1887

Abstract

In a previous study, nitrogen-fixing isolates were recovered from the rhizosphere of maize and from surface-sterilized leaves of sugar cane cultivated in Rio de Janeiro, Brazil. On the basis of 16S rRNA gene sequence similarities, these isolates were identified as belonging to the genus , and whole-cell-protein profiles demonstrated that they are closely related to each other. In the present study, novel isolates were recovered from the roots of different sugar-cane varieties cultivated in diverse geographical regions of Brazil. Twenty-one nitrogen-fixing isolates were analysed using polyphasic taxonomy criteria, including DNA–DNA relatedness, 16S rRNA gene sequence similarities, fatty acid profiles, whole-cell-protein patterns and multilocus enzyme electrophoresis profiles, as well as morphological, physiological and biochemical characterization. The analysis confirmed that these isolates belong to a novel species within the genus , for which the name sp. nov. is proposed. The type strain, SRMrh-20 (=LMG 23149=ATCC BAA-1244), was isolated from the rhizosphere of maize var. Avantis A2345 cultivated in Seropédica, Rio de Janeiro.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64362-0
2006-08-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/8/1931.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64362-0&mimeType=html&fmt=ahah

References

  1. Barrett, C. F. & Parker, M. A. ( 2005; ). Prevalence of Burkholderia sp. odule symbionts on four mimosoid legumes from Barro Colorado Island, Panama. Syst Appl Microbiol 28, 57–65.[CrossRef]
    [Google Scholar]
  2. Barrett, C. F. & Parker, M. A. ( 2006; ). Coexistence of Burkholderia, Cupriavidus, and Rhizobium sp. nodule bacteria on two Mimosa spp. in Costa Rica. Appl Environ Microbiol 72, 1198–1206.[CrossRef]
    [Google Scholar]
  3. Brämer, C. O., Vandamme, P., da Silva, L. F., Gomez, J. G. C. & Steinbüchel, A. ( 2001; ). Polyhydroxyalkanoate-accumulating bacterium isolated from soil of a sugar-cane plantation in Brazil. Int J Syst Evol Microbiol 51, 1709–1713.[CrossRef]
    [Google Scholar]
  4. Burris, R. H. ( 1972; ). Nitrogen fixation assay – methods and techniques. Methods Enzymol 24, 415–431.
    [Google Scholar]
  5. Caballero-Mellado, J., Fuentes-Ramirez, L. E., Reis, V. M. & Martinez-Romero, E. ( 1995; ). Genetic structure of Acetobacter diazotrophicus populations and identification of a new genetically distant group. Appl Environ Microbiol 61, 3008–3013.
    [Google Scholar]
  6. Caballero-Mellado, J., Martínez-Aguilar, L., Paredes-Valdez, G. & Estrada-de los Santos, P. ( 2004; ). Burkholderia unamae sp nov., an N2-fixing rhizospheric and endophytic species. Int J Syst Evol Microbiol 54, 1165–1172.[CrossRef]
    [Google Scholar]
  7. Chen, W. M., de Faria, S. M., Straliotto, R. & 10 other authors ( 2005; ). Proof that Burkholderia strains form effective symbioses with legumes: a study of novel Mimosa-nodulating strains from South America. Appl Environ Microbiol 71, 7461–7471.[CrossRef]
    [Google Scholar]
  8. Coenye, T. & Vandamme, P. ( 2003; ). Diversity and significance of Burkholderia species occupying diverse ecological niches. Environ Microbiol 5, 719–729.[CrossRef]
    [Google Scholar]
  9. Coenye, T., LiPuma, J. J., Henry, D., Hoste, B., Vandemeulebroecke, K., Gillis, M., Speert, D. P. & Vandamme, P. ( 2001a; ). Burkholderia cepacia genomovar VI, a new member of the Burkholderia cepacia complex isolated from cystic fibrosis patients. Int J Syst Evol Microbiol 51, 271–279.
    [Google Scholar]
  10. Coenye, T., Mahenthiralingam, E., Henry, D., LiPuma, J. J., Laevens, S., Gillis, M., Speert, D. P. & Vandamme, P. ( 2001b; ). Burkholderia ambifaria sp. nov., a novel member of the Burkholderia cepacia complex including biocontrol and cystic fibrosis-related isolates. Int J Syst Evol Microbiol 51, 1481–1490.
    [Google Scholar]
  11. Cruz, L. M., de Souza, E. M., Weber, O. B., Baldani, J. I., Döbereiner, J. & Pedrosa, F. de O. ( 2001; ). 16S ribosomal DNA characterization of nitrogen-fixing bacteria isolated from banana (Musa spp.) and pineapple (Ananas comosus (L.) Merril). Appl Environ Microbiol 67, 2375–2379.[CrossRef]
    [Google Scholar]
  12. Estrada-de los Santos, P., Bustillos-Cristales, R. & Caballero-Mellado, J. ( 2001; ). Burkholderia, a genus rich in plant-associated nitrogen fixers with wide environmental and geographic distribution. Appl Environ Microbiol 67, 2790–2798.[CrossRef]
    [Google Scholar]
