1887

Abstract

A Gram-positive, non-motile, moderately halophilic, alkalitolerant actinobacterium, designated strain YIM 70179, was isolated from a saline soil sample collected from Xinjiang Province, north-west China, and was subjected to a polyphasic taxonomic study. The cell-wall peptidoglycan type of strain YIM 70179 was A4, -Lys–Gly–-Glu. Cells of the isolate contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown glycolipid, MK-8 as major menaquinone and anteiso-C, anteiso-C and iso-C as major fatty acids. The DNA G+C content was 68.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 70179 fell within the radiation of species of the genus . Levels of 16S rRNA gene sequence similarity between strain YIM 70179 and the type strains of recognized species were below 97 %, except to DSM 20541 (99.6 %), but these two strains exhibited a low level of DNA–DNA relatedness (18.4 %). Based on genetic and phenotypic evidence, it is proposed that strain YIM 70179 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 70179 (=DSM 16378 =KCTC 19048).

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2008-06-01
2020-01-21
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References

  1. Cerny, G. ( 1978; ). Studies on aminopeptidase for the distinction of Gram-negative from Gram-positive bacteria. Appl Microbiol Biotechnol 5, 113–122.[CrossRef]
    [Google Scholar]
  2. Collins, M. D., Lawson, P. A., Labrenz, M., Tindall, B. J., Weiss, N. & Hirsch, P. ( 2002; ). Nesterenkonia lacusekhoensis sp. nov., isolated from hypersaline Ekho Lake, East Antarctica, and emended description of the genus Nesterenkonia. Int J Syst Evol Microbiol 52, 1145–1150.[CrossRef]
    [Google Scholar]
  3. Cui, X. L., Mao, P. H., Zeng, M., Li, W. J., Zhang, L. P., Xu, L. H. & Jiang, C. L. ( 2001; ). Streptomonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51, 357–363.
    [Google Scholar]
  4. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  5. Delgado, O., Quillaguaman, J., Bakhtiar, S., Mattiasson, B., Gessesse, A. & Hatti-Kaul, R. ( 2006; ). Nesterenkonia aethiopica sp. nov., an alkaliphilic, moderate halophile isolated from an Ethiopian soda lake. Int J Syst Evol Microbiol 56, 1229–1232.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  7. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  8. Frank, H., Rettenmeier, A., Weicker, H., Nicholson, G. J. & Bayer, E. ( 1980; ). A new gas chromatographic method for determination of amino acid levels in human serum. Clin Chim Acta 105, 201–211.[CrossRef]
    [Google Scholar]
  9. Gram, H. C. ( 1884; ). Über die isolierte Färbung der Schizomyceten in Schnitt- und Trockenpräparaten. Fortschr Med 2, 185–189 (in German).
    [Google Scholar]
  10. Groth, I., Schumann, P., Rainey, F. A., Martin, K., Schuetze, B. & Augsten, K. ( 1997; ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 47, 1129–1133.[CrossRef]
    [Google Scholar]
  11. Groth, I., Schumann, P., Martin, K., Schuetze, B., Augsten, K., Kramer, I. & Stackebrandt, E. ( 1999; ). Ornithinicoccus hortensis gen. nov., sp. nov., a soil actinomycete which contains l-ornithine. Int J Syst Bacteriol 49, 1717–1724.[CrossRef]
    [Google Scholar]
  12. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  13. Jahnke, K. D. ( 1992; ). BASIC computer program for evaluation of spectroscopic DNA renaturation data from GILFORD SYSTEM 2600 spectrophotometer on a PC/XT/AT type personal computer. J Microbiol Methods 15, 61–73.[CrossRef]
    [Google Scholar]
  14. Kelly, K. L. ( 1964; ). Inter-Society Color Council – National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office.
  15. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  16. Kimura, M. ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  17. Kumar, S., Tamura, K., Jakobsen, I.-B. & Nei, M. ( 2001; ). mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef]
    [Google Scholar]
  18. Li, W. J., Chen, H. H., Zhang, Y. Q., Schumann, P., Stackebrandt, E., Xu, L. H. & Jiang, C. L. ( 2004; ). Nesterenkonia halotolerans sp. nov. and Nesterenkonia xinjiangensis sp. nov., actinobacteria from saline soils in the west of China. Int J Syst Evol Microbiol 54, 837–841.[CrossRef]
    [Google Scholar]
  19. Li, W. J., Chen, H. H., Zhang, Y. Q., Schumann, P., Stackebrandt, E., Xu, L. H. & Jiang, C. L. ( 2005; ). Nesterenkonia sandarakina sp. nov. and Nesterenkonia lutea sp. nov., novel actinobacteria, and emended description of the genus Nesterenkonia. Int J Syst Evol Microbiol 55, 463–466.[CrossRef]
    [Google Scholar]
  20. MacKenzie, S. L. ( 1987; ). Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70, 151–160.
    [Google Scholar]
  21. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  22. Miller, L. T. ( 1982; ). Single derivatization method for routine analysis of bacterial whole-cell, fatty acids methyl esters, including hydroxy acids. J Clin Microbiol 16, 584–586.
    [Google Scholar]
  23. Mota, R. R., Marquez, C., Arahal, D., Mellado, E. & Ventosa, A. ( 1997; ). Polyphasic taxonomy of Nesterenkonia halobia. Int J Syst Bacteriol 47, 1231–1235.[CrossRef]
    [Google Scholar]
  24. Onish, H. & Kamekura, M. ( 1972; ). Micrococcus halobius sp. nov. Int J Syst Bacteriol 22, 233–236.[CrossRef]
    [Google Scholar]
  25. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  26. Schleifer, K. H. ( 1985; ). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123–156.
    [Google Scholar]
  27. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  28. Sehgal, S. N. & Gibbons, N. E. ( 1960; ). Effect of metal ions on the growth of Halobacterium cutirubrum. Can J Microbiol 6, 165–169.[CrossRef]
    [Google Scholar]
  29. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  30. Smibert, R. M. & Krieg, N. R. ( 1981; ). General characterization. In Manual of Methods for General Bacteriology, pp. 409–443. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Washington, DC: American Society for Microbiology.
  31. Stackebrandt, E., Koch, C., Gvozdiak, O. & Schumann, P. ( 1995; ). Taxonomic dissection of the genus Micrococcus: Kocuria gen. nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend. Int J Syst Bacteriol 45, 682–692.[CrossRef]
    [Google Scholar]
  32. Staley, J. T. ( 1968; ). Prosthecomicrobium and Ancalomicrobium, new prosthecate freshwater bacteria. J Bacteriol 95, 1921–1944.
    [Google Scholar]
  33. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  34. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  35. Xu, P., Li, W. J., Xu, L. H. & Jiang, C. L. ( 2003; ). A microwave-based method for genomic DNA extraction from actinomycetes. Microbiology (Beijing) 30, 82–84 (in Chinese).
    [Google Scholar]
  36. Yoon, J. H., Jung, S. Y., Kim, W., Nam, S. W. & Oh, T. K. ( 2006; ). Nesterenkonia jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 56, 2587–2592.[CrossRef]
    [Google Scholar]
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