1887

Abstract

Two Gram-positive, round-spore-forming, rod-shaped, halophilic bacterial strains, 5B73C and 5B133E, were isolated from field soil in Kakegawa, Shizuoka, Japan, and were characterized taxonomically using a polyphasic approach. These two strains were found to comprise strictly aerobic, motile rods that formed subterminal endospores. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains 5B73C and 5B133E are phylogenetically affiliated to the genus , exhibiting sequence similarities of 94.1–96.4 % with respect to the type strains of species. The DNA G+C contents of strains 5B73C and 5B133E were 42.6 and 42.3 mol%, respectively. The cell-wall peptidoglycan type (-diaminopimelic acid), the major cellular fatty acids (anteiso-C, iso-C, anteiso-C and iso-C) and the quinone type (MK-7) of the isolates support their affiliation to the genus . On the basis of their genotypic and phenotypic characteristics, the isolates represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 5B73C (=IAM 15308=KCTC 13935).

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2007-07-01
2020-01-21
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References

  1. Collins, M. D. & Jones, D. ( 1981; ). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications. Microbiol Rev 45, 316–354.
    [Google Scholar]
  2. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  3. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  4. Goto, K., Omura, T., Hara, Y. & Sadaie, Y. ( 2000; ). Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus. J Gen Appl Microbiol 46, 1–8.[CrossRef]
    [Google Scholar]
  5. Goto, K., Mochida, K. M., Asahara, M., Suzuki, M. & Yokota, A. ( 2002; ). Application of the hypervariable region of the 16S rDNA sequence as an index for the rapid identification of species in the genus Alicyclobacillus. J Gen Appl Microbiol 48, 243–250.[CrossRef]
    [Google Scholar]
  6. Heyndrickx, M., Lebbe, L., Kersters, K., De Vos, P., Forsyth, C. & Logan, N. A. ( 1998; ). Virgibacillus: a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus. Int J Syst Bacteriol 48, 99–106.[CrossRef]
    [Google Scholar]
  7. Heyndrickx, M., Lebbe, L., Kersters, K., Hoste, B., De Wachter, R., De Vos, P., Forsyth, G. & Logan, N. A. ( 1999; ). Proposal of Virgibacillus proomii sp. nov. and emended description of Virgibacillus pantothenticus (Proom and Knight 1950) Heyndrickx et al. 1998 . Int J Syst Bacteriol 49, 1083–1090.[CrossRef]
    [Google Scholar]
  8. Heyrman, J., Logan, N. A., Busse, H. J., Balcaen, A., Lebbe, L., Rodriguez-Diaz, M., Swings, J. & De Vos, P. ( 2003; ). Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus Salibacillus to Virgibacillus, as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus. Int J Syst Evol Microbiol 53, 501–511.[CrossRef]
    [Google Scholar]
  9. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  10. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  11. Lee, J. S., Lim, J. M., Lee, K. C., Lee, J. C., Park, Y. H. & Kim, C. J. ( 2006; ). Virgibacillus koreensis sp. nov., a novel bacterium from a salt field, and transfer of Virgibacillus picturae comb. nov. with emended descriptions. Int J Syst Evol Microbiol 56, 251–257.[CrossRef]
    [Google Scholar]
  12. Ludwig, W., Strunk, O., Klugbauer, S., Klugbauer, N., Weizenegger, M., Neumaier, J., Bachleitner, M. & Schleifer, K.-H. ( 1998; ). Bacterial phylogeny based on comparative sequence analysis. Electrophoresis 19, 554–568.[CrossRef]
    [Google Scholar]
  13. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  14. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  15. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  16. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  17. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  18. Stackebrandt, E., Frederiksen, W., Garrity, G. M., Grimont, P. A. D., Kämpfer, P., Maiden, M. C. J., Nesme, X., Rossello-Mora, R., Swings, J. & other authors ( 2002; ). Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52, 1043–1047.[CrossRef]
    [Google Scholar]
  19. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  20. Yoon, J. H., Oh, T. K. & Park, Y. H. ( 2004; ). Transfer of Bacillus halodenitrificans Denariaz et al. 1989 to the genus Virgibacillus as Virgibacillus halodenitrificans comb. nov. Int J Syst Evol Microbiol 54, 2163–2167.[CrossRef]
    [Google Scholar]
  21. Yoon, J. H., Kang, S. J., Lee, S. Y., Lee, M. H. & Oh, T. K. ( 2005; ). Virgibacillus dokdonensis sp. nov., isolated from a Korean island, Dokdo, located at the edge of the East Sea in Korea. Int J Syst Evol Microbiol 55, 1833–1837.[CrossRef]
    [Google Scholar]
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