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Abstract

Two Gram-positive, non-motile, non-spore-forming and rod-shaped actinomycete strains, KSL-113 and KSL-133, were isolated from an alkaline soil in Korea, and their taxonomic positions were investigated by using a polyphasic approach. The strains grew optimally at 30 °C and pH 9.0. Phenotypic, phylogenetic and genetic similarities indicated that strains KSL-113 and KSL-133 represent the same species. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains KSL-113 and KSL-133 fell within the family of the suborder , the highest 16S rRNA gene sequence similarity values (98.2 %) being obtained with respect to CV-2. The 16S rRNA gene sequence similarities between strains KSL-113 and KSL-133 and the other members of the family used in the phylogenetic analysis were less than 96.0 %. Strains KSL-113 and KSL-133 could be clearly distinguished from members of the family on the basis of differences in chemotaxonomic properties, including the predominant menaquinone type, the cell-wall peptidoglycan type and the fatty acid profile. Accordingly, on the basis of the combined phenotypic, chemotaxonomic and phylogenetic data, strains KSL-113 and KSL-133 constitute a novel genus and species of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is KSL-113 (=KCTC 19126=CIP 108920).

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2006-10-01
2020-01-25
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Percentage cellular fatty acid compositions of strains KSL-113 and KSL-133. [PDF](20 KB)



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