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Abstract

Psychrotolerant (0–25 °C), chemolithotrophic Gram-negative cocci were isolated from Alaskan forest soil. The novel isolate was found to grow autotrophically on H : CO mixtures and to switch to heterotrophic growth on media containing organic substrates. The novel strain utilized a wide range of organic acids, some simple sugars and alcohols. Naphthalene vapour did not support growth. On the basis of 16S rRNA gene sequence similarity, the novel strain is affiliated to the genus , of the class , and is related to (99.6 % gene sequence similarity), (97.4 %) and (96.1 %). The membrane phospholipids contained 16 : 17/16 : 16, 16 : 0 and 18 : 17, similar to the fatty acids found for , and . On the basis of DNA–DNA hybridization, physiological and biochemical properties, the hydrogen-oxidizing mixotrophic isolate represents a novel species, for which the name sp. nov. is proposed. The type strain is DSM 17735 (=NRRL B-41369).

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2007-03-01
2020-01-17
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A scanning electron microscope image of cells of strain DSM 17735 and phylogenetic trees based on analysis of 16S rRNA gene sequences constructed by the neighbour-joining and maximum-likelihood methods. [PDF](193 KB)

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Fatty acid contents of strain DSM 17735 and closely related species of the genus . [PDF](56 KB)

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