1887

Abstract

A novel bacterium, designated strain H3-R18, was isolated from seashore sand collected from Homi cape, Pohang city, Korea. Cells were Gram-negative, aerobic, non-motile, cream-coloured, mesophilic and slightly halotolerant. 16S rRNA gene sequence analysis indicated that the organism was a member of the genus , but the sequence showed ⩽96.3 % sequence similarity to that of the type strains of all recognized species. Highest sequence similarities were to CFML 97-391 (96.3 %) and CIP 105470 (96.3 %). The major fatty acids were summed feature 3 and C, with lesser amounts of C, C 3-OH, Cω7 and C. The major isoprenoid quinone was Q-9. The DNA G+C content was 64.0 mol%. The phylogenetic, phenotypic and genetic properties of strain H3-R18 suggest that it represents a novel species, for which the name sp. nov. is proposed. The type strain is H3-R18 (=KACC 11517=DSM 17875).

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2006-09-01
2019-12-08
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References

  1. Baïda, N., Yazourh, A., Singer, E. & Izard, D. ( 2001; ). Pseudomonas brenneri sp. nov., a new species isolated from natural mineral waters. Res Microbiol 152, 493–502.[CrossRef]
    [Google Scholar]
  2. Baumann, P., Bouditch, R. D., Baumann, L. & Beaman, B. ( 1983; ). Taxonomy of marine Pseudomonas species: P. stanieri sp. nov.; P. perfectomarina sp. nov., nom. rev.; P. nautica; and P. doudoroffii. Int J Syst Bacteriol 33, 857–865.[CrossRef]
    [Google Scholar]
  3. Bennasar, A., Rosselló-Mora, R., Lalucat, J. & Moore, E. R. B. ( 1996; ). 16S rDNA sequence analysis relative to genomovars of Pseudomonas stutzeri and proposal of Pseudomonas balearica sp. nov. Int J Syst Bacteriol 49, 200–205.
    [Google Scholar]
  4. Breznak, J. A. & Costilow, R. N. ( 1994; ). Physicochemical factors in growth. In Methods for General and Molecular Bacteriology, pp. 137–154. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  5. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  6. King, E. O., Ward, M. K. & Rainey, D. E. ( 1954; ). Two simple media for demonstration of pyocyanin and fluorescein. J Lab Clin Med 44, 301–307.
    [Google Scholar]
  7. Kodama, K., Odama, K., Kimura, N. & Komagata, K. ( 1985; ). Two new species of Pseudomonas: P. oryzihabitans isolated from rice paddy and clinical specimens and P. luteola isolated from clinical specimens. Int J Syst Bacteriol 35, 467–474.[CrossRef]
    [Google Scholar]
  8. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  9. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  10. Migula, W. ( 1894; ). Über ein neues System der Bakterien. Arb Bakteriol Inst Karlsruhe 1, 235–238.
    [Google Scholar]
  11. Palleroni, N. J. ( 1984; ). Genus I. Pseudomonas Migula 1984 , 237AL. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 141–199. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  12. Romanenko, L. A., Uchino, M., Falsen, E., Frolova, G. M., Zhukova, N. V. & Mikhailov, V. V. ( 2005; ). Pseudomonas pachastrellae sp. nov., isolated from a marine sponge. Int J Syst Evol Microbiol 55, 919–924.[CrossRef]
    [Google Scholar]
  13. Rosselló-Mora, R. A., García-Valdés, E. & Lalucat, J. ( 1993; ). Taxonomic relationship between Pseudomonas perfectomarina ZoBell and Pseudomonas stutzeri. Int J Syst Bacteriol 43, 852–854.[CrossRef]
    [Google Scholar]
  14. Simpson, J. M., Santo Domingo, J. W., Donald, J. & Reasoner, D. J. ( 2004; ). Assessment of equine fecal contamination: the search for alternative bacterial source-tracking targets. FEMS Microbiol Ecol 47, 65–75.[CrossRef]
    [Google Scholar]
  15. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  16. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  17. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  18. Verhille, S., Baída, N., Dabboussi, F., Hamze, M., Izard, D. & Leclerc, H. ( 1999; ). Pseudomonas gessardii sp. nov. and Pseudomonas migulae sp. nov., two new species isolated from natural mineral waters. Int J Syst Bacteriol 49, 1559–1572.[CrossRef]
    [Google Scholar]
  19. Yumoto, I., Yamazaki, K., Hishinuma, M., Nodasaka, Y., Suemori, A., Nakajima, K., Inoue, N. & Kawasaki, K. ( 2001; ). Pseudomonas alcaliphila sp. nov., a novel facultatively psychrophilic alkaliphile isolated from seawater. Int J Syst Evol Microbiol 51, 349–355.
    [Google Scholar]
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vol. , part 9, pp. 2153-2156

Transmission electron micrograph of a cell of strain H3-R18 . [PDF](475 KB)



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