1887

Abstract

A motile, Gram-positive, boron-tolerant and moderately halotolerant rod-shaped bacterium was isolated from a soil naturally high in boron minerals found in the Hisarcik area of Turkey. The novel isolate, designated T-16X, produced spherical or ellipsoidal endospores in a non-bulging or slightly swollen sporangium in a terminal position and survived in a medium containing up to 450 mM boron. Whereas it tolerated 11 % (w/v) NaCl, it also grew without NaCl or boron. The temperature range for growth was 16–37 °C (optimum 25–28 °C) and the pH range for growth was 6.0–10.0 (optimum pH 7.5–8.5). The DNA G+C content was 35.8 mol% and the major cellular fatty acids were iso-C and anteiso-C at 18.2 and 45.7 % of the total fatty acids, respectively. MK-7 (90 %) was the predominant respiratory quinone system and -diaminopimelic acid was the predominant diamino acid of the cell-wall peptidoglycan. Phylogenetic analysis of the 16S rRNA gene sequence revealed that the novel strain is closely related to the type strains of (96.7 % similarity), (95.5 %) and (95.4 %). However, the maximum DNA hybridization value for this strain with these closely related strains was less than 26.2 %. On the basis of 16S rRNA gene sequence data and chemotaxonomic and physiological features, the organism T-16X (=DSM 17256=IAM 15263=ATCC BAA-1190) is proposed to be a member of the genus as the type strain of the novel species sp. nov.

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2007-04-01
2019-10-19
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vol. , part 4, pp. 796 - 802

Scanning electron micrographs of cells of strain T-16X grown on BUG agar medium (Biolog) containing 20 mM boron and 1 % NaCl for 6 days.

Growth curves for strain T-16X (a) and DH10B (b) grown at different levels of boron supply.

Phylogenetic trees showing the interrelationships of strain T-16X and closely related species inferred from 16S rRNA gene sequences, constructed using the parsimony method (a) and maximum-likelihood method (b).

Polar lipid profile of strain T-16X analysed using two-dimensional TLC.



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