VA, USA) and from oilfield production water (OK, USA), respectively. The type strain (ALDC) had a narrow substrate specificity and could grow only with n-alkanes (from C to C), pyruvate, butyrate, hexanoic acid and 4-methyloctanoic acid. Cells of strain ALDC stained Gram-negative and were slightly curved, short rods with oval ends (2.5–3.0×1.0–1.4 μm), often occurring in pairs. Cells tended to form aggregates or large clusters and were non-motile and did not form endospores. Optimum growth occurred between 31 and 37 °C and at pH 6.5–7.2. NaCl was not required for growth, but salt concentrations up to 55 g l could be tolerated. The DNA G+C content was 53.6 mol%. Phylogenetic analysis of the 16S rRNA genes revealed that strains ALDC and Lake were closely related, but not identical (99.9 % similarity). The two strains were not closely related to other known alkane-degrading, sulfate-reducing bacteria or to other genera of the . Therefore, it is proposed that strain ALDC (=JCM 13588=ATCC BAA-1302) represents the type strain of a novel species and genus, with the name gen. nov., sp. nov.

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2006-12-01
2020-09-30
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