1887

Abstract

The taxonomic positions of five bacterial strains isolated from the Yaeyama Islands of Japan and ‘’ NBRC 15982 were determined using a polyphasic taxonomic approach. 16S rRNA gene sequence analyses placed all of the strains close to the genus . DNA–DNA hybridization studies, biochemical and physiological characterizations and chemotaxonomic analyses suggested that ‘’ NBRC 15982 and the five novel isolates represented two separate species of the genus . Emendation of the genus Nakagawa . 1997 and the species (Reichenbach 1989) Nakagawa . 1997 is proposed. In addition, ‘ Lewin 1969 is proposed as nom. rev., comb. nov. (with the type strain NBRC 15982=CIP 109101) and the novel isolates are proposed as sp. nov. (type strain IR25-3=NBRC 100898=CIP 109099).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64324-0
2006-09-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/9/2095.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64324-0&mimeType=html&fmt=ahah

References

  1. Barrow G. I., Feltham R. K. A. 1993 Cowan and Steel's Manual for the Identification of Medical Bacteria Cambridge: Cambridge University Press;
    [Google Scholar]
  2. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. 1978; Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci U S A 75:4801–4805 [CrossRef]
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid- deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  4. Fautz E., Reichenbach H. 1980; A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8:87–91 [CrossRef]
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  6. Gutell R. R., Larsen N., Woese C. R. 1994; Lessons from an evolving rRNA: 16S and 23S rRNA structures from a comparative perspective. Microbiol Rev 58:10–26
    [Google Scholar]
  7. Hamana K., Nakagawa Y., Yamasato K. 1995; Chemotaxonomic significance of polyamine distribution patterns in the Flavobacterium-Cytophaga complex and related genera. Microbios 81:135–145
    [Google Scholar]
  8. Hosoya R., Hamana K. 2003; Absence of cellular triamines in four novel flavobacteria located in Flavobacterium–Flexibacter–Cytophaga complex. Ann Gunma Health Sci 24:13–16
    [Google Scholar]
  9. Hugh R., Leifson E. 1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram negative bacteria. J Bacteriol 66:24–26
    [Google Scholar]
  10. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  11. Lau K. W. K., Ng C. Y. M., Ren J., Lau S. C. L., Qian P.-Y., Wong P.-K., Lau T. C., Wu M. 2005; Owenweeksia hongkongensis gen. nov., sp. nov., a novel marine bacterium of the phylum ‘ Bacteroidetes ’. Int J Syst Evol Microbiol 55, 1051–1057 [CrossRef]
  12. Lewin R. A. 1969; A classification of flexibacteria. J Gen Microbiol 58:189–206 [CrossRef]
    [Google Scholar]
  13. Lewin R. A., Lounsbery D. M. 1969; Isolation, cultivation and characterization of flexibacteria. J Gen Microbiol 58:145–170 [CrossRef]
    [Google Scholar]
  14. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  15. Nakagawa Y. 2004; Taxonomic studies of Cytophaga -like bacteria. Microbiol Cult Coll 20:41–51
    [Google Scholar]
  16. Nakagawa Y., Yamasato K. 1993; Phylogenetic diversity of the genus Cytophaga revealed by 16S rRNA sequencing and menaquinone analysis. J Gen Microbiol 139:1155–1161 [CrossRef]
    [Google Scholar]
  17. Nakagawa Y., Hamana K., Sakane T., Yamasato K. 1997 Reclassification of Cytophaga aprica (Lewin 1969) Reichenbach 1989 in Flammeovirga gen. nov. as Flammeovirga aprica comb.nov. and of Cytophaga diffluens ( ex Stanier 1940; emend. Lewin 1969) Reichenbach 1989 in Persicobacter gen. nov. as Persicobacter diffluens comb. nov. Int J Syst Bacteriol 47, 220–223 [CrossRef]
  18. Nakagawa Y., Sakane T., Suzuki M., Hatano K. 2002; Phylogenetic structure of the genera Flexibacter , Flexithrix , and Microscilla deduced from 16S rRNA sequence analysis. J Gen Appl Microbiol 48:155–165 [CrossRef]
    [Google Scholar]
  19. Perry L. B. 1973; Gliding motility in some non-spreading flexibacteria. J Appl Microbiol 36:227–232
    [Google Scholar]
  20. Reichenbach H. 1989a; Genus I Cytophaga Winogradsky 1929, 577AL, emend. In Bergey's Manual of Systematic Bacteriology vol  3 pp  2015–2050 Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  21. Reichenbach H. 1989b; Genus Microscilla Pringsheim 1951, 140, emend. Lewin 1969, 194AL. In Bergey's Manual of Systematic Bacteriology , vol. 3 pp  2071–2073 Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  22. Rüger H.-J., Krambeck H.-J. 1994; Evaluation of the BIOLOG substrate metabolism system for classification of marine bacteria. Syst Appl Microbiol 17:281–288 [CrossRef]
    [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  24. Schmidt K., Connor A., Britton G. 1994; Analysis of pigments: carotenoids and related polyenes. In Chemical Methods in Prokaryotic Systematics pp  403–461 Edited by Goodfellow M., O'Donnell A. G. West Sussex: Wiley;
    [Google Scholar]
  25. Smibert R. M., Krieg N. R. 1981; General characterization. In Manual of Methods for General Bacteriology pp  409–443 Edited by Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  26. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. 2001; Phylogenetic analysis and taxonomic study of marine Cytophaga -like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb.nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp.nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 51:1639–1652 [CrossRef]
    [Google Scholar]
  27. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64324-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64324-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error