1887

Abstract

A Gram-positive, rod-shaped, spore-forming bacterium, Gsoil 3088, was isolated from soil from a ginseng field in Pocheon Province in South Korea and characterized in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity, strain Gsoil 3088 was shown to belong to the family , being related to (96.6 %), (96.3 %), (96.1 %), (96.1 %), (96.1 %) and (96.0 %). The phylogenetic distances from other validly described species within the genus were greater than 4.0 % (i.e. there was less than 96.0 % similarity). The G+C content of the genomic DNA was 52.1 mol%. Phenotypic and chemotaxonomic data (major menaquinone, MK-7; fatty acid profile, iso-C, iso-C and anteiso-C) supported the affiliation of strain Gsoil 3088 to the genus . The results of physiological and biochemical tests allowed strain Gsoil 3088 to be distinguished genotypically and phenotypically from species with validly published names. Strain Gsoil 3088, therefore, represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Gsoil 3088 (=KCTC 13938=LMG 23403).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64382-0
2006-11-01
2020-01-27
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/11/2665.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64382-0&mimeType=html&fmt=ahah

References

  1. Allan, R. N., Lebbe, L., Heyrman, J., De Vos, P., Buchanan, C. J. & Logan, N. A. ( 2005; ). Brevibacillus levickii sp. nov. and Aneurinibacillus terranovensis sp. nov., two novel thermoacidophiles isolated from geothermal soils of northern Victoria Land, Antarctica. Int J Syst Evol Microbiol 55, 1039–1050.[CrossRef]
    [Google Scholar]
  2. Atlas, R. M. ( 1993; ). Handbook of Microbiological Media. Edited by L. C. Parks. Boca Raton, FL: CRC Press.
  3. Buck, J. D. ( 1982; ). Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44, 992–993.
    [Google Scholar]
  4. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  5. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  6. Goto, K., Fujita, R., Kato, Y., Asahara, M. & Yokota, A. ( 2004; ). Reclassification of Brevibacillus brevis strains NCIMB 13288 and DSM 6472 (=NRRL NRS-887) as Aneurinibacillus danicus sp. nov. and Brevibacillus limnophilus sp. nov. Int J Syst Evol Microbiol 54, 419–427.[CrossRef]
    [Google Scholar]
  7. Hall, T. A. ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98.
    [Google Scholar]
  8. Hiraishi, A., Ueda, Y., Ishihara, J. & Mori, T. ( 1996; ). Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42, 457–469.[CrossRef]
    [Google Scholar]
  9. Im, W.-T., Jung, H.-M., Cui, Y.-S., Liu, Q.-M., Zhang, S.-L. & Lee, S.-T. ( 2005; ). Cultivation of the three hundreds of bacterial species from soil of a ginseng field and mining the novel lineage bacteria. In Proceedings of the International Meeting of the Federation of Korean Microbiological Societies, abstract A035, pp. 169. Seoul: Federation of Korean Microbiological Societies.
  10. Kim, M. K., Im, W.-T., Ohta, H., Lee, M. & Lee, S.-T. ( 2005; ). Sphingopyxis granuli sp. nov., a β-glucosidase-producing bacterium in the family Sphingomonadaceae in the α-4 subgroup of the Proteobacteria. J Microbiol 43, 152–157.
    [Google Scholar]
  11. Kimura, M. ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  12. Kouker, G. & Jaeger, K.-E. ( 1987; ). Specific and sensitive plate assay for bacterial lipase. Appl Environ Microbiol 53, 211–213.
    [Google Scholar]
  13. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  14. Logan, N. A., Forsyth, L., Lebbe, L. & 8 other authors ( 2002; ). Polyphasic identification of Bacillus and Brevibacillus strains from clinical, dairy and industrial specimens and proposal of Brevibacillus invocatus sp. nov. Int J Syst Evol Microbiol 52, 953–966.[CrossRef]
    [Google Scholar]
  15. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  16. Migula, W. ( 1900; ). System der Bakterien, vol. 2. Jena: Gustav Fisher.
  17. Moore, D. D. & Dowhan, D. ( 1995; ). Preparation and analysis of DNA. In Current Protocols in Molecular Biology, pp. 2–11. Edited by Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. New York: Wiley.
  18. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  19. Sasser, M. ( 1990; ). Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids. MIDI Technical Note 101. Newark, DE: MIDI.
  20. Shida, O., Takagi, H., Kadowaki, K. & Komagata, K. ( 1996; ). Proposal for two genera, Brevibacillus gen. nov. and Aneurinibacillus gen. nov. Int J Syst Bacteriol 46, 939–946.[CrossRef]
    [Google Scholar]
  21. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  22. Ten, L. N., Im, W.-T., Kim, M.-K., Kang, M.-S. & Lee, S.-T. ( 2004; ). Development of a plate technique for screening of polysaccharide-degrading microorganisms by using a mixture of insoluble chromogenic substrates. J Microbiol Methods 56, 375–382.[CrossRef]
    [Google Scholar]
  23. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  24. Tschech, A. & Pfennig, N. ( 1984; ). Growth yield increase linked to caffeate reduction in Acetobacterium woodii. Arch Microbiol 137, 163–167.[CrossRef]
    [Google Scholar]
  25. Widdel, F. & Bak, F. ( 1992; ). Gram-negative mesophilic sulphate-reducing bacteria. In The Prokaryotes, 2nd edn, pp. 3352–3378. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  26. Widdel, F., Kohring, G. & Mayer, F. ( 1983; ). Studies in dissimilatory sulfate-reducing bacteria that decompose fatty acids. III. Characterization of the filamentous gliding Desulfonema limicola gen. nov., sp. nov. and Desulfonema magnum sp. nov. Arch Microbiol 134, 286–294.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64382-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64382-0
Loading

Data & Media loading...

Supplements

vol. , part 11, pp. 2665 - 2669

In Supplementary Table S1, detailing the cellular fatty acid profile of Gsoil 3088 and other members of the genus , one of the fatty acids is incorrect. Fatty acid iso-C ω10 should be iso-C ω10 . A corrected version of Supplementary Table S1 is now available in place of the original version. [ PDF] 175 KB



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error