1887

Abstract

Two novel strains, GR20-10 and GR20-13, were isolated from soil using R2A medium. The soil sample was collected from a field in the Yeongju region of Korea that was cultivated with Korean ginseng. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains formed a cluster with several uncultured bacterial clones and with , , , and (recently reclassified as ) in the phylum . The level of 16S rRNA gene sequence similarity between the two novel strains was 98.9 %. Isolates GR20-10 and GR20-13 showed the highest sequence similarities to IFO 16041 (91.8 and 91.9 %, respectively) and ATCC 13524 (90.4 and 90.6 %, respectively). The whole-cell fatty acid profiles of the two isolates were similar and their major fatty acids were 15 : 0 iso, 17 : 0 iso 3-OH and 15 : 1 iso G. The major isoprenoid quinone of both strains was MK-7. The G+C contents of GR20-10 and GR20-13 were 45.8 and 44.3 mol%, respectively. DNA–DNA hybridization (57 % DNA–DNA hybridization value) and phenotypic data indicated that strains GR20-10 and GR20-13 each belong to a separate species. On the basis of phenotypic and phylogenetic data and genomic distinctiveness, strains GR20-10 and GR20-13 represent two novel species in a novel genus in the phylum ; the names gen. nov., sp. nov. (the type species; type strain GR20-10=KACC 11465=DSM 17620) and sp. nov. (type strain GR20-13=KACC 11466=DSM 17621) are proposed.

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2006-08-01
2021-03-03
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