1887

Abstract

A novel aerobic, Gram-negative bacterium, named 13IX/A01/164, was isolated from surface waters in the coastal north-western Mediterranean Sea. Cells were motile, straight rods, 2.5 μm long and 0.2 μm wide, and formed orange colonies on marine agar medium. The G+C content of the genomic DNA of strain 13IX/A01/164 was 42 mol%. Phylogenetic analysis of the 16S rRNA gene sequence placed the strain in the phylum within the family . On the basis of 16S rRNA gene sequence comparison and physiological and biochemical characteristics, this isolate represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is 13IX/A01/164 (=DSM 17893=CIP 109092=OOB 256).

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2006-08-01
2019-10-20
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References

  1. Agogué, H., Casamayor, E. O., Joux, F. & 8 other authors ( 2004; ). Comparison of samplers for the biological characterization of the sea surface microlayer. Limnol Oceanogr Methods 2, 213–225.[CrossRef]
    [Google Scholar]
  2. Agogué, H., Casamayor, E. O., Bourrain, M., Obernosterer, I., Joux, F., Herndl, G. J. & Lebaron, P. ( 2005; ). A survey on bacteria inhabiting the sea surface microlayer of coastal ecosystems. FEMS Microbiol Ecol 54, 269–280.[CrossRef]
    [Google Scholar]
  3. Alfredsson, G. A., Kristjansson, J. K., Hjörleifsdottir, S. & Stetter, K. O. ( 1988; ). Rhodothermus marinus gen. nov., a thermophilic, halophilic bacterium from submarine hot springs in Iceland. J Gen Microbiol 134, 299–306.
    [Google Scholar]
  4. Altschul, S. F., Madden, T. L., Schaffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  5. Antón, J., Oren, A., Benlloch, S., Rodríguez-Valera, F., Amann, R. & Rosselló-Mora, R. ( 2002; ). Salinibacter ruber gen. nov., sp. nov., a novel, extremely halophilic member of the Bacteria from saltern crystallizer ponds. Int J Syst Evol Microbiol 52, 485–491.
    [Google Scholar]
  6. De Groote, D., van Doorn, L. J., Ducatelle, R., Verschuuren, A., Tilmant, K., Quint, W. G. V., Haesebrouck, F. & Vandamme, P. ( 1999; ). Phylogenetic characterization of ‘Candidatus Helicobacter bovis’, a new gastric helicobacter in cattle. Int J Syst Bacteriol 49, 1707–1715.[CrossRef]
    [Google Scholar]
  7. Demirjian, D. C., Moris-Varas, F. & Cassidy, C. S. ( 2001; ). Enzymes from extremophiles. Curr Opin Chem Biol 5, 144–151.[CrossRef]
    [Google Scholar]
  8. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  9. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  10. Galtier, N., Gouy, M. & Gautier, C. ( 1996; ). seaview and phylo_win: two graphic tools for sequence alignment and molecular phylogeny. Comput Appl Biosci 12, 543–548.
    [Google Scholar]
  11. Huber, R. & Stetter, K. O. ( 1992; ). The order Thermotogales. In The Prokaryotes, 2nd edn, vol. IV, pp. 3809–3815. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K.-H. Schleifer. New York: Springer.
  12. Hudson, J. A., Schofield, K. M., Morgan, H. W. & Daniel, R. M. ( 1989; ). Thermonema lapsum gen. nov., sp. nov., a thermophilic gliding bacterium. Int J Syst Bacteriol 39, 485–487.[CrossRef]
    [Google Scholar]
  13. Imhoff, J. F. & Süling, J. ( 1996; ). The phylogenetic relationship among Ectothiorhodospiraceae: a reevaluation of their taxonomy on the basis of 16S rDNA analyses. Arch Microbiol 165, 106–113.[CrossRef]
    [Google Scholar]
  14. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  15. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 4, 109–118.
    [Google Scholar]
  16. McCammon, S. A. & Bowman, J. P. ( 2000; ). Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov. and Flavobacterium xanthum sp. nov., nom. rev., and reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov. Int J Syst Evol Microbiol 50, 1055–1063.[CrossRef]
    [Google Scholar]
  17. Powers, E. ( 1995; ). Efficacy of the Ryu nonstaining KOH technique for rapidly determining Gram reactions of food-borne and waterborne bacteria and yeasts. Appl Environ Microbiol 61, 3756–3758.
    [Google Scholar]
  18. Raguénès, G., Christen, R., Guézennec, J., Pignet, P. & Barbier, G. ( 1997; ). Vibrio diabolicus sp. nov., a new polysaccharide-secreting organism isolated from a deep-sea hydrothermal vent polychaete annelid, Alvinella pompejana. Int J Syst Bacteriol 47, 989–995.[CrossRef]
    [Google Scholar]
  19. Rainey, F. A. & da Costa, M. S. ( 2001; ). Order II. Thermales ord. nov. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1 (The Archaea and the Deeply Branching and Phototrophic Bacteria), p. 403. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer-Verlag.
  20. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  21. Sako, Y., Takai, K., Ishida, Y., Uchida, A. & Katayama, Y. ( 1996; ). Rhodothermus obamensis sp. nov., a modern lineage of extremely thermophilic marine bacteria. Int J Syst Bacteriol 46, 1099–1104.[CrossRef]
    [Google Scholar]
  22. Silva, Z., Horta, C., da Costa, M. S., Chung, A. P. & Rainey, F. A. ( 2000; ). Polyphasic evidence for the reclassification of Rhodothermus obamensis Sako et al. 1996 as a member of the species Rhodothermus marinus Alfredsson et al. 1988 . Int J Syst Evol Microbiol 50, 1457–1461.[CrossRef]
    [Google Scholar]
  23. Tenreiro, S., Nobre, M. F., Rainey, F. A., Miguel, C. & da Costa, M. S. ( 1997; ). Thermonema rossianum sp. nov., a new thermophilic and slightly halophilic species from saline hot spring in Naples, Italy. Int J Syst Bacteriol 47, 122–126.[CrossRef]
    [Google Scholar]
  24. Wery, N., Lesongeur, F., Pignet, P., Derennes, V., Cambon-Bonavita, M. A., Godfroy, A. & Barbier, G. ( 2001a; ). Marinitoga camini gen. nov., sp. nov., a rod-shaped bacterium belonging to the order Thermotogales, isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 51, 495–504.
    [Google Scholar]
  25. Wery, N., Moricet, J. M., Cueff, V., Jean, J., Pignet, P., Lesongeur, F., Cambon-Bonavita, M. A. & Barbier, G. ( 2001b; ). Caloranaerobacter azorensis gen. nov., sp. nov., an anaerobic thermophilic bacterium isolated from a deep-sea hydrothermal vent. Int J Syst Evol Microbiol 51, 1789–1796.[CrossRef]
    [Google Scholar]
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vol. , part 8, pp. 1883 - 1887

Effects of temperature, NaCl concentration and pH on the growth of strain 13IX/A01/164 . [PDF](14 KB)



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