1887

Abstract

Using molecular and genetic analyses, 28 strains were analysed, isolated from widely different geographical localities in Europe, North America, Far-East Asia and Hawaii. Most of the strains have been assigned to the species . PCR-RAPD revealed two Japanese strains (UCD 67-278 and IFO 10138) to have peculiar patterns. Comparative rDNA (D1/D2 26S, ITS1 and ITS2) sequence analysis showed that the two strains respectively represent a novel species and a novel variety. Based on the results of sequence analysis, genetic hybridization and DNA–DNA reassociation, two new members of the genus are formally described, sp. nov. (type strain UCD 67-278=CBS 9167) and var. var. nov. (type strain IFO 10138=CBS 9168). These results show that has a worldwide distribution, while the species is represented only by the type culture CBS 2555, isolated in Indonesia. Cluster analysis revealed a correlation between PCR-RAPD fingerprints and geographical origin of the strains. Despite this molecular differentiation, strains collected in different regions of the world formed predominantly fertile hybrids, with normal recombination of control markers.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64301-0
2006-08-01
2020-01-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/8/1997.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64301-0&mimeType=html&fmt=ahah

References

  1. Bab'eva, I. P., Vustin, M. M., Naumov, G. I. & Vinovarova, M. E. ( 1986; ). Arthroascus schoenii nov. comb., a yeast-like organism. Microbiology (English translation of Mikrobiologiia) 54, 575–580.
    [Google Scholar]
  2. Bowles, J. M. & Lachance, M.-A. ( 1983; ). Patterns of variation in the yeast florae of exudates in an oak community. Can J Bot 61, 2984–2995.[CrossRef]
    [Google Scholar]
  3. Dawes, I. W. & Hardie, I. D. ( 1974; ). Selective killing of vegetative cells in sporulated yeast cultures by exposure to diethyl ether. Mol Gen Genet 131, 281–289.[CrossRef]
    [Google Scholar]
  4. Gerrits van den Ende, A. H. G. & de Hoog, G. S. ( 1999; ). Variability and molecular diagnostics of the neurotropic species Cladophialophora bantiana. Stud Mycol 43, 151–162.
    [Google Scholar]
  5. Klöcker, A. ( 1909; ). Endomyces javanensis nov. sp. C R Trav Lab Carlsberg 7, 267–272.
    [Google Scholar]
  6. Kreger-van Rij, N. J. W. ( 1970; ). Endomycopsis. In The Yeasts, a Taxonomic Study, 2nd edn, pp. 166–208. Edited by J. Lodder. Amsterdam: North-Holland.
  7. Kreger-van Rij, N. J. W. ( 1984a; ). Genus 2. Arthroascus von Arx. In The Yeasts, a Taxonomic Study, 3rd edn, pp. 114–116. Edited by N. J. W. Kreger-van Rij. Amsterdam: Elsevier.
  8. Kreger-van Rij, N. J. W. ( 1984b; ). Genus 24. Saccharomycopsis Schionning. In The Yeasts, a Taxonomic Study, 3rd edn, pp. 399–413. Edited by N. J. W. Kreger-van Rij. Amsterdam: Elsevier.
  9. Kreger-van Rij, N. J. W. & Veenhuis, M. ( 1973; ). Electron microscopy of some special cell contacts in yeasts. J Bacteriol 113, 350–356.
    [Google Scholar]
  10. Kurtzman, C. P. ( 1999; ). Two new members of the Saccharomycopsis clade: Saccharomycopsis microspora, comb. nov. and Candida lassenensis, sp. nov. Mycotaxon 71, 241–250.
    [Google Scholar]
  11. Kurtzman, C. P. & Robnett, C. J. ( 1995; ). Molecular relationships among hyphal ascomycetous yeasts and yeastlike taxa. Can J Bot 73, S824–S830.[CrossRef]
    [Google Scholar]
  12. Kurtzman, C. P. & Robnett, C. J. ( 1997; ). Identification of clinically important ascomycetous yeasts based on nucleotide divergence of the 5′ end of the large subunit (26S) ribosomal DNA gene. J Clin Microbiol 35, 1216–1223.
    [Google Scholar]
  13. Kurtzman, C. P. & Robnett, C. J. ( 1998; ). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73, 331–371.[CrossRef]
