Halophilic archaeal strains ABH32T and AX-7T were isolated from Aibi salt lake in Xin-Jiang, China, and were subjected to taxonomic studies. The cells of both strains were rod-shaped, and growth required at least 10 % (w/v) NaCl. The cellular polar lipids were identified as phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and mannose-2,6-disulfate (1→2)-glucose glycerol diether (S2-DGD). The isolates were different in that strain ABH32T did not require magnesium and had strong ability to assimilate sugars for growth, whereas strain AX-7T required magnesium and did not assimilate glucose, sucrose or maltose for growth. Both strain ABH32T and strain AX-7T were most closely related to members of the genera Haloterrigena and Natrinema. On the basis of phenotypic and genotypic characteristics, especially their cellular polar lipids, the two isolates were assigned to the genus Haloterrigena. Moreover, the DNA relatedness values for strains ABH32T and AX-7T with respect to members of the genus Haloterrigena were found to be well below 70 %. Thus, strains ABH32T and AX-7T represent two novel species within the genus Haloterrigena, for which the names Haloterrigena longa sp. nov. (type strain ABH32T=CGMCC 1.5334T=JCM 13562T) and Haloterrigena limicola sp. nov. (type strain AX-7T=CGMCC 1.5333T=JCM 13563T) are proposed.
CuiH.-L.,
YangY.,
TothyD.,
ZhouP.-J.,
LiuS.-J.2006; Biodiversity of halophilic Archaea isolated from two salt lakes in Xin-Jiang region of China. Acta Microbiol Sin 46:171–176
FengJ.,
ZhouP.-J.,
LiuS.-J.,
Warren-RhodesK.2005; Halorubrum alkaliphilum sp. nov., a novel haloalkaliphile isolated from a soda lake in Xin-Jiang, China. Int J Syst Evol Microbiol 55:149–152[CrossRef]
HußV. A. R.,
FestlH.,
SchleiferK. H.1983; Studies on the spectrometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192[CrossRef]
McGenityT. J.,
GemmellR. T.,
GrantW. D.1998; Proposal of a new halobacterial genus Natrinema gen. nov., with two species Natrinema pellirubrum nom.nov. and Natrinema pallidum nom. nov. Int J Syst Bacteriol 48:1187–1196[CrossRef]
OrenA.,
VentosaA.,
GrantW. D.1997; Proposal of minimal standards for the description of new taxa in the order Halobacteriales
. Int J Syst Bacteriol 47:233–238[CrossRef]