1887

Abstract

A low-G+C-content Gram-positive bacterium, designated CV53, phylogenetically related to species of the genus , was isolated from a highly alkaline non-saline groundwater environment (pH 11.4). This organism comprised rod-shaped cells, was aerobic, did not display spore formation, was catalase- and oxidase-negative, had an optimum growth temperature of 40 °C and had an optimum pH of approximately 7.0–8.5. Optimal growth was observed in the absence of NaCl, but growth did occur at NaCl concentrations up to 3.0 %. The strain possessed an A1-type peptidoglycan cell wall and the major respiratory quinone was MK-7. The predominant fatty acids were anteiso-C, iso-C and anteiso-C. The G+C content of the DNA was 43.1 mol%. Phylogenetic analyses of the 16S rRNA gene sequence revealed that the novel isolate is closely related to the type strain of , forming a coherent cluster supported by bootstrap analysis at a confidence level of 90 %. The pairwise similarity of the 16S rRNA gene sequences of the two strains is 97.7 %. On the basis of the phylogenetic analyses and the distinct phenotypic characteristics, strain CV53 represents a novel species within the genus , for which we propose the name sp. nov. The type strain is CV53 (=LMG 23174=CIP 108889).

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2006-11-01
2020-01-29
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vol. , part 11, pp. 2571 - 2574

Fatty acid compositions of sp. nov. CV-53 and YKJ-10 . [PDF](26 KB)



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