1887

Abstract

A low-G+C-content Gram-positive bacterium, designated CV53, phylogenetically related to species of the genus , was isolated from a highly alkaline non-saline groundwater environment (pH 11.4). This organism comprised rod-shaped cells, was aerobic, did not display spore formation, was catalase- and oxidase-negative, had an optimum growth temperature of 40 °C and had an optimum pH of approximately 7.0–8.5. Optimal growth was observed in the absence of NaCl, but growth did occur at NaCl concentrations up to 3.0 %. The strain possessed an A1-type peptidoglycan cell wall and the major respiratory quinone was MK-7. The predominant fatty acids were anteiso-C, iso-C and anteiso-C. The G+C content of the DNA was 43.1 mol%. Phylogenetic analyses of the 16S rRNA gene sequence revealed that the novel isolate is closely related to the type strain of , forming a coherent cluster supported by bootstrap analysis at a confidence level of 90 %. The pairwise similarity of the 16S rRNA gene sequences of the two strains is 97.7 %. On the basis of the phylogenetic analyses and the distinct phenotypic characteristics, strain CV53 represents a novel species within the genus , for which we propose the name sp. nov. The type strain is CV53 (=LMG 23174=CIP 108889).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64281-0
2006-11-01
2021-03-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/11/2571.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64281-0&mimeType=html&fmt=ahah

References

  1. Combet-Blanc Y., Ollivier B., Streicher C., Patel B. K., Dwivedi P. P., Pot B., Prensier G., Garcia J. L. 1995; Bacillus thermoamylovorans sp. nov., a moderately thermophilic and amylolytic bacterium. Int J Syst Bacteriol 45:9–16 [CrossRef]
    [Google Scholar]
  2. Duckworth A. W., Grant S., Grant W. D., Jones B. E., Meijer D. 1998; Dietzia natronolimnaios sp. nov., a new member of the genus Dietzia isolated from an East African soda lake. Extremophiles 2:359–366 [CrossRef]
    [Google Scholar]
  3. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [CrossRef]
    [Google Scholar]
  4. Jones E. B., Grant W. D., Collins N. C., Mwatha W. E. 1994; Alkaliphiles: diversity and identification. In Bacterial Diversity and Systematics pp  195–230 Edited by Priest F. G. New York: Plenum Press;
    [Google Scholar]
  5. Jones E. B., Grant W. D., Duckworth A. W., Owenson G. G. 1998; Microbial diversity of soda lakes. Extremophiles 2:191–200 [CrossRef]
    [Google Scholar]
  6. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp  21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  7. Ludwig W., Strunk O., Westram R. 29 other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [CrossRef]
    [Google Scholar]
  8. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  9. Nielsen P., Fritze D., Priest F. G. 1995; Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. Microbiology 141:1745–1761 [CrossRef]
    [Google Scholar]
  10. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  11. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156
    [Google Scholar]
  12. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  13. Tiago I., Chung A., Veríssimo A. 2004; Bacterial diversity in a nonsaline alkaline environment: heterotrophic aerobic populations. Appl Environ Microbiol 70:7378–7387 [CrossRef]
    [Google Scholar]
  14. Tiago I., Pires C., Mendes V., Morais P. V., da Costa M., Verissimo A. 2005; Microcella putealis gen. nov., sp. nov., a gram-positive alkaliphilic bacterium isolated from a nonsaline alkaline groundwater. Syst Appl Microbiol 28:479–487 [CrossRef]
    [Google Scholar]
  15. Tindall B. J. 1989; Fully saturated menaquinones in the archaebacterium Pyrobaculum islandicum . FEMS Microbiol Lett 60:251–254 [CrossRef]
    [Google Scholar]
  16. Yoon J. H., Kang S. S., Lee K. C., Kho Y. H., Choi S. H., Kang K. H., Park Y. H. 2001; Bacillus jeotgali sp. nov., isolated from jeotgal, Korean traditional fermented seafood. Int J Syst Evol Microbiol 51:1087–1092 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.64281-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64281-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error