1887

Abstract

A Gram-positive bacterium, designated B22, was isolated from potting soil produced in Portugal. This organism is a catalase-positive, oxidase-negative, motile, spore-forming, aerobic rod that grows optimally at 37 °C and pH 8.0–8.5. Optimal growth occurs in media containing 1 % (w/v) NaCl, although the organism can grow in 0–8 % NaCl. The cell wall peptidoglycan is of the A4 type with a cross-linkage containing -Asp. The major respiratory quinone is menaquinone 7 and the major fatty acids are anteiso-15 : 0, anteiso-17 : 0 and iso-15 : 0. The DNA G+C content is 37.9 mol%. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain B22 formed a new branch within the family . The novel isolate is phylogenetically closely related to members of genera of moderately halophilic bacilli and formed a coherent cluster with species of the genera , , and , supported by bootstrap analysis at a confidence level of 71 %. Strain B22 exhibited 16S rRNA gene pairwise sequence similarity values of 94.7–94.3 % with members of the genus , 95.1–92.8 % with members of the genus , 94.7–93.2 % with members of the genus and 93.1–92.3 % with members of the genus . On the basis of phylogenetic analysis and physiological and biochemical characteristics, it is proposed that strain B22 represents a novel species in a new genus, gen. nov., sp. nov. Strain B22 (=LMG 23148=CIP 108857) is the type strain of .

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2006-08-01
2020-09-28
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