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Abstract

Strain XH-48 was isolated from the sediment of Lake Xilinhot, a saline lake in Inner Mongolia (China). The organism is pleomorphic, neutrophilic and requires at least 2.5 M (15 %) NaCl, but not MgCl, for growth; it exhibits optimal growth at 3.4 M (20 %) NaCl. The G+C content of its DNA is 61 mol%. 16S rRNA gene sequence analysis revealed that strain XH-48 is a member of the family , but there were low levels of similarity with other members of this family. The highest sequence similarity values (94.5 and 93.3 %) were obtained with the 16S rRNA genes of and , respectively. Polar lipid analyses revealed that strain XH-48 contains phosphatidylglycerol and phosphatidylglyceromethylphosphate, derived from both CC and CC glycerol diethers, and two unidentified glycolipids. On the basis of the data obtained, the novel isolate cannot be classified within any recognized genus. Strain XH-48 should be placed within a novel genus and species within the family , order , for which the name gen. nov., sp. nov. is proposed. The type strain of is strain XH-48 (=DSM 17691=CGMCC 1.5338=JCM 13463=CECT 7116).

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2006-07-01
2019-10-18
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References

  1. Arahal, D. R., Dewhirst, F. E., Paster, B. J., Volcani, B. E. & Ventosa, A. ( 1996; ). Phylogenetic analyses of some extremely halophilic archaea isolated from Dead Sea water, determined on the basis of their 16S rRNA sequences. Appl Environ Microbiol 62, 3779–3786.
    [Google Scholar]
  2. Castillo, A. M., Gutiérrez, M. C., Kamekura, M., Ma, Y., Cowan, D. A., Jones, B. E., Grant, W. D. & Ventosa, A. ( 2006; ). Halovivax asiaticus gen. nov., sp. nov., a novel extremely halophilic archaeon isolated from Inner Mongolia, China. Int J Syst Evol Microbiol 56, 765–770.[CrossRef]
    [Google Scholar]
  3. Fan, H., Xue, Y., Ma, Y., Ventosa, A. & Grant, W. D. ( 2004; ). Halorubrum tibetense sp. nov., a novel haloalkaliphilic archaeon from Lake Zabuye in Tibet, China. Int J Syst Evol Microbiol 54, 1213–1216.[CrossRef]
    [Google Scholar]
  4. Feng, J., Zhou, P. & Liu, S. ( 2004; ). Halorubrum xinjiangense sp. nov., a novel halophile isolated from saline lakes in China. Int J Syst Evol Microbiol 54, 1789–1791.[CrossRef]
    [Google Scholar]
  5. Feng, J., Zhou, P., Zhou, Y., Liu, S. & Warren-Rhodes, K. ( 2005; ). Halorubrum alkaliphilum sp. nov., a novel haloalkaliphile isolated from a soda lake in Xinjiang, China. Int J Syst Evol Microbiol 55, 149–152.[CrossRef]
    [Google Scholar]
  6. Gibbons, N. E. ( 1974; ). Family V. Halobacteriaceae fam. nov. In Bergey's Manual of Determinative Bacteriology, 8th edn, pp. 269–270. Edited by R. E. Buchanan & N. E. Gibbons. Baltimore: Williams & Wilkins.
  7. Gonzalez, C., Gutierrez, C. & Ramírez, C. ( 1978; ). Halobacterium vallismortis sp. nov. An amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 24, 710–715.[CrossRef]
    [Google Scholar]
  8. Grant, W. D. ( 2001a; ). Genus I. Halobacterium Elazari-Volcani 1957, 207,AL emend. Larsen and Grant 1989, 2222. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 301–305. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  9. Grant, W. D. ( 2001b; ). Genus IV. Halococcus Schoop 1935a, 817AL. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 311–314. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  10. Grant, W. D., Kamekura, M., McGenity, T. J. & Ventosa, A. ( 2001; ). Order I. Halobacteriales Grant and Larsen 1989b, 495VP (Effective publication: Grant and Larsen 1989a, 2216). In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 294–299. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  11. Hezayen, F. F., Tindall, B. J., Steinbüchel, A. & Rehm, B. H. A. ( 2002; ). Characterization of a novel halophilic archaeon, Halobiforma haloterrestris gen. nov., sp. nov., and transfer of Natronobacterium nitratireducens to Halobiforma nitratireducens comb. nov. Int J Syst Evol Microbiol 52, 2271–2280.[CrossRef]
    [Google Scholar]
