A novel moderately halophilic, sulfate-reducing bacterium, strain EtOH3T, was isolated from anoxic hypersaline (270 g NaCl l−1) sediment of the northern arm of the Great Salt Lake, Utah, USA. Cells of strain EtOH3T were oval to rod-shaped, non-motile, non-sporulating and stained Gram-negative. The strain required sodium and magnesium ions for growth and grew at salinities of up to 240 g NaCl l−1 and 121 g MgCl2.6H2O l−1. The optimum NaCl concentration was 80–100 g l−1. Strain EtOH3T grew at temperatures ranging from 15 to 44 °C (optimum 37 °C). The pH range for growth was 6.5–8.3 (optimum around pH 6.8). Only sulfate and thiosulfate served as electron acceptors for a broad range of electron donors including various short-chain fatty acids and primary (C1–5) alcohols, amino acids, H2/acetate and H2/yeast extract. The G+C content of the genomic DNA was 51.4 mol%. Phylogenetic analysis of dsrAB [genes encoding the major subunits of dissimilatory (bi)sulfite reductase] and 16S rRNA gene sequence data placed strain EtOH3T within the deltaproteobacterial family Desulfohalobiaceae. Strain EtOH3T shared 76 and 91 % dsrAB and 16S rRNA gene sequence similarity, respectively, with the type strain of the phylogenetically most closely related species with a validly published name, Desulfohalobium retbaense DSM 5692T. High 16S rRNA gene sequence similarity (~97 %) was shared with the recently described strain ‘Desulfovermiculus halophilus’ VKM B-2364. Strain EtOH3T, however, clearly differed from this strain in both genomic G+C content and in several of its phenotypic properties. On the basis of phenotypic and genotypic characteristics, the novel species Desulfohalobium utahense sp. nov. is proposed, with strain EtOH3T (=VKM B-2384T=DSM 17720T) as the type strain.
BelyakovaE. V.,
RozanovaE. P.,
BorzenkovI. A.,
TourovaT. P.,
PushevaM. A.,
LysenkoA. M.,
KolganovaT. V.2006 The new facultatively chemolithoautotrophic, moderately halophilic, sulfate-reducing bacterium Desulfovermiculus halophilus gen.nov., sp. nov., isolated from an oil field. Microbiology (English translation of Mikrobiologiia ) 75161–171[CrossRef]
BrandtK. K.,
IngvorsenK.1997; Desulfobacter halotolerans sp. nov., a halotolerant acetate-oxidizing sulfate-reducing bacterium isolated from sediments of Great Salt Lake, Utah. Syst Appl Microbiol 20:366–373[CrossRef]
BrandtK. K.,
VesterF.,
JensenA. N.,
IngvorsenK.2001; Sulfate reduction dynamics and enumeration of sulfate-reducing bacteria in hypersaline sediments of the Great Salt Lake. (Utah, USA: Microb Ecol 41:1–11
ElsgaardL.,
IsaksenM. F.,
JørgensenB. B.,
AlayseA.-M.,
JannaschH. W.1994; Microbial sulfate reduction in deep-sea sediments at the Guaymas Basin hydrothermal vent area: influence of temperature and substrates. Geochim Cosmochim Acta 58:3335–3343[CrossRef]
KrekelerD.,
SigalevichP.,
TeskeA.,
CypionkaH.,
CohenY.1997; A sulfate-reducing bacterium from the oxic layer of a microbial mat from Solar Lake (Sinai), Desulfovibrio oxyclinae sp. nov. Arch Microbiol 167:369–375[CrossRef]
KueverJ.,
RaineyF. A.,
WiddelF.2005 Family III. Desulfohalobiaceae fam. nov. In Bergey's Manual of Systematic Bacteriology , 2nd edn, vol. 2 ( The Proteobacteria ), part C ( The Alpha -, Beta -, Delta -, and Epsilonproteobacteria ) pp 948–952 Edited by
BrennerD. J.,
KriegN. R.,
StaleyJ. T.,
GarrityG. M.
New York: Springer;
KueverJ.,
RaineyF. A.,
WiddelF.2006; Desulfothermus gen. nov. In List of New Names and Combinations Previously Effectively, but not Validly, Published , Validation List no. 107. Int J Syst Evol Microbiol 56:1–6[CrossRef]
OllivierB.,
HatchikianC. E.,
PrensierG.,
GuezennecJ.,
GarciaJ.-L.1991; Desulfohalobium retbaense gen. nov., sp. nov., a halophilic sulfate-reducing bacterium from sediments of a hypersaline lake in Senegal. Int J Syst Bacteriol 41:74–81[CrossRef]
SassH.,
CypionkaH.2004; Isolation of sulfate-reducing bacteria from the terrestrial deep subsurface and description of Desulfovibrio cavernae sp. nov. Syst Appl Microbiol 27:541–548[CrossRef]
SievertS. M.,
KueverJ.2000; Desulfacinum hydrothermale sp. nov., a thermophilic, sulfate-reducing bacterium from geothermally heated sediments near Milos Island (Greece). Int J Syst Evol Microbiol 50:1239–1246[CrossRef]
WelshD. T.,
LindsayY. E.,
CaumetteP.,
HerbertR. A.,
HannanJ.1996; Identification of trehalose and glycine betaine as compatible solutes in the moderately halophilic sulfate reducing bacterium, Desulfovibrio halophilus
. FEMS Microbiol Lett 140:203–207[CrossRef]
ZhilinaT. N.,
ZavarzinG. A.,
RaineyF. A.,
PikutaE. N.,
OsipovG. A.,
KostrikinaN. A.1997; Desulfonatronovibrio hydrogenovorans gen. nov., sp. nov., an alkaliphilic sulfate-reducing bacterium.. Int J Syst Bacteriol 47:144–149[CrossRef]