1887

Abstract

A novel moderately halophilic, sulfate-reducing bacterium, strain EtOH3, was isolated from anoxic hypersaline (270 g NaCl l) sediment of the northern arm of the Great Salt Lake, Utah, USA. Cells of strain EtOH3 were oval to rod-shaped, non-motile, non-sporulating and stained Gram-negative. The strain required sodium and magnesium ions for growth and grew at salinities of up to 240 g NaCl l and 121 g MgCl.6HO l. The optimum NaCl concentration was 80–100 g l. Strain EtOH3 grew at temperatures ranging from 15 to 44 °C (optimum 37 °C). The pH range for growth was 6.5–8.3 (optimum around pH 6.8). Only sulfate and thiosulfate served as electron acceptors for a broad range of electron donors including various short-chain fatty acids and primary (C) alcohols, amino acids, H/acetate and H/yeast extract. The G+C content of the genomic DNA was 51.4 mol%. Phylogenetic analysis of [genes encoding the major subunits of dissimilatory (bi)sulfite reductase] and 16S rRNA gene sequence data placed strain EtOH3 within the deltaproteobacterial family . Strain EtOH3 shared 76 and 91 % and 16S rRNA gene sequence similarity, respectively, with the type strain of the phylogenetically most closely related species with a validly published name, DSM 5692. High 16S rRNA gene sequence similarity (∼97 %) was shared with the recently described strain ‘’ VKM B-2364. Strain EtOH3, however, clearly differed from this strain in both genomic G+C content and in several of its phenotypic properties. On the basis of phenotypic and genotypic characteristics, the novel species sp. nov. is proposed, with strain EtOH3 (=VKM B-2384=DSM 17720) as the type strain.

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2006-09-01
2019-10-19
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vol. , part 9, pp. 2063 - 2069

Transmission electron micrograph of an uranyl acetate-stained cell of strain EtOH3 . Bar, 1.0 µm.



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