1887

Abstract

Gram-negative, curved, motile bacteria (strains EqF1 and EqF2) were isolated from faecal samples from two clinically healthy horses. Both strains possessed a single, monopolar, sheathed flagellum and were urease-negative. The novel strains grew at 37 °C under microaerobic conditions and were positive for oxidase, catalase and alkaline phosphatase activities. The isolates reduced nitrate to nitrite, but -glutamyl transpeptidase activity was not detected. The novel isolates did not grow at 42 °C or on media containing 1 % glycine. They were resistant to cephalotin and nalidixic acid and susceptible to metronidazole. Analysis of the 16S and 23S rRNA gene sequences of the two novel strains identified them as representing a single species within the genus . In terms of 16S rRNA gene sequence similarity, and were the most closely related species (98 % similarity). 23S rRNA gene sequence analysis also classified strains EqF1 and EqF2 within the enterohepatic division of the genus , but only 94 % similarity was detected with and , which are helicobacters with unsheathed flagella. The most closely related species in terms of 23S rRNA gene sequence similarity was (95 %). Numerical analysis of whole-cell protein extracts by SDS-PAGE was performed and the novel isolates were clearly differentiated from , , and other species of the genus . This finding was also confirmed by sequence analysis of the gene. On the basis of these genetic, biochemical and protein data, the isolates are classified as representing a novel species, for which the name sp. nov. is proposed (type strain EqF1=LMG 23362=CCUG 52199).

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2007-02-01
2019-10-14
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Figures showing transmission electron microscope images of cells of strains EqF1 and EqF2 and phylogenetic trees based on 16S rRNA, 23S rRNA and gene sequences. [PDF](773 KB)

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Distance matrix analyses for the 16S rRNA, 23S rRNA and gene sequences. [PDF](29 KB)

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