Lawson . 2001 is a later heterotypic synonym of Tanasupawat . 2000 Free

Abstract

The applicability of a multilocus sequence analysis (MLSA)-based identification system for lactobacilli was evaluated. Two housekeeping genes that code for the phenylalanyl-tRNA synthase -subunit () and RNA polymerase -subunit () were sequenced and analysed for members of the species group. The type strains of and were investigated further using a third gene that encodes the -subunit of ATP synthase (). The MLSA data revealed close relatedness between and , with 99.8–100 % , and gene sequence similarities. Comparison of the 16S rRNA gene sequences of the type strains of the two species confirmed the close relatedness (99.8 % gene sequence similarity) between the two taxa. Similar phenotypes and high DNA–DNA binding values in the range of 84 to 97.5 % confirmed that and are synonymous species. On the basis of the present study, it is proposed that is a later heterotypic synonym of .

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2006-07-01
2024-03-29
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References

  1. Chavagnat F., Haueter M., Jimeno J., Casey M. G. 2002; Comparison of partial tuf gene sequences for the identification of lactobacilli. FEMS Microbiol Lett 217:177–183 [CrossRef]
    [Google Scholar]
  2. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [CrossRef]
    [Google Scholar]
  3. Goris J., Suzuki K., De Vos P., Nakase T., Kersters K. 1998; Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44:1148–1153 [CrossRef]
    [Google Scholar]
  4. Lapage S. P., Sneath P. H. A., Lessel E. F., Skerman V. B. D., Seeliger H. P. R., Clark W. A. (editors) 1992 International Code of Nomenclature of Bacteria (1990 Revision). Bacteriological Code Washington, DC: American Society for Microbiology;
    [Google Scholar]
  5. Lawson P. A., Papademas P., Wacher C., Falsen E., Robinson R., Collins M. D. 2001; Lactobacillus cypricasei sp. nov., isolated from Halloumi cheese. Int J Syst Evol Microbiol 51:45–49
    [Google Scholar]
  6. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208–218 [CrossRef]
    [Google Scholar]
  7. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167 [CrossRef]
    [Google Scholar]
  8. Naser S., Thompson F. L., Hoste B., Gevers D., Vandemeulebroecke K., Cleenwerck I., Thompson C. C., Vancanneyt M., Swings J. 2005a; Phylogeny and identification of enterococci using atpA gene sequence analysis. J Clin Microbiol 43:2224–2230 [CrossRef]
    [Google Scholar]
  9. Naser S. M., Thompson F. L., Hoste B., Gevers D., Dawyndt P., Vancanneyt M., Swings J. 2005b; Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiology 151:2141–2150 [CrossRef]
    [Google Scholar]
  10. Naser S. M., Hagen K. E., Vancanneyt M., Cleenwerck I., Swings J., Tompkins T. A. 2006a; Lactobacillus suntoryeus Cachat and Priest 2005 is a later synonym of Lactobacillus helveticus (Orla-Jensen 1919) Bergey et al . 1925 (Approved Lists 1980). Int J Syst Evol Microbiol 56:355–360 [CrossRef]
    [Google Scholar]
  11. Naser S. M., Vancanneyt M., Hoste B., Snauwaert C., Vandemeulebroecke K., Swings J. 2006b; Reclassification of Enterococcus flavescens Pompei et al . 1992 as a later synonym of Enterococcus casseliflavus ( ex Vaughan et al . 1979) Collins et al . 1984, and Enterococcus saccharominimus Vancanneyt et al . 2004 as a later synonym of Enterococcus italicus Fortina et al . 2004. Int J Syst Evol Microbiol 56:413–416 [CrossRef]
    [Google Scholar]
  12. Pitcher D. G., Saunders N. A., Owen R. G. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156 [CrossRef]
    [Google Scholar]
  13. Stackebrandt E., Frederiksen W., Garrity G. M. 10 other authors 2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047 [CrossRef]
    [Google Scholar]
  14. Tanasupawat S., Shida O., Okada S., Komagata K. 2000; Lactobacillus acidipiscis sp. nov. and Weissella thailandensis sp. nov., isolated from fermented fish in Thailand. Int J Syst Evol Microbiol 50, 1479–1485 [CrossRef]
  15. Torriani S., Felis G. E., Dellaglio F. 2001; Differentiation of Lactobacillus plantarum , L. pentosus , and L. paraplantarum by recA gene sequence analysis and multiplex PCR assay with recA gene-derived primers. Appl Environ Microbiol 67:3450–3454 [CrossRef]
    [Google Scholar]
  16. Ventura M., Canchaya C., Meylan V., Klaenhammer T. R., Zink R. 2003; Analysis, characterization, and loci of the tuf genes in Lactobacillus and Bifidobacterium species and their direct application for species identification. Appl Environ Microbiol 69:6908–6922 [CrossRef]
    [Google Scholar]
  17. Zeigler D. R. 2003; Gene sequences useful for predicting relatedness of whole genomes in bacteria. Int J Syst Evol Microbiol 53:1893–1900 [CrossRef]
    [Google Scholar]
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