    [Google Scholar]
  14. Kurtzman, C. P. & Smith, M. Th. ( 1998; ). Saccharomycopsis Schiönning. In The Yeasts, a Taxonomic Study, 4th edn, pp. 374–386. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
  15. Lachance, M.-A. ( 1995; ). Yeast communities in a natural tequila fermentation. Antonie van Leeuwenhoek 68, 151–160.[CrossRef]
    [Google Scholar]
  16. Lachance, M.-A. & Bowles, J. M. ( 2002; ). Metschnikowia arizonensis and Metschnikowia dekortorum, two new large-spored yeast species associated with floricolous beetles. FEMS Yeast Res 2, 81–86.
    [Google Scholar]
  17. Lachance, M.-A. & Bowles, J. M. ( 2004; ). Metschinikowia similis sp. nov. and Metschnikowia colocasiae sp. nov., two ascomycetous yeasts isolated from Conotelus spp. (Coleoptera: Nitidulidae) in Costa Rica. Stud Mycol 50, 69–76.
    [Google Scholar]
  18. Lachance, M.-A., Pupovac-Velikonja, A., Natarajan, S. & Schlag-Edler, B. ( 2000; ). Nutrition and phylogeny of predacious yeasts. Can J Microbiol 46, 495–505.[CrossRef]
    [Google Scholar]
  19. Lee, C.-F., Lee, F.-L., Hsu, W.-H. & Phaff, H. J. ( 1994; ). Arthroascus fermentans, a new yeast species isolated from soil in Taiwan. Int J Syst Bacteriol 44, 303–307.[CrossRef]
    [Google Scholar]
  20. Liti, G., Peruffo, A., James, S. A., Roberts, I. N. & Louis, E. J. ( 2005; ). Inferences of evolutionary relationships from a population survey of LTR-retrotransposons and telomeric-associated sequences in the Saccharomyces sensu stricto complex. Yeast 22, 177–192.[CrossRef]
    [Google Scholar]
  21. Lodder, J. & Kreger-van Rij, N. J. W. (editors) ( 1952; ). The Yeasts a Taxonomic Study. Amsterdam: North-Holland.
  22. Mikata, K. ( 1985; ). Descriptive catalogue of IFO yeast collection. V. 30. Arthroascus javanensis (Klöcker) von Arx. IFO Res Commun 12, 112–113.
    [Google Scholar]
  23. Nadson, G. A. & Krassilnikov, N. A. ( 1932; ). Recherches sur la structure, le dévelopement et la formation des races stables chez les Endomycétacées. C R Acad Sci URSS 13, 313–332 (in Russian with French summary).
    [Google Scholar]
  24. Nakase, T. ( 1971; ). Four new yeasts found in Japan. J Gen Appl Microbiol 17, 469–478.[CrossRef]
    [Google Scholar]
  25. Naumov, G. I. ( 1987; ). Results of the genosystematics of the yeast Williopsis Zender and Zygowilliopsis Kudriavzev. Mol Genet Mikrobiol Virusol 2, 1–7 (in Russian).
    [Google Scholar]
  26. Naumov, G. I. ( 1996; ). Genetic identification of biological species in the Saccharomyces sensu stricto complex. J Ind Microbiol 17, 295–302.[CrossRef]
    [Google Scholar]
  27. Naumov, G. I. & Naumova, E. S. ( 2002; ). Five new combinations in the yeast genus Zygofabospora Kudriavzev emend. G. Naumov (pro parte Kluyveromyces) based on genetic data. FEMS Yeast Res 2, 39–46.
    [Google Scholar]
  28. Naumov, G. I., Vustin, M. M. & Babjeva, I. P. ( 1985; ). Genetic identification of two species of yeast-like fungi in the genus Arthroascus von Arx. Dokl Biol Sci (English translation of Dokl Akad Nauk) 281, 211–213.
    [Google Scholar]
  29. Naumov, G., Naumova, E., Azbukina, Z. M., Korhola, M. & Gaillardin, C. ( 1993; ). Genetic and karyotypic identification of Saccharomyces yeasts from Far East Asia. Cryptogam Mycol 14, 85–93.
    [Google Scholar]
  30. Naumov, G. I., Naumova, E. S., Kondratieva, V. I., Bulat, S. A., Mironenko, N. V., Mendonça-Hagler, L. C. & Hagler, A. N. ( 1997a; ). Genetic and molecular delineation of three sibling species in the Hansenula polymorpha complex. Syst Appl Microbiol 20, 50–56.[CrossRef]
    [Google Scholar]
  31. Naumov, G. I., Naumova, E. S. & Sniegowski, P. D. ( 1997b; ). Differentiation of European and Far East Asian populations of Saccharomyces paradoxus by allozyme analysis. Int J Syst Bacteriol 47, 341–344.[CrossRef]