  13. Gillis, M., Trân Van, V., Bardin, R. & 7 other authors ( 1995; ). Polyphasic taxonomy in the genus Burkholderia leading to an emended description of the genus and proposition of Burkholderia vietnamiensis sp. nov. for N2-fixing isolates from rice in Vietnam. Int J Syst Bacteriol 45, 274–289.[CrossRef]
    [Google Scholar]
  14. Goris, J., De Vos, P., Caballero-Mellado, J., Park, J.-H., Falsen, E., Quensen, J. F., III, Tiedje, J. M. & Vandamme, P. ( 2004; ). Classification of the PCB- and biphenyl-degrading strain LB400 and relatives as Burkholderia xenovorans sp. nov. Int J Syst Evol Microbiol 54, 1677–1681.[CrossRef]
    [Google Scholar]
  15. Henry, D. A., Campbell, M. E., LiPuma, J. J. & Speert, D. P. ( 1997; ). Identification of Burkholderia cepacia isolates from patients with cystic fibrosis and use of a simple new selective medium. J Clin Microbiol 35, 614–619.
    [Google Scholar]
  16. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  17. Kumar, S., Tamura, K. & Nei, M. ( 1993; ). mega: molecular evolutionary genetics analysis, version 1.0. University Park, PA: The Pennsylvania State University.
  18. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Tempe, AZ: Arizona State University.
  19. Martínez-Romero, E. & Caballero-Mellado, J. ( 1996; ). Rhizobium phylogenies and bacterial genetic diversity. Crit Rev Plant Sci 15, 113–140.[CrossRef]
    [Google Scholar]
  20. Moulin, L., Munive, A., Dreyfus, B. & Boivin-Masson, C. ( 2001; ). Nodulation of legumes by members of the beta-subclass of Proteobacteria. Nature 411, 948–950.[CrossRef]
    [Google Scholar]
  21. Perin, L., Martínez-Aguilar, L., Castro-González, R., Estrada-de los Santos, P., Cabellos-Avelar, T., Guedes, H. V., Reis, V. M. & Caballero-Mellado, J. ( 2006; ). Diazotrophic Burkholderia species associated with field-grown maize and sugarcane. Appl Environ Microbiol 72, 3103–3110.[CrossRef]
    [Google Scholar]
  22. Poly, F., Jocteur-Monrozier, L. & Bally, R. ( 2001; ). Improvement in the RFLP procedure for studying the diversity of nifH genes in communities of nitrogen fixers in soil. Res Microbiol 152, 95–103.[CrossRef]
    [Google Scholar]
  23. Reis, V. M., Estrada-de los Santos, P., Tenorio-Salgado, S. & 10 other authors ( 2004; ). Burkholderia tropica sp. nov., a novel nitrogen-fixing, plant-associated bacterium. Int J Syst Evol Microbiol 54, 2155–2162.[CrossRef]
    [Google Scholar]
  24. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  25. Stackebrandt, E., Frederiksen, W., Garrity, G. M. & 10 other authors ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52, 1043–1047.[CrossRef]
    [Google Scholar]
  26. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  27. Valdés, M., Pérez, N. O., Estrada-de los Santos, P., Caballero-Mellado, J., Peña-Cabriales, J. J., Normand, P. & Hirsch, A. M. ( 2005; ). Non-Frankia actinomycetes isolated from surface-sterilized roots of Casuarina equisetifolia fix nitrogen. Appl Environ Microbiol 71, 460–466.[CrossRef]
    [Google Scholar]
  28. Vandamme, P., Pot, B., Gillis, M., de Vos, P., Kersters, K. & Swings, J. ( 1996; ). Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 60, 407–438.
    [Google Scholar]
  29. Vandamme, P., Holmes, B., Vancanneyt, M. & 8 other authors ( 1997; ). Occurrence of multiple genomovars of Burkholderia cepacia in cystic fibrosis patients and proposal of Burkholderia multivorans sp. nov. Int J Syst Bacteriol 47, 1188–1200.[CrossRef]
    [Google Scholar]
  30. Vandamme, P., Goris, J., Chen, W.-M., de Vos, P. & Willems, A. ( 2002; ). Burkholderia tuberum sp. nov. and Burkholderia phymatum sp. nov., nodulate the roots of tropical legumes. Syst Appl Microbiol 25, 507–512.[CrossRef]
    [Google Scholar]
  31. Yabuuchi, E., Kosako, Y., Oyaizu, H., Yano, I., Hotta, H., Hashimoto, Y., Ezaki, T. & Arakawa, M. ( 1992; ). Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov. Microbiol Immunol 36, 1251–1275.[CrossRef]
    [Google Scholar]
  32. Zhang, H., Hanada, S., Shigematsu, T., Shibuya, K., Kamagata, Y., Kanagawa, T. & Kurane, R. ( 2000; ). Burkholderia kururiensis sp. nov., a trichloroethylene (TCE)-degrading bacterium isolated from an aquifer polluted with TCE. Int J Syst Evol Microbiol 50, 743–749.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64362-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64362-0
Loading

Data & Media loading...

Supplements

vol. , part 8, pp. 1931 - 1937

Relationships among N -fixing isolates and reference N -fixing species and related non-diazotrophic species as determined by MLEE.

Whole-cell protein profiles (SDS-PAGE) of representative strains of sp. nov. and type strains of related species.

[PDF file of Supplementary Figs S1 and S2](319 KB)



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error