1887

Abstract

A novel bacterium, designated strain H3-R18, was isolated from seashore sand collected from Homi cape, Pohang city, Korea. Cells were Gram-negative, aerobic, non-motile, cream-coloured, mesophilic and slightly halotolerant. 16S rRNA gene sequence analysis indicated that the organism was a member of the genus , but the sequence showed ⩽96.3 % sequence similarity to that of the type strains of all recognized species. Highest sequence similarities were to CFML 97-391 (96.3 %) and CIP 105470 (96.3 %). The major fatty acids were summed feature 3 and C, with lesser amounts of C, C 3-OH, Cω7 and C. The major isoprenoid quinone was Q-9. The DNA G+C content was 64.0 mol%. The phylogenetic, phenotypic and genetic properties of strain H3-R18 suggest that it represents a novel species, for which the name sp. nov. is proposed. The type strain is H3-R18 (=KACC 11517=DSM 17875).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.64274-0
2006-09-01
2024-11-10
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/56/9/2153.html?itemId=/content/journal/ijsem/10.1099/ijs.0.64274-0&mimeType=html&fmt=ahah

References

  1. Baïda N., Yazourh A., Singer E., Izard D. 2001; Pseudomonas brenneri sp. nov., a new species isolated from natural mineral waters. Res Microbiol 152:493–502 [CrossRef]
    [Google Scholar]
  2. Baumann P., Bouditch R. D., Baumann L., Beaman B. 1983; Taxonomy of marine Pseudomonas species: P. stanieri sp. nov.; P. perfectomarina sp. nov., nom. rev.; P. nautica ; and P. doudoroffii . Int J Syst Bacteriol 33:857–865 [CrossRef]
    [Google Scholar]
  3. Bennasar A., Rosselló-Mora R., Lalucat J., Moore E. R. B. 1996; 16S rDNA sequence analysis relative to genomovars of Pseudomonas stutzeri and proposal of Pseudomonas balearica sp. nov. Int J Syst Bacteriol 49:200–205
    [Google Scholar]
  4. Breznak J. A., Costilow R. N. 1994; Physicochemical factors in growth. In Methods for General and Molecular Bacteriology pp  137–154 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  5. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov. a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [CrossRef]
    [Google Scholar]
  6. King E. O., Ward M. K., Rainey D. E. 1954; Two simple media for demonstration of pyocyanin and fluorescein. J Lab Clin Med 44:301–307
    [Google Scholar]
  7. Kodama K., Odama K., Kimura N., Komagata K. 1985; Two new species of Pseudomonas : P. oryzihabitans isolated from rice paddy and clinical specimens and P. luteola isolated from clinical specimens. Int J Syst Bacteriol 35:467–474 [CrossRef]
    [Google Scholar]
  8. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [CrossRef]
    [Google Scholar]
  9. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  10. Migula W. 1894; über ein neues System der Bakterien. Arb Bakteriol Inst Karlsruhe 1:235–238
    [Google Scholar]
  11. Palleroni N. J. 1984; Genus I. Pseudomonas Migula 1984, 237AL . In Bergey's Manual of Systematic Bacteriology vol. 1 pp  141–199 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  12. Romanenko L. A., Uchino M., Falsen E., Frolova G. M., Zhukova N. V., Mikhailov V. V. 2005; Pseudomonas pachastrellae sp. nov., isolated from a marine sponge. Int J Syst Evol Microbiol 55:919–924 [CrossRef]
    [Google Scholar]
  13. Rosselló-Mora R. A., García-Valdés E., Lalucat J. 1993; Taxonomic relationship between Pseudomonas perfectomarina ZoBell and Pseudomonas stutzeri . Int J Syst Bacteriol 43:852–854 [CrossRef]
    [Google Scholar]
  14. Simpson J. M., Santo Domingo J. W., Donald J., Reasoner D. J. 2004; Assessment of equine fecal contamination: the search for alternative bacterial source-tracking targets. FEMS Microbiol Ecol 47:65–75 [CrossRef]
    [Google Scholar]
  15. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp  607–654 Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  16. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [CrossRef]
    [Google Scholar]
  17. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  18. Verhille S., Baída N., Dabboussi F., Hamze M., Izard D., Leclerc H. 1999; Pseudomonas gessardii sp. nov. and Pseudomonas migulae sp. nov. two new species isolated from natural mineral waters. Int J Syst Bacteriol 49:1559–1572 [CrossRef]
    [Google Scholar]
  19. Yumoto I., Yamazaki K., Hishinuma M., Nodasaka Y., Suemori A., Nakajima K., Inoue N., Kawasaki K. 2001; Pseudomonas alcaliphila sp. nov., a novel facultatively psychrophilic alkaliphile isolated from seawater. Int J Syst Evol Microbiol 51:349–355
    [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.64274-0
Loading
/content/journal/ijsem/10.1099/ijs.0.64274-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error