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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 56, Issue 12

New insight into diversity in the genus , including the description of ten novel species Free

Abstract

We investigated the diversity of a collection of 76 strains, isolated from at least 27 species of nematodes and collected in 32 countries, using three complementary approaches: 16S rRNA gene sequencing, molecular typing and phenotypic characterization. The 16S rRNA gene sequences of the strains were highly conserved (similarity coefficient >95 %), suggesting that the common ancestor of the genus probably emerged between 250 and 500 million years ago. Based on comparisons of the 16S rRNA gene sequences, we identified 13 groups and seven unique sequences. This classification was confirmed by analysis of molecular typing profiles of the strains, leading to the classification of new isolates into the species described previously and the description of ten novel species: sp. nov. (type strain USTX62=CIP 109066=DSM 17905), sp. nov. (type strain FRM16=CIP 109074=DSM 17909), sp. nov. (type strain ID10=CIP 109073=DSM 17911), sp. nov. (type strain KE01=CIP 109072=DSM 17903), sp. nov. (type strain USNJ01=CIP 109199=DSM 18168), sp. nov. (type strain SaV=CIP 109068=DSM 17907), sp. nov. (type strain VC01=CIP 109075=DSM 17908), sp. nov. (type strain Q1=CIP 109069=DSM 17902), sp. nov. (type strain PR06-A=CIP 109070=DSM 17910) and sp. nov. (type strain TH01=CIP 109067=DSM 17904). The strains studied here had very similar phenotypic patterns, but phenotypic features nonetheless differentiated the following species: , , , , , and . Based on phenotypic analysis, we identified two major groups of strains. Phenotypic group G comprised strains able to grow at temperatures of 35–42 °C, whereas phenotypic group G comprised strains that grew at temperatures below 35 °C, suggesting that some species may be adapted to tropical or temperate regions and/or influenced by the growth and development temperature of their nematode host.

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2006-12-01
2024-04-20
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New insight into diversity in the genus Xenorhabdus, including the description of ten novel species
Int J Syst Evol Microbiol 56, 2805 (2006); https://doi.org/10.1099/ijs.0.64287-0
/content/journal/ijsem/10.1099/ijs.0.64287-0
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