1887

Abstract

A marine bacterial strain, FR1064, was isolated from a surface seawater sample collected off Jeju Island, South Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate belonged to the and was related to the genus with 97.6 % sequence similarity to , its nearest phylogenetic neighbour. DNA–DNA relatedness between strain FR1064 and ACAM 615 was 55 %. Cells of the novel isolate were Gram-negative, aerobic, rod-shaped, motile and halophilic, with an optimum sea salts concentration of 4–7 %. The major fatty acids were straight-chain saturated (C), summed feature 3 and monounsaturated fatty acid C. The DNA G+C content was 44 mol%. Several phenotypic characteristics differentiated the novel isolate from all previously described members of the genus . The polyphasic data obtained in this study clearly demonstrate that strain FR1064 represents a novel species of the genus . The name sp. nov. is therefore proposed, with strain FR1064 (=KCTC 12276=JCM 12485) as the type strain.

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2006-09-01
2019-12-06
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References

  1. Bowman, J. P., McCammon, S. A., Brown, J. L. & McMeekin, T. A. ( 1998; ). Glaciecola punicea gen. nov., sp. nov. and Glaciecola pallidula gen. nov., sp. nov.: psychrophilic bacteria from Antarctic sea-ice habitats. Int J Syst Bacteriol 48, 1213–1222.[CrossRef]
    [Google Scholar]
  2. Chun, J. & Goodfellow, M. ( 1995; ). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. Int J Syst Bacteriol 45, 240–245.[CrossRef]
    [Google Scholar]
  3. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  5. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  6. Fitch, W. M. & Margoliash, E. ( 1967; ). Construction of phylogenetic trees. Science 155, 279–284.[CrossRef]
    [Google Scholar]
  7. Gutell, R. R. ( 1994; ). Collection of small subunit (16S- and 16S-like) ribosomal RNA structures: 1994. Nucleic Acids Res 22, 3502–3507.[CrossRef]
    [Google Scholar]
  8. Jeon, Y.-S., Chung, H., Park, S., Hur, I., Lee, J.-H. & Chun, J. ( 2005; ). jphydit: a java-based integrated environment for molecular phylogeny of ribosomal RNA sequences. Bioinformatics 21, 3171–3173.[CrossRef]
    [Google Scholar]
  9. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  10. Kim, B. S., Oh, H. M., Kang, H. & Chun, J. ( 2005; ). Archaeal diversity in tidal flat sediment as revealed by 16S rDNA analysis. J Microbiol 43, 144–151.
    [Google Scholar]
  11. Kovacs, N. ( 1956; ). Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 178, 703.
    [Google Scholar]
  12. Lyman, J. & Fleming, R. H. ( 1940; ). Composition of sea water. J Mar Res 3, 134–146.
    [Google Scholar]
  13. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  14. Romanenko, L. A., Zhukova, N. V., Rohde, M., Lysenko, A. M., Mikhailov, V. V. & Stackebrandt, E. ( 2003; ). Glaciecola mesophila sp. nov., a novel marine agar-digesting bacterium. Int J Syst Evol Microbiol 53, 647–651.[CrossRef]
    [Google Scholar]
  15. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  16. Seldin, L. & Dubnau, D. ( 1985; ). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35, 151–154.[CrossRef]
    [Google Scholar]
  17. Swofford, D. L. ( 1998; ). paup*: Phylogenetic analysis using parsimony (*and other methods), version 4. Sunderland, MA: Sinauer Associates.
  18. Van Trappen, S. V., Tan, T. L., Yang, J., Mergaert, J. & Swings, J. ( 2004; ). Glaciecola polaris sp. nov., a novel budding and prosthecate bacterium from the Arctic Ocean, and emended description of the genus Glaciecola. Int J Syst Evol Microbiol 54, 1765–1771.[CrossRef]
    [Google Scholar]
  19. Wayne, L. G., Brenner, D. J., Colwell, R. R. & 9 other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  20. Yi, H. & Chun, J. ( 2006; ). Thalassobius aestuarii sp. nov., isolated from tidal flat sediment. J Microbiol 44, 171–176.
    [Google Scholar]
  21. Zobell, C. E. ( 1941; ). Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 4, 42–75.
    [Google Scholar]
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