1887

Abstract

Three strains, MAFF 210191, G24103 and G24116, assumed to be members of two novel species, were isolated from several rhizosphere habitats in different parts of Japan. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolates formed a distinct monophyletic group together with the two known species of the genus , suggesting that the isolates have a close affiliation with this genus. In this study, a polyphasic approach was used to characterize and compare the three isolates with the two species of the genus , and . All three isolates were aerobic, Gram-negative, motile and non-sporulating and they ranged in shape from spherical to short rods. The cells multiplied by budding and utilized a wide variety of monosaccharides, disaccharides and sugar alcohols as sole carbon and energy sources, but they did not utilize C compounds, salicin or -melezitose. The strains were inhibited by --alanine and glycine (both at 10 mM). The major cellular fatty acids were C cyclo 8, C, C and C 7. The three isolates shared <12 % and <11 % DNA–DNA relatedness with DSM 5896 and DSM 16812, respectively. The G+C content of the isolates (61–62 mol%) was also significantly lower than those of the two previously characterized species. In spite of many morphological, physiological and chemotaxonomic similarities among the three isolates, strain MAFF 210191 could be differentiated from strains G24103 and G24116 on the basis of 16S rRNA gene sequence divergence, DNA–DNA relatedness (<46 %) and gelatin hydrolysis. Two novel species are therefore proposed, namely sp. nov., with the type strain MAFF 210191 (=DSM 18385), and sp. nov., with the type strain G24103 (=NBRC 101365=NCIMB 14143) and also including strain G24116 (=NBRC 101366=NCIMB 14144). Emended descriptions of the genus and are also presented.

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2007-03-01
2019-10-14
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vol. , part 3, pp. 552 - 557

Assimilation of substrates by the three isolates and representatives of the genus .

DNA–DNA hybridization between the three isolates and representatives of the genus .

Cellular fatty acid profiles of the three isolates and representatives of the genus .

[PDF file of Supplementary Tables S1-S3](30 KB)



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