1887

Abstract

A yellow-pigmented, Gram-negative, rod-shaped, strictly aerobic bacterium (strain UST050418-085) was isolated from the surface of a marine sponge, , at Friday Harbor, WA, USA. The DNA G+C content of this strain was 34.6 mol%. The predominant fatty acids were i15 : 0, a15 : 0, i15 : 1, i16 : 0, i17 : 0 3-OH, 17 : 0 2-OH and summed feature 3, comprising i15 : 0 2-OH and/or 16 : 17 (altogether representing 69.0 % of the total fatty acids). MK-6 was the only respiratory quinone detected. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the closest relatives of UST050418-085 were members of the genus , with sequence similarities of 93.2–96.6 %. Strain UST050418-085 differed from its closest relatives by 11 to 18 phenotypic traits. Molecular evidence and phenotypic characteristics suggest that strain UST050418-085 represents a novel species within the genus . The name sp. nov. is proposed, with UST050418-085 (=JCM 13546=NRRL B-41416) as the type strain.

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2006-08-01
2024-03-29
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References

  1. Acar J. F. 1980; The disk susceptibility test. In Antibiotics in Laboratory and Medicine pp  24–54 Edited by Lorian V. Baltimore: Williams & Wilkins;
    [Google Scholar]
  2. Baumann P., Baumann L. 1981; The marine gram-negative eubacteria: genera Photobacterium Beneckea , Alteromonas , Pseudomonas and Alcaligenes . In The Prokaryotes , vol. 1 pp  1302–1331 Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. Berlin: Springer;
    [Google Scholar]
  3. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868
    [Google Scholar]
  4. Bowman J. P. 2004; Psychrophilic prokaryote structural-functional relationships, biogeography and evolution within marine sediment. Cell Mol Biol 50:503–515
    [Google Scholar]
  5. Bowman J. P., Nichols D. S. 2005; Novel members of the family Flavobacteriaceae from Antarctic maritime habitats including Subsaximicrobium wynnwilliamsii gen. nov., sp. nov., Subsaximicrobium saxinquilinus sp.nov., Subsaxibacter broadyi gen. nov., sp. nov., Lacinutrix copepodicola gen. nov., sp. nov., and novel species of the genera Bizionia , Gelidibacter and Gillisia . Int J Syst Evol Microbiol 55, 1471–1486 [CrossRef]
  6. Collins M. D. 1994; Isoprenoid quinones. In Chemical Methods in Prokaryotic Systematics pp  265–310 Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  7. Isnansetyo A., Kamei Y. 2003; Pseudoalteromonas phenolica sp. nov., a novel marine bacterium that produces phenolic anti-methicillin-resistant Staphylococcus aureus substances. Int J Syst Evol Microbiol 53:583–588 [CrossRef]
    [Google Scholar]
  8. Lau S. C. K., Tsoi M. M. Y., Li X., Plakhotnikova I., Wu M., Wong P. K., Qian P. Y. 2004; Loktanella hongkongensis sp. nov., a novel member of the α-Proteobacteria originating from marine biofilms in Hong Kong waters. Int J Syst Evol Microbiol 54:2281–2284 [CrossRef]
    [Google Scholar]
  9. Lau S. C. K., Tsoi M. M. Y., Li X. 7 other authors 2005; Winogradskyella poriferorum sp. nov., a novel member of the family Flavobacteriaceae isolated from a sponge in the Bahamas. Int J Syst Evol Microbiol 55:1589–1592 [CrossRef]
    [Google Scholar]
  10. Ludwig W., Strunk O., Westram R. 29 other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  11. MacDonell M. T., Singleton F. L., Hood M. A. 1982; Diluent composition for use of API 20E in characterizing marine and estuarine bacteria. Appl Environ Microbiol 44:423–427
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  13. Nakagawa Y., Yamasato K. 1993; Phylogenetic diversity of the genus Cytophaga revealed by 16S rRNA sequencing and menaquinone analysis. J Gen Microbiol 139:1155–1161 [CrossRef]
    [Google Scholar]
  14. Nedashkovskaya O. I., Kim S. B., Hans S. K. 7 other authors 2003 Mesonia algae gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae isolated from the green alga Acrosiphonia sonderi (Kütz) Kornm. Int J Syst Evol Microbiol 531967–1971 [CrossRef]
  15. Nedashkovskaya O. I., Kim S. B., Lee K. H., Mikhailov V. V., Bae K. S. 2005; Gillisia mitskevichiae sp. nov., a novel bacterium of the family Flavobacteriaceae , isolated from sea water. Int J Syst Evol Microbiol 55:321–323 [CrossRef]
    [Google Scholar]
  16. Neu B., Voigt A., Mitlohner R. 7 other authors 2001; Biological cells as templates for hollow microcapsules. J Microencapsul 18:385–395 [CrossRef]
    [Google Scholar]
  17. Norris J. R., Ribbons D. W., Varma A. K. (editors) 1985 Methods in Microbiology , vol. 18 London: Academic Press;
    [Google Scholar]
  18. Smibert R. M., Krieg N. R. 1994; Phenotypic characteristics. In Methods for General and Molecular Biology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  19. Steyn P. L., Segers P., Vancanneyt M., Sandra P., Kersters K., Joubert J. J. 1998; Classification of heparinolytic bacteria into a new genus, Pedobacter , comprising four species: Pedobacter heparinus comb.nov., Pedobacter piscium comb. nov., Pedobacter africanus sp. nov. and Pedobacter saltans sp. nov.Proposal of the family Sphingobacteriaceae . Int J Syst Bacteriol 48:165–177 [CrossRef]
    [Google Scholar]
  20. Van Trappen S., Vandecandelaere I., Mergaert J., Swings J. 2004; Gillisia limnaea gen. nov., sp. nov., a new member of the family Flavobacteriaceae isolated from a microbial mat in Lake Fryxell, Antarctica. Int J Syst Evol Microbiol 54:445–448 [CrossRef]
    [Google Scholar]
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