1887

Abstract

Two Gram-positive, aerobic, spore-forming rods, F73 and I80, were isolated from upland soil. A phylogenetic analysis of 16S rRNA gene sequences placed both isolates within the genus , and showed a sequence similarity of 98.9 % between the two strains and a similarity of approximately 94.6–97.3 % with respect to species with validly published names. The values for DNA–DNA relatedness between the two isolates and related type strains of the genus were below 28.0 %. For both strains, the major cellular fatty acids were anteiso-C and iso-C. In both cases, the cell-wall peptidoglycan was of the A4 type (-Lys–-Glu) and the major menaquinone was MK-7. Diaminopimelic acid was absent from both strains. The genomic DNA G+C contents of strains F73 and I80 were 46.5 and 44.5 mol%, respectively. On the basis of the phylogenetic analysis and physiological and chemotaxonomic data, the isolates represent two novel species of the genus , for which the names sp. nov. (type strain F73 =KACC 11299 =DSM 16921) and sp. nov. (type strain I80 =KACC 11300 =DSM 16920) are proposed.

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2007-08-01
2019-10-17
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Two-dimensional thin-layer chromatograms of polar lipids from strains F73 (left) and I80 (right). DPG, Diphosphatidylglycerol; N1, unidentified aminolipid; P1, P2, unidentified phospholipids; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PN1, PN2, unidentified aminophospholipids.

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Two-dimensional thin-layer chromatograms of polar lipids from strains F73 (left) and I80 (right). DPG, Diphosphatidylglycerol; N1, unidentified aminolipid; P1, P2, unidentified phospholipids; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PN1, PN2, unidentified aminophospholipids.

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