    [Google Scholar]
  32. Naumov, G. I., Naumova, E. S. & Sniegowski, P. D. ( 1998; ). Saccharomyces paradoxus and Saccharomyces cerevisiae are associated with exudates of North American oaks. Can J Microbiol 44, 1045–1050.[CrossRef]
    [Google Scholar]
  33. Naumov, G. I., James, S. A., Naumova, E. S., Louis, E. J. & Roberts, I. N. ( 2000; ). Three new species in the Saccharomyces sensu stricto complex: Saccharomyces cariocanus, Saccharomyces kudriavzevii and Saccharomyces mikatae. Int J Syst Evol Microbiol 50, 1931–1942.
    [Google Scholar]
  34. Naumov, G. I., Naumova, E. S., Smith, M. T. & de Hoog, G. S. ( 2003; ). Ribosomal DNA sequencing and reinstatement of the genus Arthroascus von Arx. J Gen Appl Microbiol 49, 267–270.[CrossRef]
    [Google Scholar]
  35. Naumova, E. S., Sukhotina, N. N. & Naumov, G. I. ( 2004; ). Molecular-genetic differentiation of the dairy yeast Kluyveromyces lactis and its closest wild relatives. FEMS Yeast Res 5, 263–269.[CrossRef]
    [Google Scholar]
  36. Phaff, H. J. & Knapp, E. P. ( 1956; ). The taxonomy of yeasts found in exudates of certain trees and other natural breeding sites of some species of Drosophila. Antonie van Leeuwenhoek 22, 117–130.[CrossRef]
    [Google Scholar]
  37. Phaff, H. J., Miller, M. W., Yoneyama, M. & Soneda, M. ( 1972; ). A comparative study of the yeast florae associated with trees on the Japanese islands and on the west coast of North America. In Fermentation Technology Today, pp. 759–774. Edited by G. Terui. Osaka: Society of Fermentation Technology.
  38. Pimenta, R. S., Silva, F. L., Corrêa, A. & Rosa, C. A. ( 2004; ). Biological control of Penicillium italicum and P. expansum by the predacious yeast Saccharomycopsis javanensis on oranges. In Congress Book. Eleventh International Congress on Yeasts (ICY2004), p. 77. Rio de Janeiro, Brazil, 15–20 August 2004.
  39. Pitt, J. I. & Miller, M. W. ( 1970; ). Speciation in the yeast genus Metschnikowia. Antonie van Leeuwenhoek 36, 357–381.[CrossRef]
    [Google Scholar]
  40. Smith, M. Th., Poot, G. A. & Batenburg-van der Vegte, W. H. ( 1990; ). Genome comparison among species of the genus Arthroascus von Arx. Antonie van Leeuwenhoek 58, 249–253.[CrossRef]
    [Google Scholar]
  41. Smith, M. Th., de Cock, A. W. M., Poot, G. A. & Steensma, H. Y. ( 1995; ). Genome comparison in the yeastlike fungal genus Galactomyces Redhead et Malloch. Int J Syst Bacteriol 45, 826–831.[CrossRef]
    [Google Scholar]
  42. Stelling-Dekker, N. M. ( 1931; ). Die Sporogen Hefen Teil 1. Verh K Ned Akad Wetenschap Afd Natuurk Sect II 28, 1–547 (in Dutch).
    [Google Scholar]
  43. Van de Peer, Y. & De Wachter, R. ( 1994; ). treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10, 569–570.
    [Google Scholar]
  44. von Arx, J. A. ( 1972; ). On Endomyces, Endomycopsis and related yeast-like fungi. Antonie van Leeuwenhoek 38, 289–309.[CrossRef]
    [Google Scholar]
  45. Yamada, Y., Higashi, T. & Mikata, K. ( 1998; ). The phylogeny of species of the ascogenous teleomorphic yeast genera Ambrosiozyma, Hormoascus, Hyphopichia, Arthroascus, and Botryoascus based on the partial sequences of 18S and 26S ribosomal RNAs. Bull Fac Agric Shizuoka Univ 48, 1–13.
    [Google Scholar]
  46. Yarrow, D. ( 1998; ). Methods for the isolation, maintenance and identification of yeasts. In The Yeasts, a Taxonomic Study, 4th edn, pp. 77–100. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64301-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64301-0
Loading

Data & Media loading...

Supplements

[PDF file of Supplementary Tables S1 and S2](24 KB)

PDF

Relationships between strains based on mean DNA–DNA reassociation values expressed as percentages. [PDF](14 KB)

PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error