The taxonomic positions of two actinobacterial strains isolated from Mariana Trench sediment were established using a combination of genotypic and phenotypic data. The strains, isolates MT2.1T and MT2.2T, formed a distinct phyletic line in the Micrococcineae 16S rRNA gene tree together with Dermacoccus abyssi NCIMB 14084T. The isolates had chemical and phenotypic properties typical of members of the genus Dermacoccus and could be distinguished sharply from one another and from the type strains of Dermacoccus abyssi and Dermacoccus nishinomiyaensis using DNA–DNA relatedness data. A range of phenotypic properties served to distinguish the two novel strains from one another and from the type strains of established Dermacoccus species. The G+C contents of the DNAs of strains MT2.1T and MT2.2T were 66.8 and 69.1 mol%, respectively. It is evident that the two isolates merit recognition as novel species within the genus Dermacoccus. The names proposed for these taxa are Dermacoccus barathri sp. nov. (type strain MT2.1T=DSM 17574T=NCIMB 14081T) and Dermacoccus profundi sp. nov. (type strain MT2.2T=DSM 17575T=NCIMB 14084T).
BeckerK.,
SchumannP.,
WüllenweberJ.,
SchulteM.,
WeilH.-P.,
StackebrandtE.,
PetersG.,
von EiffC.2002; Kytococcus schroeteri sp. nov., a novel Gram-positive actinobacterium isolated from a human clinical source. Int J Syst Evol Microbiol 52:1609–1614[CrossRef]
CollinsM. D.1994; Isoprenoid quinones. In Chemical Methods in Prokaryotic Systematics pp 265–309 Edited by
GoodfellowM.,
O'DonnellA. G.
Chichester: Wiley;
CorderoM. R.,
ZumalacárreguiJ. M.2000; Characterization of Micrococcaceae isolated from salt used for Spanish dry-cured ham. Lett Appl Microbiol 31:303–306[CrossRef]
De la RosaM. C.,
MohinoM. R.,
MohinoM.,
MossoM. A.1990; Characteristics of micrococci and staphylococci isolated from semi-preserved meat products. Food Microbiol 7:207–215[CrossRef]
EzakiT.,
HashimotoY.,
YabuuchiE.1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229[CrossRef]
GrothI.,
SchumannP.,
RaineyF. A.,
MartinK.,
SchuetzeB.,
AugstenK.1997; Demetria terragena gen. nov., sp. nov. a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47:1129–1133[CrossRef]
HamidM. E.,
MinnikinD. E.,
GoodfellowM.,
RidellM.1993; Thin-layer chromatographic analysis of glycolipids and mycolic acids from Mycobacterium farcinogenes , Mycobacterium senegalense and related taxa. Zentralbl Bakteriol 279:354–367[CrossRef]
JukesT. H.,
CantorC. R.1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol 3 pp 21–132 Edited by
MunroH. N.
New York: Academic Press;
KloosW. E.,
TornabeneT. G.,
SchleiferK. H.1974; Isolation and characterization of micrococci from human skin, including two new species: Micrococcus lylae and Micrococcus kristinae
. Int J Syst Bacteriol 24:79–101[CrossRef]
McNabbA.,
ShuttleworthR.,
BehmeR.,
ColbyW. D.1997; Fatty acid characterization of rapidly growing pathogenic aerobic actinomycetes as a means of identification. J Clin Microbiol 35:1361–1368
MinnikinD. E.,
O'DonnellA. G.,
GoodfellowM.,
AldersonG.,
AthalyeM.,
SchaalA.,
ParlettJ. H.1984; An integrated procedure for the extraction of isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241[CrossRef]
OdaM.1935; Bacteriological studies on waters used for brewing saké (part 6). I. Bacteriological studies on “miyamizu” (8) and (9). Micrococcus and Actinomyces isolated from “miyamizu”. Jozogaku Zasshi 13, 1202–1228 (in Japanese)
PapamanoliE.,
KotzekidouP.,
TzanetakisN.,
Litopoulou-TzanetakiE.2002; Characterization of Micrococcaceae isolated from dry fermented sausage. Food Microbiol 19:441–449[CrossRef]
Pathom-areeW.,
StachJ. E. M.,
WardA. C.,
HorikoshiK.,
BullA. T.,
GoodfellowM.2006b; Diversity of actinomycetes isolated from Challenger Deep sediment (10,898 m) from the Mariana Trench. Extremophiles 10:181–189[CrossRef]
SchumannP.,
SpröerC.,
BurghardtJ.,
KovacsG.,
StackebrandtE.1999; Reclassification of the species Kocuria erythromyxa (Brooks and Murray 1981) as Kocuria rosea (Flügge 1886). Int J Syst Bacteriol 49:393–396[CrossRef]
StackebrandtE.,
SchumannP.2000; Description of Bogoriellaceae fam.nov., Dermacoccaceae fam. nov., Rarobacteraceae fam. nov. and Sanguibacteraceae fam. nov.and emendation of some families of the suborder Micrococcineae
. Int J Syst Evol Microbiol 50:1279–1285[CrossRef]
StackebrandtE.,
KochC.,
GvozdiakO.,
SchumannP.1995; Taxonomic dissection of the genus Micrococcus : Kocuria gen.nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend. Int J Syst Bacteriol 45:682–692[CrossRef]
SutcliffeI. C.2000; Characterisation of a lipomannan lipoglycan from the mycolic acid containing actinomycete Dietzia maris
. Antonie van Leeuwenhoek 78:195–201[CrossRef]
TamaokaJ.,
KomagataK.1984; Determination of DNA base composition by reverse-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128[CrossRef]
UchidaK.,
KudoT.,
SuzukiK. I.,
NakaseT.1999; A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45:49–56[CrossRef]
VickersJ. C.,
WilliamsS. T.,
RossG. W.1984; A taxonomic approach to selective isolation of streptomycetes from soil. In Biological, Biochemical and Biomedical Aspects of Actinomycetes pp 553–561 Edited by
Ortiz-OrtizL.,
BojalilL. F.,
YakoleffV.
Orlando: Academic Press;
WayneL. G.,
BrennerD. J.,
ColwellR. R.9 other authors1987; International committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464[CrossRef]