Among a large collection of Taiwanese soil isolates, a novel Gram-negative, rod-shaped, non-spore-forming, yellow-pigmented bacterial strain, Soil-3-27T, was isolated from farmland soil in Wu-Feng, Taiwan. The isolate was subjected to a polyphasic study including 16S rRNA gene sequencing, DNA–DNA hybridization, fatty acid analysis and comparative phenotypic characterization. The 16S rRNA gene sequence analysis indicated that the organism belongs to the genus Chryseobacterium. The organism contains menaquinone MK-6 as the predominant isoprenoid quinone and 15 : 0 iso (43 %), 17 : 1 isoω9c (17.5 %) and 17 : 0 iso 3-OH (16.6 %) as the major fatty acids. Phylogenetically, the closest relatives of strain Soil-3-27T are Chryseobacterium daecheongense, Chryseobacterium defluvii and Chryseobacterium taichungense with 96.7–97.2 % sequence similarity. DNA–DNA hybridization showed relatedness values of 8.5–24.2 % with these species. The DNA G+C content is 36.8 mol%. Strain Soil-3-27T is clearly distinguishable from other Chryseobacterium species and represents a novel species, for which the name Chryseobacterium taiwanense sp. nov. is proposed. The type strain is strain Soil-3-27T (=BCRC 17412T=IAM 15317T=LMG 23355T).
ChernL.-L.,
StackebrandtE.,
LeeS.-F.,
LeeF.-L.,
ChenJ.-K.,
FuH.-M.2004; Chitinibacter tainanensis gen. nov., sp. nov. a chitin-degrading aerobe from soil in Taiwan. Int J Syst Evol Microbiol 54:1387–1391[CrossRef]
CollinsM. D.,
JonesD.1981; A note on the separation of natural mixtures of bacterial ubiquinones using reverse-phase partition thin-layer chromatography and high performance liquid chromatography. J Appl Bacteriol 51:129–134[CrossRef]
de BeerH.,
HugoC. J.,
JoosteP. J.,
WillemsA.,
VancanneytM.,
CoenyeT.,
VandammeP. A. R.2005; Chryseobacterium vrystaatense sp. nov., isolated from raw chicken in a chicken-processing plant. Int J Syst Evol Microbiol 55:2149–2153[CrossRef]
EzakiT.,
HashimotoY.,
YabuuchiE.1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229[CrossRef]
GerhardtP.,
MurrayR. G. E.,
CostilowR. N.,
NesterE. W.,
WoodW. A.,
KriegN. R.,
PhillipsG. B.1981Manual of Methods for General Bacteriology Washington, DC: American Society for Microbiology;
HolmesB.,
OwenR. J.,
SteigerwaltA. G.,
BrennerD. J.1984; Flavobacterium gleum , a new species found in human clinical specimens. Int J Syst Bacteriol 34:21–25[CrossRef]
KimK. K.,
KimM. K.,
LimJ. H.,
ParkH. Y.,
LeeS. T.2005; Transfer of Chryseobacterium meningosepticum and Chryseobacterium miricola to Elizabethkingia gen. nov. as Elizabethkingia meningoseptica comb. nov. and Elizabethkingia miricola comb. nov. Int J Syst Evol Microbiol 55:1287–1293[CrossRef]
LapageS. P.,
SneathP. H. A.,
LesselE. F.,
SkermanV. B. D.,
SeeligerH. P. R.,
ClarkW. A.1992International Code of Nomenclature of Bacteria (1990 revision) Washington, DC: American Society for Microbiology;
LiY.,
KawamuraY.,
FujiwaraN.,
NakaT.,
LiuH.,
HuangX.,
KobayashiK.,
EzakiT.2003; Chryseobacterium miricola sp. nov., a novel species isolated from condensation water of space station Mir. Syst Appl Microbiol 26:523–528[CrossRef]
TamaokaJ.,
KomagataK.1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128[CrossRef]
ThompsonJ. D.,
GibsonT. J.,
PlewniakF.,
JeanmouginF.,
HigginsD. G.1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882[CrossRef]
VandammeP.,
BernardetJ.-F.,
SegersP.,
KerstersK.,
HolmesB.1994; New perspectives in the classification of the flavobacteria: description of Chryseobacterium gen.nov., Bergeyella gen. nov., and Empedobacter nom. rev. Int J Syst Bacteriol 44:827–831[CrossRef]
YamaguchiS.,
YokoeM.2000; A novel protein-deamidating enzyme from Chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil. Appl Environ Microbiol 66:3337–3343[CrossRef]