  12. Itoh, T., Yamaguchi, T., Zhou, P. & Takashina, T. ( 2005; ). Natronolimnobius baerhuensis gen. nov., sp. nov. and Natronolimnobius innermongolicus sp. nov., novel haloalkaliphilic archaea isolated from soda lakes in Inner Mongolia, China. Extremophiles 9, 111–116.[CrossRef]
    [Google Scholar]
  13. Kamekura, M. ( 1993; ). Lipids of extreme halophiles. In The Biology of Halophilic Bacteria, pp. 135–161. Edited by R. H. Vreeland & L. I. Hochstein. Boca Raton, FL: CRC Press.
  14. Kamekura, M. & Dyall-Smith, M. L. ( 1995; ). Taxonomy of the family Halobacteriaceae and the description of two genera Halorubrobacterium and Natrialba. J Gen Appl Microbiol 41, 333–350.[CrossRef]
    [Google Scholar]
  15. Kamekura, M., Dyall-Smith, M. L., Upasani, V., Ventosa, A. & Kates, M. ( 1997; ). Diversity of alkaliphilic halobacteria: proposals for transfer of Natronobacterium vacuolatum, Natronobacterium magadii, and Natronobacterium pharaonis to Halorubrum, Natrialba and Natronomonas gen. nov., respectively, as Halorubrum vacuolatum comb. nov., Natrialba magadii comb. nov., and Natronomonas pharaonis comb. nov., respectively. Int J Syst Bacteriol 47, 853–857.[CrossRef]
    [Google Scholar]
  16. Kamekura, M., Mizuki, T., Usami, R., Yoshida, Y., Horikoshi, K. & Vreeland, R. H. ( 2004; ). The potential use of signature bases from 16S rRNA gene sequences to aid the assignment of microbial strains to genera of halobacteria. In Halophilic Microorganisms, pp. 77–87. Edited by A. Ventosa. Heidelberg: Springer.
  17. Lopez-Garcia, P., Moreira, D., Lopez-Lopez, A. & Rodríguez-Valera, F. ( 2001; ). A novel haloarchaeal-related lineage is widely distributed in deep oceanic regions. Environ Microbiol 3, 72–78.[CrossRef]
    [Google Scholar]
  18. Ludwig, W., Strunk, O., Westram, R. & 29 other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  19. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  20. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  21. McGenity, T. J. & Grant, W. D. ( 1995; ). Transfer of Halobacterium saccharovorum, Halobacterium sodomense, Halobacterium trapanicum NRC 34021 and Halobacterium lacusprofundi to the genus Halorubrum gen. nov., as Halorubrum saccharovorum comb nov., Halorubrum sodomense comb nov., Halorubrum trapanicum comb. nov., and Halorubrum lacusprofundi comb. nov. Syst Appl Microbiol 18, 237–243.[CrossRef]
    [Google Scholar]
  22. McGenity, T. J., Gemmell, R. T. & Grant, W. D. ( 1998; ). Proposal of a new halobacterial genus Natrinema gen. nov., with two species Natrinema pellirubrum nom. nov. and Natrinema pallidum nom. nov. Int J Syst Bacteriol 48, 1187–1196.[CrossRef]
    [Google Scholar]
  23. Montalvo-Rodríguez, R., Vreeland, R. H., Oren, A., Kessel, M., Betancourt, C. & Lopez-Garriga, J. ( 1998; ). Halogeometricum borinquense gen. nov., sp. nov., a novel halophilic archaeon from Puerto Rico. Int J Syst Bacteriol 48, 1305–1312.[CrossRef]
    [Google Scholar]
  24. Oren, A. ( 2001; ). The order Halobacteriales. In The Prokaryotes. An Evolving Electronic Resource for the Microbiological Community. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer. http://141.150.157.117:8080/prokPUB/index.htm
  25. Oren, A., Gurevich, P., Gemmell, R. T. & Teske, A. ( 1995; ). Halobaculum gomorrense gen. nov., sp. nov., a novel extremely halophilic archaeon from the Dead Sea. Int J Syst Bacteriol 45, 747–754.[CrossRef]
    [Google Scholar]
  26. Oren, A., Ventosa, A. & Grant, W. D. ( 1997; ). Proposed minimal standards for description of new taxa in the order Halobacteriales. Int J Syst Bacteriol 47, 233–238.[CrossRef]
    [Google Scholar]
  27. Oren, A., Elevi, R., Watanabe, S., Ihara, K. & Corcelli, A. ( 2002; ). Halomicrobium mukohataei gen. nov., comb. nov., and emended description of Halomicrobium mukohataei. Int J Syst Evol Microbiol 52, 1831–1835.[CrossRef]
    [Google Scholar]
  28. Owen, R. J. & Hill, L. R. ( 1979; ). The estimation of base compositions, base pairing and genome size of bacterial deoxyribonucleic acids. In I dentification Methods for Microbiologists, 2nd edn, pp. 217–296. Edited by F. A. Skinner & D. W. Lovelock. London: Academic Press.
