Seven Gram-positive, coryneform bacteria with virtually identical whole-organism protein patterns were isolated from the surface of smear-ripened cheeses. Representatives of these strains were the subject of a polyphasic study designed to establish their taxonomic status. The organisms formed a distinct branch in the Microbacteriaceae 16S rRNA gene tree and were most closely related to members of the genus Agrococcus, sharing sequence similarities of 95.4–98.7 %. The chemotaxonomic profiles of the strains were consistent with their classification in the genus Agrococcus. The combined genotypic and phenotypic data show that the isolates should be classified in the genus Agrococcus as representatives of a novel species. The name Agrococcus casei sp. nov. is proposed for this taxon. Isolate R-17892t2T (=DSM 18061T=LMG 22410T) is the type strain of Agrococcus casei sp. nov.
BrennanN. M.,
BrownR.,
GoodfellowM.,
WardA. C.,
BeresfordT. P.,
VancanneytM.,
CoganT. M.,
FoxP. F.2001a; Microbacterium gubbeenense sp. nov., from the surface of a smear-ripened cheese. Int J Syst Evol Microbiol 51:1969–1976[CrossRef]
BrennanN. M.,
BrownR.,
GoodfellowM.,
WardA. C.,
BeresfordT. P.,
SimpsonP. J.,
FoxP. F.,
CoganT. M.2001b; Corynebacterium mooreparkense sp. nov. and Corynebacterium casei sp. nov., isolated from the surface of a smear-ripened cheese. Int J Syst Evol Microbiol 51:843–852[CrossRef]
BrennanN. M.,
WardA. C.,
BeresfordT. P.,
FoxP. F.,
GoodfellowM.,
CoganT. M.2002; Biodiversity of the bacterial flora on the surface of a smear cheese. Appl Environ Microbiol 68:820–830[CrossRef]
CarnioM. C.,
EppertI.,
SchererS.1999; Analysis of the bacterial surface ripening flora of German and French smeared cheeses with respect to their anti-listerial potential. Int J Food Microbiol 47:89–97[CrossRef]
CollinsM. D.,
KroppenstedtR. M.1987; Structures of the partially saturated menaquinones of Glycomyces rutgersensis
. FEMS Microbiol Lett 44:215–219[CrossRef]
FelsensteinJ.1993phylip (phylogenetic inference package), version 3.5c. Distributed by the author. Department of Genome Sciences University of Washington; Seattle, USA:
FitchW. M.,
MargoliashE.1967; Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155:279–284[CrossRef]
GeversD.,
HuysG.,
SwingsJ.2001; Applicability of rep -PCR fingerprinting for identification of Lactobacillus species. FEMS Microbiol Lett 205:31–36[CrossRef]
GordonR. E.1968; The taxonomy of soil bacteria. In The Ecology of Soil Bacteria pp 293–321 Edited by
GrayT. R. G.,
ParkinsonD.
Liverpool: Liverpool University Press;
GrothI.,
SchumannP.,
WeissN.,
MartinK.,
RaineyF. A.1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall.. Int J Syst Bacteriol 46:234–239[CrossRef]
HughR.,
LeifsonE.1953; The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram-negative bacteria. J Bacteriol 66:24–26
IrlingerF.,
BimetF.,
DelettreJ.,
LefevreM.,
GrimontP. A. D.2005; Arthrobacter bergerei sp. nov. and Arthrobacter arilaitensis sp. nov., novel coryneform species isolated from the surfaces of cheeses. Int J Syst Evol Microbiol 55:457–462[CrossRef]
JukesT. H.,
CantorC. R.1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol 3 pp 21–132 Edited by
MunroH. N.
New York: Academic Press;
KroppenstedtR. M.1985; Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics pp 173–179 Edited by
GoodfellowM.,
MinnikinD. E.
London: Academic Press;
LinY. C.,
UemoriK.,
De BrielD. A.,
ArunpairojanaV.,
YokotaA.2004; Zimmermannella helvola gen. nov., sp. nov., Zimmermannella alba sp. nov., Zimmermannella bifida sp. nov., Zimmermannella faecalis sp. nov. and Leucobacter albus sp. nov., novel members of the family Microbacteriaceae
. Int J Syst Evol Microbiol 54:1669–1676[CrossRef]
MayilrajS.,
SureshK.,
SchumannP.,
KroppenstedtR. M.,
SainiH. S.2006; Agrococcus lahaulensis sp. nov., isolated from a cold desert of the Indian Himalayas. Int J Syst Evol Microbiol 56:1807–1810[CrossRef]
MesbahM.,
PremachandranU.,
WhitmanW. B.1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167[CrossRef]
MinnikinD. E.,
O'DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalK.,
ParlettJ. H.1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241[CrossRef]
PitcherD. G.,
SaundersN. A.,
OwenR. J.1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156[CrossRef]
Piton-MalleretC.,
GorrieriM.1992; Nature et variabilité de la flore microbienne dans la morge des fromages de Compté et de Beaufort. Lait 72:143–164 (in French [CrossRef]
PotB.,
VandammeP.,
KerstersK.1994; Analysis of electrophoretic whole-organism protein fingerprints. In Modern Microbial Methods (Chemical Methods in Prokaryotic Systematics Series) pp 493–521 Edited by
GoodfellowM.,
O'DonnellA. G.
Chichester: Wiley;
SchaalK. P.1985; Identification of clinically significant actinomycetes and related bacteria using chemical techniques. In Chemical Methods in Bacterial Systematics pp 359–381 Edited by
GoodfellowM.,
MinnikinD. E.
London: Academic Press;
SierraG.1957; A simple method for the detection of lipolytic activity of micro-organisms and some observations on the influence of contact between cells and fatty substrates. Antonie van Leeuwenhoek 23:15–22[CrossRef]
UchidaK.,
KudoT.,
SuzukiK.,
NakaseT.1999; A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45:49–56[CrossRef]
Valdés-StauberN.,
SchererS.,
SeilerH.1997; Identification of yeasts and coryneform bacteria from the surface microflora of brick cheeses. Int J Food Microbiol 34:115–129[CrossRef]
WieserM.,
SchumannP.,
MartinK.,
AltenburgerP.,
BurghardtJ.,
LubitzW.,
BusseH.-J.1999; Agrococcus citreus sp. nov., isolated from a medieval wall painting of the chapel of Castle Herberstein (Austria). Int J Syst Bacteriol 49:1165–1170[CrossRef]
WilliamsS. T.,
GoodfellowM.,
AldersonG.,
WellingtonE. M. H.,
SneathP. H. A.,
SackinM. J.1983; Numerical classification of Streptomyces and related genera. J Gen Microbiol 129:1743–1813
ZlamalaC.,
SchumannP..,
KämpferP.,
Rosselló-MoraR.,
LubitzW.,
BusseH.-J.2002; Agrococcus baldri sp. nov., isolated from the air in the ‘Virgilkapelle’ in Vienna. Int J Syst Evol Microbiol 52:1211–1216[CrossRef]