1887

Abstract

A novel Gram-positive bacterium, strain A35, was isolated from coastal soil at Chiba, Japan, and was identified as a member of the genus on the basis of phenotypic and phylogenetic analyses. The bacterium was found to be a facultatively anaerobic and endospore-forming rod. The predominant menaquinone was MK-7, the major cellular fatty acid was anteiso-C and the DNA G+C content was 48.1 mol%. The levels of 16S rRNA gene sequence similarity between strain A35 and species with validly published names were less than 94 %. Strain A35 was clearly distinguishable from reference species for the genus . Therefore, on the basis of these data, a novel species of the genus , sp. nov., is proposed. The type strain is A35 (=IAM 15291=CCTCC AB206026).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64311-0
2007-01-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/57/1/70.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64311-0&mimeType=html&fmt=ahah

References

  1. Ash C., Priest F. G., Collins M. D. 1993; Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks, and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus . Antonie van Leeuwenhoek 64:253–260
    [Google Scholar]
  2. Chung Y. R., Kim C. H., Hwang I., Chun J. 2000; Paenibacillus koreensis sp. nov., a new species that produces an iturin-like antifungal compound. Int J Syst Evol Microbiol 50:1495–1500 [CrossRef]
    [Google Scholar]
  3. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  4. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120 [CrossRef]
    [Google Scholar]
  5. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  6. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  7. Shida O., Takagi H., Kadowaki K., Nakamura L., Komagata K. 1997; Transfer of Bacillus alginolyticus , Bacillus chondroitinus , Bacillus curdlanolyticus , Bacillus glucanolyticus , Bacillus kobensis , and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus . Int J Syst Bacteriol 47:289–298 [CrossRef]
    [Google Scholar]
  8. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  9. Swofford D. L. 1998 paup*: Phylogenetic analysis using parsimony (* and other methods), version 4 Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  10. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  11. Xie C.-H., Yokota A. 2003; Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49:345–349 [CrossRef]
    [Google Scholar]
  12. Xie C.-H., Yokota A. 2006; Reclassification of [ Flavobacterium ] ferrugineum as Terrimonas ferruginea gen. nov., comb. nov., and description of Terrimonas lutea sp. nov., isolated from soil. Int J Syst Evol Microbiol 56:1117–1121 [CrossRef]
    [Google Scholar]
  13. Yoon J.-H., Seo W.-T., Shin Y.-K., Kho Y.-H., Kang K.-H., Park Y.-H. 2002; Paenibacillus chinjuensis sp. nov., a novel exopolysaccharide-producing bacterium. Int J Syst Evol Microbiol 52:415–421
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.64311-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64311-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error