  29. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  30. Tindall, B. J. ( 1992; ). The family Halobacteriaceae. In The Prokaryotes. A Handbook of Bacteria: Ecophysiology, Isolation, Identification, Applications, 2nd edn, vol. 1, pp. 768–808. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  31. Tindall, B. J., Ross, H. N. M. & Grant, W. D. ( 1984; ). Natronobacterium gen. nov. and Natronococcus gen. nov., two new genera of haloalkaliphilic archaebacteria. Syst Appl Microbiol 5, 41–57.[CrossRef]
    [Google Scholar]
  32. Torreblanca, M., Rodriguez-Valera, F., Juez, G., Ventosa, A., Kamekura, M. & Kates, M. ( 1986; ). Classification of non-alkaliphilic halobacteria based on numerical taxonomy and polar lipid composition and description of Haloarcula gen. nov. and Haloferax gen. nov. Syst Appl Microbiol 8, 89–99.[CrossRef]
    [Google Scholar]
  33. Ventosa, A., Gutiérrez, M. C., Kamekura, M. & Dyall-Smith, M. L. ( 1999; ). Proposal to transfer Halococcus turkmenicus, Halobacterium trapanicum JCM 9743 and strain GSL-11 to Haloterrigena turkmenica gen. nov., comb. nov. Int J Syst Bacteriol 49, 131–136.[CrossRef]
    [Google Scholar]
  34. Vreeland, R. H., Straight, S., Krammes, J., Dougherty, K., Rosenzweig, W. D. & Kamekura, M. ( 2002; ). Halosimplex carlsbadense gen. nov., sp. nov., a unique halophilic archaeon, with three 16S rRNA genes, that grows only in defined medium with glycerol and acetate or pyruvate. Extremophiles 6, 445–452.[CrossRef]
    [Google Scholar]
  35. Wainø, M., Tindall, B. J. & Ingvorsen, K. ( 2000; ). Halorhabdus utahensis gen. nov., sp. nov., an aerobic, extremely halophilic member of the Archaea from Great Salt Lake, Utah. Int J Syst Evol Microbiol 50, 183–190.[CrossRef]
    [Google Scholar]
  36. Wilson, K. ( 1987; ). Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology, pp. 2.4.1–2.4.2. Edited by F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith & K. Struhl. New York: Wiley.
  37. Xin, H., Itoh, T., Zhou, P., Suzuki, K., Kamekura, M. & Nakase, Y. ( 2000; ). Natrinema versiforme sp. nov., an extremely halophilic archaeon from Aibi salt lake, Xinjiang, China. Int J Syst Evol Microbiol 50, 1297–1303.[CrossRef]
    [Google Scholar]
  38. Xin, H., Itoh, T., Zhou, P., Suzuki, K. & Nakase, Y. ( 2001; ). Natronobacterium nitratireducens sp. nov., a haloalkaliphilic archaeon isolated from a soda lake in China. Int J Syst Evol Microbiol 51, 1825–1829.[CrossRef]
    [Google Scholar]
  39. Xu, Y., Zhou, P. & Tian, X. ( 1999; ). Characterization of two novel haloalkaliphilic archaea, Natronorubrum bangense gen. nov., sp. nov. and Natronorubrum tibetense gen. nov., sp. nov. Int J Syst Bacteriol 49, 261–266.[CrossRef]
    [Google Scholar]
  40. Xu, Y., Wang, Z., Xue, Y., Zhou, P., Ma, Y., Ventosa, A. & Grant, W. D. ( 2001; ). Natrialba hulunbeirensis sp. nov. and Natrialba chahannaoensis sp. nov., novel haloalkaliphilic archaea from soda lakes in Inner Mongolia Autonomous Region, China. Int J Syst Evol Microbiol 51, 1693–1698.[CrossRef]
    [Google Scholar]
  41. Xu, X.-W., Ren, P.-G., Liu, S.-J., Wu, M. & Zhou, P.-J. ( 2005a; ). Natrinema altunense sp. nov., an extremely halophilic archaeon isolated from a salt lake in Altun Mountain of Xin-Jiang, China. Int J Syst Evol Microbiol 55, 1311–1314.[CrossRef]
    [Google Scholar]
  42. Xu, X.-W., Wu, M., Zhou, P.-J. & Liu, S.-J. ( 2005b; ). Halobiforma lacisalsi sp. nov., isolated from a lake in China. Int J Syst Evol Microbiol 55, 1949–1952.[CrossRef]
    [Google Scholar]
  43. Xu, X.-W., Liu, S.-J., Tohty, D., Oren, A., Wu, M. & Zhou, P.-J. ( 2005c; ). Haloterrigena saccharevitans sp. nov., an extremely halophilic archaeon from Xin-Jiang, China. Int J Syst Evol Microbiol 55, 2539–2542.[CrossRef]
    [Google Scholar]
  44. Xue, Y., Fan, H., Ventosa, A., Grant, W. D., Jones, B. E., Cowan, D. A. & Ma, Y. ( 2005; ). Halalkalicoccus tibetensis gen. nov., sp. nov., representing a novel genus of haloalkaliphilic archaea. Int J Syst Evol Microbiol 55, 2501–2505.[CrossRef]
    [Google Scholar]
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vol. , part 7, pp. 1519 - 1524

Thin-layer chromatography of polar lipids extracted from strain XH-48 and some other haloarchaea. Lanes: 1, NRC-1; 2, EJ-46 ; 3, sp. A8; 4, sp. A6; 5, sp. A7; 6, strain XH-48 ; 7, NRC-1. Circled spots are glycolipids.



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