The applicability of a multilocus sequence analysis (MLSA)-based identification system for lactobacilli was evaluated. Two housekeeping genes that code for the phenylalanyl-tRNA synthase α-subunit (pheS) and RNA polymerase α-subunit (rpoA) were sequenced and analysed for members of the Lactobacillus salivarius species group. The type strains of Lactobacillus acidipiscis and Lactobacillus cypricasei were investigated further using a third gene that encodes the α-subunit of ATP synthase (atpA). The MLSA data revealed close relatedness between L. acidipiscis and L. cypricasei, with 99.8–100 % pheS, rpoA and atpA gene sequence similarities. Comparison of the 16S rRNA gene sequences of the type strains of the two species confirmed the close relatedness (99.8 % gene sequence similarity) between the two taxa. Similar phenotypes and high DNA–DNA binding values in the range of 84 to 97.5 % confirmed that L. acidipiscis and L. cypricasei are synonymous species. On the basis of the present study, it is proposed that Lactobacillus cypricasei is a later heterotypic synonym of Lactobacillus acidipiscis.
ChavagnatF.,
HaueterM.,
JimenoJ.,
CaseyM. G.2002; Comparison of partial tuf gene sequences for the identification of lactobacilli. FEMS Microbiol Lett 217:177–183[CrossRef]
EzakiT.,
HashimotoY.,
YabuuchiE.1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229[CrossRef]
GorisJ.,
SuzukiK.,
De VosP.,
NakaseT.,
KerstersK.1998; Evaluation of a microplate DNA-DNA hybridization method compared with the initial renaturation method. Can J Microbiol 44:1148–1153[CrossRef]
LapageS. P.,
SneathP. H. A.,
LesselE. F.,
SkermanV. B. D.,
SeeligerH. P. R.,
ClarkW. A.
(editors) 1992International Code of Nomenclature of Bacteria (1990 Revision). Bacteriological Code Washington, DC: American Society for Microbiology;
MesbahM.,
PremachandranU.,
WhitmanW. B.1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167[CrossRef]
NaserS. M.,
ThompsonF. L.,
HosteB.,
GeversD.,
DawyndtP.,
VancanneytM.,
SwingsJ.2005b; Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes. Microbiology 151:2141–2150[CrossRef]
NaserS. M.,
HagenK. E.,
VancanneytM.,
CleenwerckI.,
SwingsJ.,
TompkinsT. A.2006a; Lactobacillus suntoryeus Cachat and Priest 2005 is a later synonym of Lactobacillus helveticus (Orla-Jensen 1919) Bergey et al . 1925 (Approved Lists 1980). Int J Syst Evol Microbiol 56:355–360[CrossRef]
NaserS. M.,
VancanneytM.,
HosteB.,
SnauwaertC.,
VandemeulebroeckeK.,
SwingsJ.2006b; Reclassification of Enterococcus flavescens Pompei et al . 1992 as a later synonym of Enterococcus casseliflavus ( ex Vaughan et al . 1979) Collins et al . 1984, and Enterococcus saccharominimus Vancanneyt et al . 2004 as a later synonym of Enterococcus italicus Fortina et al . 2004. Int J Syst Evol Microbiol 56:413–416[CrossRef]
PitcherD. G.,
SaundersN. A.,
OwenR. G.1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156[CrossRef]
StackebrandtE.,
FrederiksenW.,
GarrityG. M.10 other authors2002; Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int J Syst Evol Microbiol 52:1043–1047[CrossRef]
TanasupawatS.,
ShidaO.,
OkadaS.,
KomagataK.2000; Lactobacillus acidipiscis sp. nov. and Weissella thailandensis sp. nov., isolated from fermented fish in Thailand. Int J Syst Evol Microbiol 50, 1479–1485 [CrossRef]
TorrianiS.,
FelisG. E.,
DellaglioF.2001; Differentiation of Lactobacillus plantarum , L. pentosus , and L. paraplantarum by recA gene sequence analysis and multiplex PCR assay with recA gene-derived primers. Appl Environ Microbiol 67:3450–3454[CrossRef]
VenturaM.,
CanchayaC.,
MeylanV.,
KlaenhammerT. R.,
ZinkR.2003; Analysis, characterization, and loci of the tuf genes in Lactobacillus and Bifidobacterium species and their direct application for species identification. Appl Environ Microbiol 69:6908–6922[CrossRef]