1887

Abstract

Three strains, 002-048, RB589 and 002-051, isolated from field soil in Japan, were characterized using a polyphasic approach. The isolates were Gram-positive, strictly aerobic, non-motile rods that formed ellipsoidal, subterminal endospores. The chemotaxonomic characteristics of these isolates included the presence of -diaminopimelic acid as the cell-wall peptidoglycan, anteiso-C and anteiso-C as the major cellular fatty acids and MK-7 as the predominant menaquinone. The DNA G+C content was 44–46 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the isolates represented an independent lineage that is distinct from related taxa and exhibited less than 94.3 % sequence similarity with respect to those taxa. Moreover, a DNA–DNA hybridization analysis showed that the three isolates represented two species. On the basis of their phenotypic and phylogenetic distinctiveness, the isolates represent two species within a novel genus, for which the names gen. nov., sp. nov. and sp. nov. are proposed. The type strain of is 002-048 (=IAM 15309=KCTC 13936) and the type strain of is 002-051 (=IAM 15310=KCTC 13937).

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2007-01-01
2021-04-14
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