1887

Abstract

Three novel bacterial strains were isolated from a soil sample collected in Japan by culture on a GPM agar plate supplemented with superoxide dismutase and catalase. The strains were Gram-positive, catalase-positive, non-motile bacteria with -ornithine as a diagnostic diamino acid of the peptidoglycan. The acyl type of the peptidoglycan was -glycolyl. The major menaquinones were MK-12, 13 and 14. Mycolic acids were not detected. G+C contents of the DNA were in the range 69–71 mol%. Comparative 16S rRNA gene sequence analysis revealed that the isolates belonged to the genus and were closely related to , , , and . However, , and clearly differed from the isolated strains based on the presence of -lysine as the cell-wall diamino acid and various other chemotaxonomic characteristics. Levels of DNA–DNA relatedness showed that the isolated strains represented three separate genomic species. Based on both phenotypic and genotypic data, the following novel species of the genus are proposed: sp. nov. (type strain KV-483=NRRL B-24453=NBRC 101278), sp. nov. (type strain KV-488=NRRL B-24452=NBRC 101279) and sp. nov. (type strain KV-492=NRRL B-24451=NBRC 101280).

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2006-09-01
2024-10-03
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References

  1. Becker B., Lechevalier M. P., Lechevalier H. A. 1965; Chemical composition of cell-wall preparation from strains of various form-genera of aerobic actinomycetes. Appl Microbiol 13:236–243
    [Google Scholar]
  2. Behrendt U., Ulrich A., Schumann P. 2001; Description of Microbacterium foliorum sp. nov. and Microbacterium phyllosphaerae sp. nov., isolated from the phyllosphere of grasses and the surface litter after mulching the sward, and reclassification of Aureobacterium resistens . (Funke et al . 1998) as Microbacterium resistens comb. nov. Int J Syst Evol Microbiol 51, 1267–1276
  3. Collins M. D., Bradbury J. F. 1992; The genera Agromyces , Aureobacterium , Clavibacter , Curtobacterium , and Microbacterium . In The Prokaryotes , 2nd edn. pp  1355–1368 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. Berlin: Springer;
    [Google Scholar]
  4. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. 1977; Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230 [CrossRef]
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  6. Kawamoto I., Oka T., Nara T. 1981; Cell wall composition of Micromonospora olivoasterospora , Micromonospora sagamiensis , and related organisms. J Bacteriol 146:527–534
    [Google Scholar]
  7. Kimura M., Ohta T. 1972; On the stochastic model for estimation of mutation distance between homologous proteins. J Mol Evol 2:87–90 [CrossRef]
    [Google Scholar]
  8. Lee J. S., Lee K. C., Park Y. H. 2006; Microbacterium koreense sp. nov., from sea water in the South Sea of Korea. Int J Syst Evol Microbiol 56:423–427 [CrossRef]
    [Google Scholar]
  9. Orla-Jensen S. 1919 The Lactic Acid Bacteria Copenhagen: Host & Sons;
    [Google Scholar]
  10. Pridham T. G., Gottlieb D. 1948; The utilization of carbon compounds by some Actinomycetales as an aid for species determination. J Bacteriol 56:107–114
    [Google Scholar]
  11. Saito H., Miura K. 1983; Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72:619–629
    [Google Scholar]
  12. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  13. Suzuki K., Komagata K. 1983; Taxonomic significance of cellular fatty acid composition in some coryneform bacteria. Int J Syst Bacteriol 33:188–200 [CrossRef]
    [Google Scholar]
  14. Takahashi Y., Katoh S., Shikura N., Tomoda H., Omura S. 2003; Superoxide dismutase produced by soil bacteria increases bacterial colony growth from soil samples. J Gen Appl Microbiol 49:263–266 [CrossRef]
    [Google Scholar]
  15. Takeuchi M., Hatano K. 1998; Union of the genera Microbacterium Orla-Jensen and Aureobacterium Collins et al . in a redefined genus Microbacterium . Int J Syst Bacteriol 48:739–747 [CrossRef]
    [Google Scholar]
  16. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [CrossRef]
    [Google Scholar]
  17. Tamaoka J., Katayama-Fujimura Y., Kuraishi H. 1983; Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54:31–36 [CrossRef]
    [Google Scholar]
  18. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  19. Tomiyasu I. 1982; Mycolic acid composition and thermally adaptive changes in Nocardia asteroids . J Bacteriol 151:828–837
    [Google Scholar]
  20. Uchida K., Aida K. 1977; Acyl type of bacterial cell wall: its simple identification by a colorimetric method. J Gen Appl Microbiol 23:249–260 [CrossRef]
    [Google Scholar]
  21. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [CrossRef]
    [Google Scholar]
  22. Yokota A., Takeuchi M., Weiss N. 1993a; Proposal of two new species in the genus Microbacterium : Microbacterium dextranolyticum sp. nov. and Microbacterium aurum sp. nov. Int J Syst Bacteriol 43:549–554 [CrossRef]
    [Google Scholar]
  23. Yokota A., Takeuchi M., Sakane T., Weiss N. 1993b; Proposal of six new species in the genus Aureobacterium and transfer of Flavobacterium esteraromaticum Omelicanski to the genus Aureobacterium as Aureobacterium esteraromaticum comb. nov. Int J Syst Bacteriol 43:555–564 [CrossRef]
    [Google Scholar]
  24. Yu L., Takahashi Y., Matsumoto A., Seino A., Iwai Y., Omura S. 2002; Application of PCR for selection of gram-positive bacteria with high DNA G+C content among new isolates. Actinomycetologica 16:1–5 [CrossRef]
    [Google Scholar]
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