1887

Abstract

Two thermophilic strains, designated 607 and 606b, were isolated from a compost pile in Japan. The novel strains were Gram-positive, aerobic, spore-forming rods. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strains 607 and 606b were closely related to (94.0–94.1 % similarity) and separated from clusters of the related genera (<91.9 %) and (91.0–92.5 %). In addition, some chemotaxonomic and physiological characteristics of strains 607 and 606b differed from those of and the two related genera. Several differences in physiological characteristics and 16S–23S rRNA gene internal transcribed spacer region nucleotide sequences were observed between strains 607 and 606b; however, DNA–DNA hybridization indicated that these two strains belonged to the same species. From these results, it is proposed that strains 607 and 606b represent the type species of a new genus, gen. nov., sp. nov., with strain 607 (=JCM 13397=DSM 17572) as the type strain. In addition, the results of phylogenetic analyses, as well as chemotaxonomic and physiological characterization, indicated that and did not belong to the genus . Based on these results, it is proposed that and should be transferred to gen. nov., comb. nov. and comb. nov., respectively.

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2006-11-01
2019-10-18
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Cellular fatty acid content of strains 607 , 606b and . [PDF](29 KB)

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Neighbour-joining phylogenetic tree and phase-contrast microscope images of spore-forming cells of strains 607 and 606b. [PDF](191 KB)

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