Two extremely halophilic archaea, strains 9-3T and 31-hongT, were isolated from Aibi salt lake and Aiding salt lake in Xin-Jiang, China. Their morphology, physiology, biochemical features, polar lipid compositions and 16S rRNA gene sequences were characterized in order to elucidate their taxonomic status. The results from this study indicated that strains 9-3T and 31-hongT are members of the genus Halorubrum. Their physiological properties and polar lipid compositions are clearly different from those of the currently described species of Halorubrum. DNA–DNA relatedness values for strain 9-3T with respect to its closely related neighbours Halorubrum saccharovorum JCM 8865T and Halorubrum lacusprofundi JCM 8891T were 51.6 and 25.1 %, respectively, DNA–DNA relatedness values for strain 31-hongT with respect to its closely related neighbours Hrr. saccharovorum JCM 8865T and Hrr. lacusprofundi JCM 8891T were 29.4 and 44.9 %, respectively, and DNA–DNA relatedness between strains 9-3T and 31-hongT was 54 %. Thus, two novel species of the genus Halorubrum are proposed, Halorubrum lipolyticum sp. nov. (type strain 9-3T=CGMCC 1.5332T=JCM 13559T) and Halorubrum aidingense sp. nov. (type strain 31-hongT=CGMCC 1.2670T=JCM 13560T).
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