1887

Abstract

The applicability of a multilocus sequence analysis (MLSA)-based identification system for lactobacilli was evaluated. Two housekeeping genes that code for the phenylalanyl-tRNA synthase -subunit () and RNA polymerase -subunit () were sequenced and analysed for members of the species group. The type strains of and were investigated further using a third gene that encodes the -subunit of ATP synthase (). The MLSA data revealed close relatedness between and , with 99.8–100 % , and gene sequence similarities. Comparison of the 16S rRNA gene sequences of the type strains of the two species confirmed the close relatedness (99.8 % gene sequence similarity) between the two taxa. Similar phenotypes and high DNA–DNA binding values in the range of 84 to 97.5 % confirmed that and are synonymous species. On the basis of the present study, it is proposed that is a later heterotypic synonym of .

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2006-07-01
2019-10-23
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